COMPARATIVE RETROTRANSPOSON ANALYSIS in WHEAT
The presence of retrotransposons is associated with polyploidy, especially in wheat, and may cause an increase in genome size. In this study, the evolutionary information was aimed to reveal based on the comparison retrotrans-poson movements between bread and einkorn wheat Siyez. For that reason, th...
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Çanakkale Onsekiz Mart University
2021-09-01
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| Series: | Journal of Advanced Research in Natural and Applied Sciences |
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| Online Access: | https://dergipark.org.tr/en/download/article-file/1857707 |
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| author | Elif Karlık Seray Altıntaş Bekir Ahmet Ilgar |
| author_facet | Elif Karlık Seray Altıntaş Bekir Ahmet Ilgar |
| author_sort | Elif Karlık |
| collection | DOAJ |
| description | The presence of retrotransposons is associated with polyploidy, especially in wheat, and may cause an increase in genome size. In this study, the evolutionary information was aimed to reveal based on the comparison retrotrans-poson movements between bread and einkorn wheat Siyez. For that reason, the transposition of BARE1, Sukkula and Nikita retrotransposons in bread and einkorn wheat Siyez were analysed by using IRAP-PCR molecular mark-er method. Both monomorphic and polymorphic bands in each wheat species have been demonstrated. IRAP-PCR products of Sukkula retrotransposon was showed as 10 bands in bread wheat, but no bands could be deter-mined in einkorn wheat. Nikita retrotransposon was demonstrated as 6 bands in bread wheat, 14 bands in einkorn wheat Siyez. Polymorphism rate was calculated as 81% for Nikita between bread wheat and einkorn wheat Siyez. However, the presence of BARE1 were not observed in both species. The obtained findings suggest that Nikita retrotransposon contributes to genome obesity, especially in bread wheat. The failure of Sukkula retrotransposon detection in einkorn wheat Siyez indicates that Sukkula may be inserted in the genome of bread wheat by horizon-tal gene transfer during wheat domestication events. These results may provide to uncover the organization of wheat genome during domestication. |
| format | Article |
| id | doaj-art-4167dc1528b1406dbbd0b4003837e4cf |
| institution | Kabale University |
| issn | 2757-5195 |
| language | English |
| publishDate | 2021-09-01 |
| publisher | Çanakkale Onsekiz Mart University |
| record_format | Article |
| series | Journal of Advanced Research in Natural and Applied Sciences |
| spelling | doaj-art-4167dc1528b1406dbbd0b4003837e4cf2025-02-05T17:58:10ZengÇanakkale Onsekiz Mart UniversityJournal of Advanced Research in Natural and Applied Sciences2757-51952021-09-017336937410.28979/jarnas.961832453COMPARATIVE RETROTRANSPOSON ANALYSIS in WHEATElif Karlık0https://orcid.org/0000-0003-0669-2725Seray Altıntaş1https://orcid.org/0000-0001-9215-8435Bekir Ahmet Ilgar2https://orcid.org/0000-0001-7907-8465ISTINYE UNIVERSITYISTINYE UNIVERSITYISTINYE UNIVERSITYThe presence of retrotransposons is associated with polyploidy, especially in wheat, and may cause an increase in genome size. In this study, the evolutionary information was aimed to reveal based on the comparison retrotrans-poson movements between bread and einkorn wheat Siyez. For that reason, the transposition of BARE1, Sukkula and Nikita retrotransposons in bread and einkorn wheat Siyez were analysed by using IRAP-PCR molecular mark-er method. Both monomorphic and polymorphic bands in each wheat species have been demonstrated. IRAP-PCR products of Sukkula retrotransposon was showed as 10 bands in bread wheat, but no bands could be deter-mined in einkorn wheat. Nikita retrotransposon was demonstrated as 6 bands in bread wheat, 14 bands in einkorn wheat Siyez. Polymorphism rate was calculated as 81% for Nikita between bread wheat and einkorn wheat Siyez. However, the presence of BARE1 were not observed in both species. The obtained findings suggest that Nikita retrotransposon contributes to genome obesity, especially in bread wheat. The failure of Sukkula retrotransposon detection in einkorn wheat Siyez indicates that Sukkula may be inserted in the genome of bread wheat by horizon-tal gene transfer during wheat domestication events. These results may provide to uncover the organization of wheat genome during domestication.https://dergipark.org.tr/en/download/article-file/1857707einkorn wheat siyezbread wheatnikitasukkulabare1 |
| spellingShingle | Elif Karlık Seray Altıntaş Bekir Ahmet Ilgar COMPARATIVE RETROTRANSPOSON ANALYSIS in WHEAT Journal of Advanced Research in Natural and Applied Sciences einkorn wheat siyez bread wheat nikita sukkula bare1 |
| title | COMPARATIVE RETROTRANSPOSON ANALYSIS in WHEAT |
| title_full | COMPARATIVE RETROTRANSPOSON ANALYSIS in WHEAT |
| title_fullStr | COMPARATIVE RETROTRANSPOSON ANALYSIS in WHEAT |
| title_full_unstemmed | COMPARATIVE RETROTRANSPOSON ANALYSIS in WHEAT |
| title_short | COMPARATIVE RETROTRANSPOSON ANALYSIS in WHEAT |
| title_sort | comparative retrotransposon analysis in wheat |
| topic | einkorn wheat siyez bread wheat nikita sukkula bare1 |
| url | https://dergipark.org.tr/en/download/article-file/1857707 |
| work_keys_str_mv | AT elifkarlık comparativeretrotransposonanalysisinwheat AT serayaltıntas comparativeretrotransposonanalysisinwheat AT bekirahmetilgar comparativeretrotransposonanalysisinwheat |