Experimental Immunization Based on Plasmodium Antigens Isolated by Antibody Affinity
Vaccines blocking malaria parasites in the blood-stage diminish mortality and morbidity caused by the disease. Here, we isolated antigens from total parasite proteins by antibody affinity chromatography to test an immunization against lethal malaria infection in a murine model. We used the sera of m...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
|
| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2015/723946 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832549929330933760 |
|---|---|
| author | Ali N. Kamali Patricia Marín-García Isabel G. Azcárate Antonio Puyet Amalia Diez José M. Bautista |
| author_facet | Ali N. Kamali Patricia Marín-García Isabel G. Azcárate Antonio Puyet Amalia Diez José M. Bautista |
| author_sort | Ali N. Kamali |
| collection | DOAJ |
| description | Vaccines blocking malaria parasites in the blood-stage diminish mortality and morbidity caused by the disease. Here, we isolated antigens from total parasite proteins by antibody affinity chromatography to test an immunization against lethal malaria infection in a murine model. We used the sera of malaria self-resistant ICR mice to lethal Plasmodium yoelii yoelii 17XL for purification of their IgGs which were subsequently employed to isolate blood-stage parasite antigens that were inoculated to immunize BALB/c mice. The presence of specific antibodies in vaccinated mice serum was studied by immunoblot analysis at different days after vaccination and showed an intensive immune response to a wide range of antigens with molecular weight ranging between 22 and 250 kDa. The humoral response allowed delay of the infection after the inoculation to high lethal doses of P. yoelii yoelii 17XL resulting in a partial protection against malaria disease, although final survival was managed in a low proportion of challenged mice. This approach shows the potential to prevent malaria disease with a set of antigens isolated from blood-stage parasites. |
| format | Article |
| id | doaj-art-45ad6ae17e7b401b9de4f301ab5a81ab |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-45ad6ae17e7b401b9de4f301ab5a81ab2025-02-03T06:08:17ZengWileyJournal of Immunology Research2314-88612314-71562015-01-01201510.1155/2015/723946723946Experimental Immunization Based on Plasmodium Antigens Isolated by Antibody AffinityAli N. Kamali0Patricia Marín-García1Isabel G. Azcárate2Antonio Puyet3Amalia Diez4José M. Bautista5Department of Biochemistry and Molecular Biology IV, Universidad Complutense de Madrid, Facultad de Veterinaria, Ciudad Universitaria, 28040 Madrid, SpainDepartment of Biochemistry and Molecular Biology IV, Universidad Complutense de Madrid, Facultad de Veterinaria, Ciudad Universitaria, 28040 Madrid, SpainDepartment of Biochemistry and Molecular Biology IV, Universidad Complutense de Madrid, Facultad de Veterinaria, Ciudad Universitaria, 28040 Madrid, SpainDepartment of Biochemistry and Molecular Biology IV, Universidad Complutense de Madrid, Facultad de Veterinaria, Ciudad Universitaria, 28040 Madrid, SpainDepartment of Biochemistry and Molecular Biology IV, Universidad Complutense de Madrid, Facultad de Veterinaria, Ciudad Universitaria, 28040 Madrid, SpainDepartment of Biochemistry and Molecular Biology IV, Universidad Complutense de Madrid, Facultad de Veterinaria, Ciudad Universitaria, 28040 Madrid, SpainVaccines blocking malaria parasites in the blood-stage diminish mortality and morbidity caused by the disease. Here, we isolated antigens from total parasite proteins by antibody affinity chromatography to test an immunization against lethal malaria infection in a murine model. We used the sera of malaria self-resistant ICR mice to lethal Plasmodium yoelii yoelii 17XL for purification of their IgGs which were subsequently employed to isolate blood-stage parasite antigens that were inoculated to immunize BALB/c mice. The presence of specific antibodies in vaccinated mice serum was studied by immunoblot analysis at different days after vaccination and showed an intensive immune response to a wide range of antigens with molecular weight ranging between 22 and 250 kDa. The humoral response allowed delay of the infection after the inoculation to high lethal doses of P. yoelii yoelii 17XL resulting in a partial protection against malaria disease, although final survival was managed in a low proportion of challenged mice. This approach shows the potential to prevent malaria disease with a set of antigens isolated from blood-stage parasites.http://dx.doi.org/10.1155/2015/723946 |
| spellingShingle | Ali N. Kamali Patricia Marín-García Isabel G. Azcárate Antonio Puyet Amalia Diez José M. Bautista Experimental Immunization Based on Plasmodium Antigens Isolated by Antibody Affinity Journal of Immunology Research |
| title | Experimental Immunization Based on Plasmodium Antigens Isolated by Antibody Affinity |
| title_full | Experimental Immunization Based on Plasmodium Antigens Isolated by Antibody Affinity |
| title_fullStr | Experimental Immunization Based on Plasmodium Antigens Isolated by Antibody Affinity |
| title_full_unstemmed | Experimental Immunization Based on Plasmodium Antigens Isolated by Antibody Affinity |
| title_short | Experimental Immunization Based on Plasmodium Antigens Isolated by Antibody Affinity |
| title_sort | experimental immunization based on plasmodium antigens isolated by antibody affinity |
| url | http://dx.doi.org/10.1155/2015/723946 |
| work_keys_str_mv | AT alinkamali experimentalimmunizationbasedonplasmodiumantigensisolatedbyantibodyaffinity AT patriciamaringarcia experimentalimmunizationbasedonplasmodiumantigensisolatedbyantibodyaffinity AT isabelgazcarate experimentalimmunizationbasedonplasmodiumantigensisolatedbyantibodyaffinity AT antoniopuyet experimentalimmunizationbasedonplasmodiumantigensisolatedbyantibodyaffinity AT amaliadiez experimentalimmunizationbasedonplasmodiumantigensisolatedbyantibodyaffinity AT josembautista experimentalimmunizationbasedonplasmodiumantigensisolatedbyantibodyaffinity |