Combined Administration of Streptozotocin and Sucrose Accelerates the Appearance of Type 2 Diabetes Symptoms in Rats
Type 2 diabetes is a disease with a high global prevalence, characterized by chronic hyperglycemia, insulin resistance, polyphagia, polydipsia, polyuria, and changes in body weight. Animal models have been very useful for the study of this disease and to search for new therapeutic targets that delay...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2019/3791061 |
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| author | Martha Isela Barragán-Bonilla Juan Miguel Mendoza-Bello Penélope Aguilera Isela Parra-Rojas Berenice Illades-Aguiar Mónica Ramírez Mónica Espinoza-Rojo |
| author_facet | Martha Isela Barragán-Bonilla Juan Miguel Mendoza-Bello Penélope Aguilera Isela Parra-Rojas Berenice Illades-Aguiar Mónica Ramírez Mónica Espinoza-Rojo |
| author_sort | Martha Isela Barragán-Bonilla |
| collection | DOAJ |
| description | Type 2 diabetes is a disease with a high global prevalence, characterized by chronic hyperglycemia, insulin resistance, polyphagia, polydipsia, polyuria, and changes in body weight. Animal models have been very useful for the study of this disease and to search for new therapeutic targets that delay, attenuate, or avoid diabetic complications. The purpose of this work was to establish a model of type 2 diabetes and exhibit the majority of the characteristics of the disease. Two-day-old male and female Wistar rats were treated once with streptozotocin (70 or 90 mg/kg body weight). After weaning, they were given a sucrose-sweetened beverage (SSB; sucrose at 10 or 30%) during 7 or 11 weeks; their body weight and food intake were measured daily. With the rats at 14 weeks of age, we determined the following: (a) fasting blood glucose, (b) oral glucose tolerance, and (c) insulin tolerance. We found that the supplementation of sucrose at 10% for 7 weeks in male rats which had previously been given streptozotocin (70 mg/kg) at neonatal stage leads to the appearance of the signs and symptoms of the characteristic of type 2 diabetes in adulthood. |
| format | Article |
| id | doaj-art-4247a3c58e3442ea8d9f6d0dbda2752d |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-4247a3c58e3442ea8d9f6d0dbda2752d2025-02-03T05:57:27ZengWileyJournal of Diabetes Research2314-67452314-67532019-01-01201910.1155/2019/37910613791061Combined Administration of Streptozotocin and Sucrose Accelerates the Appearance of Type 2 Diabetes Symptoms in RatsMartha Isela Barragán-Bonilla0Juan Miguel Mendoza-Bello1Penélope Aguilera2Isela Parra-Rojas3Berenice Illades-Aguiar4Mónica Ramírez5Mónica Espinoza-Rojo6Laboratorio de Biología Molecular y Genómica de la Facultad de Ciencias Químico-Biológicas, Universidad Autónoma de Guerrero, Av. Lázaro Cárdenas S/N, Ciudad Universitaria, Chilpancingo de los Bravo, Gro. 39090, MexicoLaboratorio de Biología Molecular y Genómica de la Facultad de Ciencias Químico-Biológicas, Universidad Autónoma de Guerrero, Av. Lázaro Cárdenas S/N, Ciudad Universitaria, Chilpancingo de los Bravo, Gro. 39090, MexicoLaboratorio de Patología Vascular Cerebral, Instituto Nacional de Neurología y Neurocirugía “Manuel Velasco Suárez”, Av. Insurgentes Sur 3877, Mexico City 14269, MexicoLaboratorio de Obesidad y Diabetes de la Facultad de Ciencias Químico-Biológicas, Universidad Autónoma de Guerrero, Av. Lázaro Cárdenas S/N Ciudad Universitaria, Chilpancingo de los Bravo, Gro. 39090, MexicoLaboratorio de Biomedicina Molecular de la Facultad de Ciencias Químico-Biológicas, Universidad Autónoma de Guerrero, Av. Lázaro Cárdenas S/N, Ciudad Universitaria, Chilpancingo de los Bravo, Gro. 39090, MexicoCONACYT-Universidad Autónoma de Guerrero, Av. Javier Méndez Aponte No. 1, Fracc. Servidor Agrario, Chilpancingo de los Bravo, Gro. 39070, MexicoLaboratorio de Biología Molecular y Genómica de la Facultad de Ciencias Químico-Biológicas, Universidad Autónoma de Guerrero, Av. Lázaro Cárdenas S/N, Ciudad Universitaria, Chilpancingo de los Bravo, Gro. 39090, MexicoType 2 diabetes is a disease with a high global prevalence, characterized by chronic hyperglycemia, insulin resistance, polyphagia, polydipsia, polyuria, and changes in body weight. Animal models have been very useful for the study of this disease and to search for new therapeutic targets that delay, attenuate, or avoid diabetic complications. The purpose of this work was to establish a model of type 2 diabetes and exhibit the majority of the characteristics of the disease. Two-day-old male and female Wistar rats were treated once with streptozotocin (70 or 90 mg/kg body weight). After weaning, they were given a sucrose-sweetened beverage (SSB; sucrose at 10 or 30%) during 7 or 11 weeks; their body weight and food intake were measured daily. With the rats at 14 weeks of age, we determined the following: (a) fasting blood glucose, (b) oral glucose tolerance, and (c) insulin tolerance. We found that the supplementation of sucrose at 10% for 7 weeks in male rats which had previously been given streptozotocin (70 mg/kg) at neonatal stage leads to the appearance of the signs and symptoms of the characteristic of type 2 diabetes in adulthood.http://dx.doi.org/10.1155/2019/3791061 |
| spellingShingle | Martha Isela Barragán-Bonilla Juan Miguel Mendoza-Bello Penélope Aguilera Isela Parra-Rojas Berenice Illades-Aguiar Mónica Ramírez Mónica Espinoza-Rojo Combined Administration of Streptozotocin and Sucrose Accelerates the Appearance of Type 2 Diabetes Symptoms in Rats Journal of Diabetes Research |
| title | Combined Administration of Streptozotocin and Sucrose Accelerates the Appearance of Type 2 Diabetes Symptoms in Rats |
| title_full | Combined Administration of Streptozotocin and Sucrose Accelerates the Appearance of Type 2 Diabetes Symptoms in Rats |
| title_fullStr | Combined Administration of Streptozotocin and Sucrose Accelerates the Appearance of Type 2 Diabetes Symptoms in Rats |
| title_full_unstemmed | Combined Administration of Streptozotocin and Sucrose Accelerates the Appearance of Type 2 Diabetes Symptoms in Rats |
| title_short | Combined Administration of Streptozotocin and Sucrose Accelerates the Appearance of Type 2 Diabetes Symptoms in Rats |
| title_sort | combined administration of streptozotocin and sucrose accelerates the appearance of type 2 diabetes symptoms in rats |
| url | http://dx.doi.org/10.1155/2019/3791061 |
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