Effects of Astaxanthin on the Physiological State of Porcine Ovarian Granulose Cells Cultured In Vitro
The physiological state of Granulosa cells (GCs) is intricately linked to the growth and development of oocytes. Oxidative stress has been found to cause damage to GCs in vitro. Astaxanthin (AST), a well-known natural ketone-type carotenoid, has demonstrated strong antioxidant properties. This study...
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MDPI AG
2024-09-01
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| Series: | Antioxidants |
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| author | Xiaofen Yang Dongping Zhou Lv Gao Yanxin Wang Yun Wang Ruru Jia Yuwei Bai Deshun Shi Fenghua Lu |
| author_facet | Xiaofen Yang Dongping Zhou Lv Gao Yanxin Wang Yun Wang Ruru Jia Yuwei Bai Deshun Shi Fenghua Lu |
| author_sort | Xiaofen Yang |
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| description | The physiological state of Granulosa cells (GCs) is intricately linked to the growth and development of oocytes. Oxidative stress has been found to cause damage to GCs in vitro. Astaxanthin (AST), a well-known natural ketone-type carotenoid, has demonstrated strong antioxidant properties. This study investigates the impact of astaxanthin supplementation on the physiological state of porcine ovarian granulosa cells cultured in vitro. Variations in morphology, apoptosis, reactive oxygen species (ROS) levels, and the expression of apoptosis and anti-oxidation-related genes in porcine GCs from different passages were observed. Significant morphological changes, increases in apoptosis, and decreases in antioxidant capacity resulting from passage were observed. Subsequently, treatment with 5 μmol/L astaxanthin significantly enhanced cell viability, proliferation, antioxidant capacity and mitochondrial function while also regulating the estradiol (E2) and progesterone (P4) levels. Additionally, the gene expression of antioxidation, E2, and P4 synthesis markers was assessed, revealing reduced apoptosis and ROS levels in porcine GCs. In conclusion, supplementation with 5 μmol/L astaxanthin in vitro effectively enhances the physiological condition of porcine GCs and optimizes the culture system for these cells in vitro. Optimizing the culture system of porcine GCs in vitro can simulate the function of granulosa cells in vivo and provide a theoretical reference for further promoting follicular development, which is beneficial to improving sow fertility in actual production. |
| format | Article |
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| institution | OA Journals |
| issn | 2076-3921 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | MDPI AG |
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| series | Antioxidants |
| spelling | doaj-art-450c9e023a2a4e0fafbded0fc0d23e112025-08-20T02:11:08ZengMDPI AGAntioxidants2076-39212024-09-011310118510.3390/antiox13101185Effects of Astaxanthin on the Physiological State of Porcine Ovarian Granulose Cells Cultured In VitroXiaofen Yang0Dongping Zhou1Lv Gao2Yanxin Wang3Yun Wang4Ruru Jia5Yuwei Bai6Deshun Shi7Fenghua Lu8Guangxi Key Laboratory of Animal Breeding & Disease Control and Prevention, Guangxi University, 75 Xiuling Road, Nanning 530005, ChinaGuangxi Key Laboratory of Animal Breeding & Disease Control and Prevention, Guangxi University, 75 Xiuling Road, Nanning 530005, ChinaGuangxi Key Laboratory of Animal Breeding & Disease Control and Prevention, Guangxi University, 75 Xiuling Road, Nanning 530005, ChinaGuangxi Key Laboratory of Animal Breeding & Disease Control and Prevention, Guangxi University, 75 Xiuling Road, Nanning 530005, ChinaGuangxi Key Laboratory of Animal Breeding & Disease Control and Prevention, Guangxi University, 75 Xiuling Road, Nanning 530005, ChinaGuangxi Key Laboratory of Animal Breeding & Disease Control and Prevention, Guangxi University, 75 Xiuling Road, Nanning 530005, ChinaGuangxi Key Laboratory of Animal Breeding & Disease Control and Prevention, Guangxi University, 75 Xiuling Road, Nanning 530005, ChinaGuangxi Key Laboratory of Animal Breeding & Disease Control and Prevention, Guangxi University, 75 Xiuling Road, Nanning 530005, ChinaGuangxi Key Laboratory of Animal Breeding & Disease Control and Prevention, Guangxi University, 75 Xiuling Road, Nanning 530005, ChinaThe physiological state of Granulosa cells (GCs) is intricately linked to the growth and development of oocytes. Oxidative stress has been found to cause damage to GCs in vitro. Astaxanthin (AST), a well-known natural ketone-type carotenoid, has demonstrated strong antioxidant properties. This study investigates the impact of astaxanthin supplementation on the physiological state of porcine ovarian granulosa cells cultured in vitro. Variations in morphology, apoptosis, reactive oxygen species (ROS) levels, and the expression of apoptosis and anti-oxidation-related genes in porcine GCs from different passages were observed. Significant morphological changes, increases in apoptosis, and decreases in antioxidant capacity resulting from passage were observed. Subsequently, treatment with 5 μmol/L astaxanthin significantly enhanced cell viability, proliferation, antioxidant capacity and mitochondrial function while also regulating the estradiol (E2) and progesterone (P4) levels. Additionally, the gene expression of antioxidation, E2, and P4 synthesis markers was assessed, revealing reduced apoptosis and ROS levels in porcine GCs. In conclusion, supplementation with 5 μmol/L astaxanthin in vitro effectively enhances the physiological condition of porcine GCs and optimizes the culture system for these cells in vitro. Optimizing the culture system of porcine GCs in vitro can simulate the function of granulosa cells in vivo and provide a theoretical reference for further promoting follicular development, which is beneficial to improving sow fertility in actual production.https://www.mdpi.com/2076-3921/13/10/1185porcine ovarian granulosa cellsastaxanthinin vitrophysiological state |
| spellingShingle | Xiaofen Yang Dongping Zhou Lv Gao Yanxin Wang Yun Wang Ruru Jia Yuwei Bai Deshun Shi Fenghua Lu Effects of Astaxanthin on the Physiological State of Porcine Ovarian Granulose Cells Cultured In Vitro Antioxidants porcine ovarian granulosa cells astaxanthin in vitro physiological state |
| title | Effects of Astaxanthin on the Physiological State of Porcine Ovarian Granulose Cells Cultured In Vitro |
| title_full | Effects of Astaxanthin on the Physiological State of Porcine Ovarian Granulose Cells Cultured In Vitro |
| title_fullStr | Effects of Astaxanthin on the Physiological State of Porcine Ovarian Granulose Cells Cultured In Vitro |
| title_full_unstemmed | Effects of Astaxanthin on the Physiological State of Porcine Ovarian Granulose Cells Cultured In Vitro |
| title_short | Effects of Astaxanthin on the Physiological State of Porcine Ovarian Granulose Cells Cultured In Vitro |
| title_sort | effects of astaxanthin on the physiological state of porcine ovarian granulose cells cultured in vitro |
| topic | porcine ovarian granulosa cells astaxanthin in vitro physiological state |
| url | https://www.mdpi.com/2076-3921/13/10/1185 |
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