Ultrasound-Assisted Enzymatic Extraction of the Active Components from <i>Acanthopanax sessiliflorus</i> Stem and Bioactivity Comparison with <i>Acanthopanax senticosus</i>
<i>Acanthopanax senticosus</i> (ASC) contains a variety of bioactive compounds and serves as an important traditional Chinese medicinal resource. However, its prolonged growth cycle and reliance on wild populations limit its practical use. To explore the potential of <i>Acanthopana...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/30/2/397 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832587793671389184 |
|---|---|
| author | Qiaomu You Yanjun Ren Jiaotong Li Guangnian Zeng Xiongfei Luo Chunying Zheng Zhonghua Tang |
| author_facet | Qiaomu You Yanjun Ren Jiaotong Li Guangnian Zeng Xiongfei Luo Chunying Zheng Zhonghua Tang |
| author_sort | Qiaomu You |
| collection | DOAJ |
| description | <i>Acanthopanax senticosus</i> (ASC) contains a variety of bioactive compounds and serves as an important traditional Chinese medicinal resource. However, its prolonged growth cycle and reliance on wild populations limit its practical use. To explore the potential of <i>Acanthopanax sessiliflorus</i> (ASF) as an alternative, this study focused on optimizing the extraction process and assessing the bioactivity of stem extracts. The Analytic Hierarchy Process-Entropy Weight Method (AHP-EWM) was applied to comprehensively score five key active compounds in ASF stems, establishing a benchmark for evaluating extraction efficiency. Ultrasound-assisted enzymatic extraction (UAEE) was employed, and orthogonal and response surface experiments were conducted to refine the extraction parameters. The optimal conditions identified were an ultrasound temperature of 59 °C, a duration of 57 min, an ethanol concentration of 61%, and a liquid-to-material ratio of 39:1, resulting in an AHP-EWM composite score of 94.76. Comparative analysis of antibacterial and antioxidant activities revealed that ASC extracts exhibited superior antibacterial effects, while ASF extracts demonstrated enhanced antioxidant properties. These findings support the partial replacement of ASC with ASF, contributing to the conservation of wild resources and expanding the application of ASF in medicinal development. |
| format | Article |
| id | doaj-art-41b25e46f8ff410da217139cb4a54c7c |
| institution | Kabale University |
| issn | 1420-3049 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj-art-41b25e46f8ff410da217139cb4a54c7c2025-01-24T13:43:54ZengMDPI AGMolecules1420-30492025-01-0130239710.3390/molecules30020397Ultrasound-Assisted Enzymatic Extraction of the Active Components from <i>Acanthopanax sessiliflorus</i> Stem and Bioactivity Comparison with <i>Acanthopanax senticosus</i>Qiaomu You0Yanjun Ren1Jiaotong Li2Guangnian Zeng3Xiongfei Luo4Chunying Zheng5Zhonghua Tang6Key Laboratory of Forest Plant Ecology of Ministry of Education, Northeast Forestry University, Hexing Road 26, Harbin 150040, ChinaKey Laboratory of Forest Plant Ecology of Ministry of Education, Northeast Forestry University, Hexing Road 26, Harbin 150040, ChinaKey Laboratory of Forest Plant Ecology of Ministry of Education, Northeast Forestry University, Hexing Road 26, Harbin 150040, ChinaKey Laboratory of Forest Plant Ecology of Ministry of Education, Northeast Forestry University, Hexing Road 26, Harbin 150040, ChinaKey Laboratory of Forest Plant Ecology of Ministry of Education, Northeast Forestry University, Hexing Road 26, Harbin 150040, ChinaEngineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region & Key Laboratory of Microbiology, College of Heilongiang Province & School of Life Sciences, Heilongiiang University, Harbin 150080, ChinaKey Laboratory of Forest Plant Ecology of Ministry of Education, Northeast Forestry University, Hexing Road 26, Harbin 150040, China<i>Acanthopanax senticosus</i> (ASC) contains a variety of bioactive compounds and serves as an important traditional Chinese medicinal resource. However, its prolonged growth cycle and reliance on wild populations limit its practical use. To explore the potential of <i>Acanthopanax sessiliflorus</i> (ASF) as an