Preparation of Bioactive Titanium Surfaces via Fluoride and Fibronectin Retention
Statement of Problem. The chemical or topographic modification of the dental implant surface can affect bone healing, promote accelerated osteogenesis, and increase bone-implant contact and bonding strength. Objective. In this work, the effects of dental implant surface treatment and fibronectin ads...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | International Journal of Biomaterials |
| Online Access: | http://dx.doi.org/10.1155/2012/290179 |
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| _version_ | 1832565708056166400 |
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| author | Carlos Nelson Elias Patricia Abdo Gravina Costa e Silva Filho Pedro Augusto de Paula Nascente |
| author_facet | Carlos Nelson Elias Patricia Abdo Gravina Costa e Silva Filho Pedro Augusto de Paula Nascente |
| author_sort | Carlos Nelson Elias |
| collection | DOAJ |
| description | Statement of Problem. The chemical or topographic modification of the dental implant surface can affect bone healing, promote accelerated osteogenesis, and increase bone-implant contact and bonding strength. Objective. In this work, the effects of dental implant surface treatment and fibronectin adsorption on the adhesion of osteoblasts were analyzed. Materials and Methods. Two titanium dental implants (Porous-acid etching and PorousNano-acid etching followed by fluoride ion modification) were characterized by high-resolution scanning electron microscopy, atomic force microscopy, and X-ray diffraction before and after the incorporation of human plasma fibronectin (FN). The objective was to investigate the biofunctionalization of these surfaces and examine their effects on the interaction with osteoblastic cells. Results. The evaluation techniques used showed that the Porous and PorousNano implants have similar microstructural characteristics. Spectrophotometry demonstrated similar levels of fibronectin adsorption on both surfaces (80%). The association indexes of osteoblastic cells in FN-treated samples were significantly higher than those in samples without FN. The radioactivity values associated with the same samples, expressed as counts per minute (cpm), suggested that FN incorporation is an important determinant of the in vitro cytocompatibility of the surfaces. Conclusion. The preparation of bioactive titanium surfaces via fluoride and FN retention proved to be a useful treatment to optimize and to accelerate the osseointegration process for dental implants. |
| format | Article |
| id | doaj-art-42edbbd7fdf34300ac62b8f9ad3f18a3 |
| institution | Kabale University |
| issn | 1687-8787 1687-8795 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Biomaterials |
| spelling | doaj-art-42edbbd7fdf34300ac62b8f9ad3f18a32025-02-03T01:06:47ZengWileyInternational Journal of Biomaterials1687-87871687-87952012-01-01201210.1155/2012/290179290179Preparation of Bioactive Titanium Surfaces via Fluoride and Fibronectin RetentionCarlos Nelson Elias0Patricia Abdo Gravina1Costa e Silva Filho2Pedro Augusto de Paula Nascente3Biomaterials Laboratory, Instituto Militar de Engenharia, Pr Gen Tibúrcio 80, 22290-270 Rio de Janeiro, RJ, BrazilBiomaterials Laboratory, Instituto Militar de Engenharia, Pr Gen Tibúrcio 80, 22290-270 Rio de Janeiro, RJ, BrazilAvenue Carlos Chagas Filho, 373, Cidade Universitária-21941-902 Rio de janeiro, RJ, BrazilRodovia Washington Luís km 235, 13565-905 São Carlos, SP, BrazilStatement of Problem. The chemical or topographic modification of the dental implant surface can affect bone healing, promote accelerated osteogenesis, and increase bone-implant contact and bonding strength. Objective. In this work, the effects of dental implant surface treatment and fibronectin adsorption on the adhesion of osteoblasts were analyzed. Materials and Methods. Two titanium dental implants (Porous-acid etching and PorousNano-acid etching followed by fluoride ion modification) were characterized by high-resolution scanning electron microscopy, atomic force microscopy, and X-ray diffraction before and after the incorporation of human plasma fibronectin (FN). The objective was to investigate the biofunctionalization of these surfaces and examine their effects on the interaction with osteoblastic cells. Results. The evaluation techniques used showed that the Porous and PorousNano implants have similar microstructural characteristics. Spectrophotometry demonstrated similar levels of fibronectin adsorption on both surfaces (80%). The association indexes of osteoblastic cells in FN-treated samples were significantly higher than those in samples without FN. The radioactivity values associated with the same samples, expressed as counts per minute (cpm), suggested that FN incorporation is an important determinant of the in vitro cytocompatibility of the surfaces. Conclusion. The preparation of bioactive titanium surfaces via fluoride and FN retention proved to be a useful treatment to optimize and to accelerate the osseointegration process for dental implants.http://dx.doi.org/10.1155/2012/290179 |
| spellingShingle | Carlos Nelson Elias Patricia Abdo Gravina Costa e Silva Filho Pedro Augusto de Paula Nascente Preparation of Bioactive Titanium Surfaces via Fluoride and Fibronectin Retention International Journal of Biomaterials |
| title | Preparation of Bioactive Titanium Surfaces via Fluoride and Fibronectin Retention |
| title_full | Preparation of Bioactive Titanium Surfaces via Fluoride and Fibronectin Retention |
| title_fullStr | Preparation of Bioactive Titanium Surfaces via Fluoride and Fibronectin Retention |
| title_full_unstemmed | Preparation of Bioactive Titanium Surfaces via Fluoride and Fibronectin Retention |
| title_short | Preparation of Bioactive Titanium Surfaces via Fluoride and Fibronectin Retention |
| title_sort | preparation of bioactive titanium surfaces via fluoride and fibronectin retention |
| url | http://dx.doi.org/10.1155/2012/290179 |
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