Construction of functional tissue-engineered microvasculatures using circulating fibrocytes as mural cells
Mural cells are essential for maintaining the proper functions of microvasculatures. However, a key challenge of microvascular tissue engineering is identifying a cellular source for mural cells. We showed that in vitro , circulating fibrocytes (CFs) can (1) shear and stabilize the microvasculatures...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2025-01-01
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| Series: | Journal of Tissue Engineering |
| Online Access: | https://doi.org/10.1177/20417314251315523 |
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| _version_ | 1832583293467361280 |
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| author | Xiaolin Wang Hong Tan Harsha P Gunawardena Jin Cao Lu Dang Hongbing Deng Xueyong Li Hongjun Wang Jinqing Li Caibin Cui David A Gerber |
| author_facet | Xiaolin Wang Hong Tan Harsha P Gunawardena Jin Cao Lu Dang Hongbing Deng Xueyong Li Hongjun Wang Jinqing Li Caibin Cui David A Gerber |
| author_sort | Xiaolin Wang |
| collection | DOAJ |
| description | Mural cells are essential for maintaining the proper functions of microvasculatures. However, a key challenge of microvascular tissue engineering is identifying a cellular source for mural cells. We showed that in vitro , circulating fibrocytes (CFs) can (1) shear and stabilize the microvasculatures formed by vascular endothelial cells (VECs) in a collagen gel, (2) form gap junctions with VECs and (3) induce basement membrane formation. CFs transplanted into nude mice along with VECs in either collagen gel or Matrigel exhibited activities similar to those mentioned above, that is, sheathing microvasculatures formed by VECs, inducing basement membrane formation and facilitating the connection of the engineered microvasculatures with the host circulation. Interestingly, the behaviour of CFs also differs from that of human brain vascular pericytes (HBVPs) in vitro , which often infiltrate the lumen of capillary-like structures in a mosaic pattern, actively proliferate and exhibit lower endocytosis and migration capacities. We concluded that CFs are a suitable cellular source for mural cells in the construction of tissue-engineered microvasculatures. |
| format | Article |
| id | doaj-art-44cf3c3143fc45ccbb1d6d9780ae250d |
| institution | Kabale University |
| issn | 2041-7314 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Tissue Engineering |
| spelling | doaj-art-44cf3c3143fc45ccbb1d6d9780ae250d2025-01-28T17:03:20ZengSAGE PublishingJournal of Tissue Engineering2041-73142025-01-011610.1177/20417314251315523Construction of functional tissue-engineered microvasculatures using circulating fibrocytes as mural cellsXiaolin Wang0Hong Tan1Harsha P Gunawardena2Jin Cao3Lu Dang4Hongbing Deng5Xueyong Li6Hongjun Wang7Jinqing Li8Caibin Cui9David A Gerber10Department of Burn/Plastic Surgery and Wound Repair, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, ChinaNorthwest University School of Medicine, Xi’an, ChinaJanssen Research and Development LLC, Janssen Pharmaceutical Companies of Johnson and Johnson, Spring House, PA, USADepartment of Burn/Plastic Surgery and Wound Repair, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, ChinaDepartment of Burn/Plastic Surgery and Wound Repair, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, ChinaDepartment of Environmental Science, School of Resource and Environmental Science, Wuhan University, Wuhan, ChinaDepartment of Burn/Plastic Surgery and Wound Repair, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, ChinaBiomedical Engineering, Chemistry and Biological Sciences, Stevens Institute of Technology, Hoboken, NJ, USADepartment of Burn/Plastic Surgery and Wound Repair, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, ChinaDepartment of Surgery, University of North Carolina at Chapel Hill, Chapel Hill, NC, USADepartment of Surgery, University of North Carolina at Chapel Hill, Chapel Hill, NC, USAMural cells are essential for maintaining the proper functions of microvasculatures. However, a key challenge of microvascular tissue engineering is identifying a cellular source for mural cells. We showed that in vitro , circulating fibrocytes (CFs) can (1) shear and stabilize the microvasculatures formed by vascular endothelial cells (VECs) in a collagen gel, (2) form gap junctions with VECs and (3) induce basement membrane formation. CFs transplanted into nude mice along with VECs in either collagen gel or Matrigel exhibited activities similar to those mentioned above, that is, sheathing microvasculatures formed by VECs, inducing basement membrane formation and facilitating the connection of the engineered microvasculatures with the host circulation. Interestingly, the behaviour of CFs also differs from that of human brain vascular pericytes (HBVPs) in vitro , which often infiltrate the lumen of capillary-like structures in a mosaic pattern, actively proliferate and exhibit lower endocytosis and migration capacities. We concluded that CFs are a suitable cellular source for mural cells in the construction of tissue-engineered microvasculatures.https://doi.org/10.1177/20417314251315523 |
| spellingShingle | Xiaolin Wang Hong Tan Harsha P Gunawardena Jin Cao Lu Dang Hongbing Deng Xueyong Li Hongjun Wang Jinqing Li Caibin Cui David A Gerber Construction of functional tissue-engineered microvasculatures using circulating fibrocytes as mural cells Journal of Tissue Engineering |
| title | Construction of functional tissue-engineered microvasculatures using circulating fibrocytes as mural cells |
| title_full | Construction of functional tissue-engineered microvasculatures using circulating fibrocytes as mural cells |
| title_fullStr | Construction of functional tissue-engineered microvasculatures using circulating fibrocytes as mural cells |
| title_full_unstemmed | Construction of functional tissue-engineered microvasculatures using circulating fibrocytes as mural cells |
| title_short | Construction of functional tissue-engineered microvasculatures using circulating fibrocytes as mural cells |
| title_sort | construction of functional tissue engineered microvasculatures using circulating fibrocytes as mural cells |
| url | https://doi.org/10.1177/20417314251315523 |
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