alternative, this study focused on optimizing the extraction process and assessing the bioactivity of stem extracts. The Analytic Hierarchy Process-Entropy Weight Method (AHP-EWM) was applied to comprehensively score five key active compounds in ASF stems, establishing a benchmark for evaluating extraction efficiency. Ultrasound-assisted enzymatic extraction (UAEE) was employed, and orthogonal and response surface experiments were conducted to refine the extraction parameters. The optimal conditions identified were an ultrasound temperature of 59 °C, a duration of 57 min, an ethanol concentration of 61%, and a liquid-to-material ratio of 39:1, resulting in an AHP-EWM composite score of 94.76. Comparative analysis of antibacterial and antioxidant activities revealed that ASC extracts exhibited superior antibacterial effects, while ASF extracts demonstrated enhanced antioxidant properties. These findings support the partial replacement of ASC with ASF, contributing to the conservation of wild resources and expanding the application of ASF in medicinal development.https://www.mdpi.com/1420-3049/30/2/397<i>Acanthopanax sessiliflorus</i>ultrasound-assisted enzymatic extractionantioxidantantibacterialutilization of plant resources |
| spellingShingle | Qiaomu You Yanjun Ren Jiaotong Li Guangnian Zeng Xiongfei Luo Chunying Zheng Zhonghua Tang Ultrasound-Assisted Enzymatic Extraction of the Active Components from <i>Acanthopanax sessiliflorus</i> Stem and Bioactivity Comparison with <i>Acanthopanax senticosus</i> Molecules <i>Acanthopanax sessiliflorus</i> ultrasound-assisted enzymatic extraction antioxidant antibacterial utilization of plant resources |
| title | Ultrasound-Assisted Enzymatic Extraction of the Active Components from <i>Acanthopanax sessiliflorus</i> Stem and Bioactivity Comparison with <i>Acanthopanax senticosus</i> |
| title_full | Ultrasound-Assisted Enzymatic Extraction of the Active Components from <i>Acanthopanax sessiliflorus</i> Stem and Bioactivity Comparison with <i>Acanthopanax senticosus</i> |
| title_fullStr | Ultrasound-Assisted Enzymatic Extraction of the Active Components from <i>Acanthopanax sessiliflorus</i> Stem and Bioactivity Comparison with <i>Acanthopanax senticosus</i> |
| title_full_unstemmed | Ultrasound-Assisted Enzymatic Extraction of the Active Components from <i>Acanthopanax sessiliflorus</i> Stem and Bioactivity Comparison with <i>Acanthopanax senticosus</i> |
| title_short | Ultrasound-Assisted Enzymatic Extraction of the Active Components from <i>Acanthopanax sessiliflorus</i> Stem and Bioactivity Comparison with <i>Acanthopanax senticosus</i> |
| title_sort | ultrasound assisted enzymatic extraction of the active components from i acanthopanax sessiliflorus i stem and bioactivity comparison with i acanthopanax senticosus i |
| topic | <i>Acanthopanax sessiliflorus</i> ultrasound-assisted enzymatic extraction antioxidant antibacterial utilization of plant resources |
| url | https://www.mdpi.com/1420-3049/30/2/397 |
| work_keys_str_mv | AT qiaomuyou ultrasoundassistedenzymaticextractionoftheactivecomponentsfromiacanthopanaxsessiliflorusistemandbioactivitycomparisonwithiacanthopanaxsenticosusi AT yanjunren ultrasoundassistedenzymaticextractionoftheactivecomponentsfromiacanthopanaxsessiliflorusistemandbioactivitycomparisonwithiacanthopanaxsenticosusi AT jiaotongli ultrasoundassistedenzymaticextractionoftheactivecomponentsfromiacanthopanaxsessiliflorusistemandbioactivitycomparisonwithiacanthopanaxsenticosusi AT guangnianzeng ultrasoundassistedenzymaticextractionoftheactivecomponentsfromiacanthopanaxsessiliflorusistemandbioactivitycomparisonwithiacanthopanaxsenticosusi AT xiongfeiluo ultrasoundassistedenzymaticextractionoftheactivecomponentsfromiacanthopanaxsessiliflorusistemandbioactivitycomparisonwithiacanthopanaxsenticosusi AT chunyingzheng ultrasoundassistedenzymaticextractionoftheactivecomponentsfromiacanthopanaxsessiliflorusistemandbioactivitycomparisonwithiacanthopanaxsenticosusi AT zhonghuatang ultrasoundassistedenzymaticextractionoftheactivecomponentsfromiacanthopanaxsessiliflorusistemandbioactivitycomparisonwithiacanthopanaxsenticosusi |