Blastocystis and Giardia duodenalis infection in a male prison in Spain
Background: General conditions in a prison may facilitate water- or food-borne infections. Methods: Detection of intestinal parasites was achieved in 471 male prison inmates by standard microscopic procedures on their stool samples. Positive samples were processed by PCR amplification of a 600-bp fr...
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Elsevier
2025-02-01
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| Series: | Parasite Epidemiology and Control |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405673124000710 |
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| author | Carla Muñoz-Antoli Jacklyn Comas María José Irisarri-Gutiérrez Lucrecia Acosta José Guillermo Esteban Rafael Toledo |
| author_facet | Carla Muñoz-Antoli Jacklyn Comas María José Irisarri-Gutiérrez Lucrecia Acosta José Guillermo Esteban Rafael Toledo |
| author_sort | Carla Muñoz-Antoli |
| collection | DOAJ |
| description | Background: General conditions in a prison may facilitate water- or food-borne infections. Methods: Detection of intestinal parasites was achieved in 471 male prison inmates by standard microscopic procedures on their stool samples. Positive samples were processed by PCR amplification of a 600-bp fragment of the Blastocystis SSU rRNA gene and partial sequences of the Giardia duodenalis bg genes. Identification of subtypes/genotypes was based on Sanger sequencing methods. Results: Blastocystis was found in 7.9 % (37/471) and G. duodenalis was found in 2.1 % (10/471). Out of the 37 Blastocystis positive samples, 54 % (20/37) were successfully subtyped, allowing the identification of the subtypes ST3 (50 %), ST1 (25 %), ST2 (15 %), ST4 (5 %) and ST6 (5 %). Out of 10 G. duodenalis positive samples, 50 % (5/10) were successfully genotyped, allowing the identification of genotypes A (80 %) and B (20 %). Conclusions: The predominance of ST3 within the prison inmates, together with its low intra-ST genetic variability, reflected inter-human transmission with spatial stability. The G. duodenalis distribution is not wide enough to consider the possibility of a generalized transmission via contaminated water or food. Personal hygiene practices among male prison inmates may be an important measure to prevent the transmission. |
| format | Article |
| id | doaj-art-42815af8797b40beaf09c103741ba0bb |
| institution | Kabale University |
| issn | 2405-6731 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Parasite Epidemiology and Control |
| spelling | doaj-art-42815af8797b40beaf09c103741ba0bb2025-02-06T05:12:30ZengElsevierParasite Epidemiology and Control2405-67312025-02-0128e00407Blastocystis and Giardia duodenalis infection in a male prison in SpainCarla Muñoz-Antoli0Jacklyn Comas1María José Irisarri-Gutiérrez2Lucrecia Acosta3José Guillermo Esteban4Rafael Toledo5Department of Pharmacy and Pharmaceutical Technology and Parasitology, Faculty of Pharmacy, University of Valencia, Valencia, Spain; Corresponding author at: Área Parasitología, Departamento Farmacia y Tecnología Farmacéutica y Parasitología, Facultad Farmacia, Universidad Valencia, Valencia, Spain.Department of Pharmacy and Pharmaceutical Technology and Parasitology, Faculty of Pharmacy, University of Valencia, Valencia, Spain; Grupo de Investigación Salud y Comunidad, Línea de Enfermedades Infecciosas Tropicales, Universidad Tecnológica del Chocó Diego Luis Córdoba, Chocó, ColombiaDepartment of Preventive Medicine, Public Health and Microbiology, Faculty of Medicine, Autonomous University of Madrid, Madrid, SpainParasitology Area, Department of Agrochemistry and Environment, Miguel Hernández de Elche University, Alicante, SpainDepartment of Pharmacy and Pharmaceutical Technology and Parasitology, Faculty of Pharmacy, University of Valencia, Valencia, SpainDepartment of Pharmacy and Pharmaceutical Technology and Parasitology, Faculty of Pharmacy, University of Valencia, Valencia, SpainBackground: General conditions in a prison may facilitate water- or food-borne infections. Methods: Detection of intestinal parasites was achieved in 471 male prison inmates by standard microscopic procedures on their stool samples. Positive samples were processed by PCR amplification of a 600-bp fragment of the Blastocystis SSU rRNA gene and partial sequences of the Giardia duodenalis bg genes. Identification of subtypes/genotypes was based on Sanger sequencing methods. Results: Blastocystis was found in 7.9 % (37/471) and G. duodenalis was found in 2.1 % (10/471). Out of the 37 Blastocystis positive samples, 54 % (20/37) were successfully subtyped, allowing the identification of the subtypes ST3 (50 %), ST1 (25 %), ST2 (15 %), ST4 (5 %) and ST6 (5 %). Out of 10 G. duodenalis positive samples, 50 % (5/10) were successfully genotyped, allowing the identification of genotypes A (80 %) and B (20 %). Conclusions: The predominance of ST3 within the prison inmates, together with its low intra-ST genetic variability, reflected inter-human transmission with spatial stability. The G. duodenalis distribution is not wide enough to consider the possibility of a generalized transmission via contaminated water or food. Personal hygiene practices among male prison inmates may be an important measure to prevent the transmission.http://www.sciencedirect.com/science/article/pii/S2405673124000710BlastocystisGiardia duodenalisHuman infectionPrisonInmatesSpain |
| spellingShingle | Carla Muñoz-Antoli Jacklyn Comas María José Irisarri-Gutiérrez Lucrecia Acosta José Guillermo Esteban Rafael Toledo Blastocystis and Giardia duodenalis infection in a male prison in Spain Parasite Epidemiology and Control Blastocystis Giardia duodenalis Human infection Prison Inmates Spain |
| title | Blastocystis and Giardia duodenalis infection in a male prison in Spain |
| title_full | Blastocystis and Giardia duodenalis infection in a male prison in Spain |
| title_fullStr | Blastocystis and Giardia duodenalis infection in a male prison in Spain |
| title_full_unstemmed | Blastocystis and Giardia duodenalis infection in a male prison in Spain |
| title_short | Blastocystis and Giardia duodenalis infection in a male prison in Spain |
| title_sort | blastocystis and giardia duodenalis infection in a male prison in spain |
| topic | Blastocystis Giardia duodenalis Human infection Prison Inmates Spain |
| url | http://www.sciencedirect.com/science/article/pii/S2405673124000710 |
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