Modulation of Human Colostrum Phagocyte Activity by the Glycine-Adsorbed Polyethylene Glycol Microspheres
Colostrum is a secretion that contains immunologically active components, including immunocompetent cells and glycine, which has anti-inflammatory, immunomodulatory, and cytoprotective effects. The aim of this study was to evaluate the adsorption of glycine onto polyethylene glycol (PEG) microsphere...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/845270 |
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| author | Paulo Celso Leventi Guimarães Adenilda Cristina Honorio-França Cristiane de Castro Pernet Hara Danny Laura Gomes Fagundes Silvia Hanna Bilotti Ratto Eduardo Luzía França |
| author_facet | Paulo Celso Leventi Guimarães Adenilda Cristina Honorio-França Cristiane de Castro Pernet Hara Danny Laura Gomes Fagundes Silvia Hanna Bilotti Ratto Eduardo Luzía França |
| author_sort | Paulo Celso Leventi Guimarães |
| collection | DOAJ |
| description | Colostrum is a secretion that contains immunologically active components, including immunocompetent cells and glycine, which has anti-inflammatory, immunomodulatory, and cytoprotective effects. The aim of this study was to evaluate the adsorption of glycine onto polyethylene glycol (PEG) microspheres and to verify the immunomodulatory effect of this nanomaterial on human colostrum phagocytes. The PEG microspheres were evaluated by fluorescence microscopy. The effects of PEG microspheres with adsorbed glycine on viability, superoxide release, phagocytosis, microbicidal activity, and intracellular calcium release of mononuclear (MN) and polymorphonuclear (PMN) colostrum phagocytes were determined. Fluorescence microscopy analyses revealed that glycine was able to be adsorbed to the PEG microspheres. The PMN phagocytes exposed to glycine-PEG microspheres showed the highest superoxide levels. The phagocytes (both MN and PMN) displayed increased microbicidal activity and intracellular calcium release in the presence of PEG microspheres with adsorbed glycine. These data suggest that the adsorption of PEG microspheres with adsorbed glycine was able to stimulate the colostrum phagocytes. This material may represent a possible alternative therapy for future clinical applications on patients with gastrointestinal infections. |
| format | Article |
| id | doaj-art-4289e64cfad74f7ebba8b9bf4c8e5e3d |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-4289e64cfad74f7ebba8b9bf4c8e5e3d2025-02-03T01:09:05ZengWileyJournal of Chemistry2090-90632090-90712013-01-01201310.1155/2013/845270845270Modulation of Human Colostrum Phagocyte Activity by the Glycine-Adsorbed Polyethylene Glycol MicrospheresPaulo Celso Leventi Guimarães0Adenilda Cristina Honorio-França1Cristiane de Castro Pernet Hara2Danny Laura Gomes Fagundes3Silvia Hanna Bilotti Ratto4Eduardo Luzía França5Institute of Biological and Health Science, Federal University of Mato Grosso (UFMT), Rodovia Br 070, Km 5 s/no, 78600-000 Barra do Garças, MT, BrazilInstitute of Biological and Health Science, Federal University of Mato Grosso (UFMT), Rodovia Br 070, Km 5 s/no, 78600-000 Barra do Garças, MT, BrazilInstitute of Biological and Health Science, Federal University of Mato Grosso (UFMT), Rodovia Br 070, Km 5 s/no, 78600-000 Barra do Garças, MT, BrazilInstitute of Biological and Health Science, Federal University of Mato Grosso (UFMT), Rodovia Br 070, Km 5 s/no, 78600-000 Barra do Garças, MT, BrazilInstitute of Biological and Health Science, Federal University of Mato Grosso (UFMT), Rodovia Br 070, Km 5 s/no, 78600-000 Barra do Garças, MT, BrazilInstitute of Biological and Health Science, Federal University of Mato Grosso (UFMT), Rodovia Br 070, Km 5 s/no, 78600-000 Barra do Garças, MT, BrazilColostrum is a secretion that contains immunologically active components, including immunocompetent cells and glycine, which has anti-inflammatory, immunomodulatory, and cytoprotective effects. The aim of this study was to evaluate the adsorption of glycine onto polyethylene glycol (PEG) microspheres and to verify the immunomodulatory effect of this nanomaterial on human colostrum phagocytes. The PEG microspheres were evaluated by fluorescence microscopy. The effects of PEG microspheres with adsorbed glycine on viability, superoxide release, phagocytosis, microbicidal activity, and intracellular calcium release of mononuclear (MN) and polymorphonuclear (PMN) colostrum phagocytes were determined. Fluorescence microscopy analyses revealed that glycine was able to be adsorbed to the PEG microspheres. The PMN phagocytes exposed to glycine-PEG microspheres showed the highest superoxide levels. The phagocytes (both MN and PMN) displayed increased microbicidal activity and intracellular calcium release in the presence of PEG microspheres with adsorbed glycine. These data suggest that the adsorption of PEG microspheres with adsorbed glycine was able to stimulate the colostrum phagocytes. This material may represent a possible alternative therapy for future clinical applications on patients with gastrointestinal infections.http://dx.doi.org/10.1155/2013/845270 |
| spellingShingle | Paulo Celso Leventi Guimarães Adenilda Cristina Honorio-França Cristiane de Castro Pernet Hara Danny Laura Gomes Fagundes Silvia Hanna Bilotti Ratto Eduardo Luzía França Modulation of Human Colostrum Phagocyte Activity by the Glycine-Adsorbed Polyethylene Glycol Microspheres Journal of Chemistry |
| title | Modulation of Human Colostrum Phagocyte Activity by the Glycine-Adsorbed Polyethylene Glycol Microspheres |
| title_full | Modulation of Human Colostrum Phagocyte Activity by the Glycine-Adsorbed Polyethylene Glycol Microspheres |
| title_fullStr | Modulation of Human Colostrum Phagocyte Activity by the Glycine-Adsorbed Polyethylene Glycol Microspheres |
| title_full_unstemmed | Modulation of Human Colostrum Phagocyte Activity by the Glycine-Adsorbed Polyethylene Glycol Microspheres |
| title_short | Modulation of Human Colostrum Phagocyte Activity by the Glycine-Adsorbed Polyethylene Glycol Microspheres |
| title_sort | modulation of human colostrum phagocyte activity by the glycine adsorbed polyethylene glycol microspheres |
| url | http://dx.doi.org/10.1155/2013/845270 |
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