Emergence of Carbapenem-Resistant <i>bla</i><sub>POM-1</sub> Harboring <i>Pseudomonas otitidis</i> Isolated from River Water in Ghana
<b>Introduction:</b> <i>Pseudomonas otitidis</i>, known for carrying the <i>bla</i><sub>POM-1</sub> gene and linked to various diseases, is widely distributed. However, its prevalence in Ghana is unknown, mainly due to misidentification or inadequate r...
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2025-01-01
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| author | Frederick Ofosu Appiah Samiratu Mahazu Isaac Prah Taira Kawamura Yusuke Ota Yohei Nishikawa Mitsunori Yoshida Masato Suzuki Yoshihiko Hoshino Toshihiko Suzuki Tomoko Ishino Anthony Ablordey Ryoichi Saito |
| author_facet | Frederick Ofosu Appiah Samiratu Mahazu Isaac Prah Taira Kawamura Yusuke Ota Yohei Nishikawa Mitsunori Yoshida Masato Suzuki Yoshihiko Hoshino Toshihiko Suzuki Tomoko Ishino Anthony Ablordey Ryoichi Saito |
| author_sort | Frederick Ofosu Appiah |
| collection | DOAJ |
| description | <b>Introduction:</b> <i>Pseudomonas otitidis</i>, known for carrying the <i>bla</i><sub>POM-1</sub> gene and linked to various diseases, is widely distributed. However, its prevalence in Ghana is unknown, mainly due to misidentification or inadequate research. In this study, for the first time, we characterized <i>P. otitidis</i> from Densu river water in Ghana. <b>Methods:</b> The antimicrobial susceptibility and whole genome characteristics of two strains (Tg_9B and BC12) were determined. The resistance and virulence features were determined using ResFinder and the VFDB database, respectively. Maximum-likelihood phylogeny was conducted based on amino acid sequences of <i>bla</i><sub>POM-1</sub> and <i>P. otitidis</i> core genomes. <b>Results:</b> The strains carried <i>bla</i><sub>POM-1</sub> on the chromosome, with only Tg_9B showing intermediate resistance to meropenem. Tg_9B had a unique genetic make-up downstream of <i>bla</i><sub>POM-1</sub>, compared with BC12 and other reference strains. Both strains harbored virulence factors able to induce pathogenicity through immune evasion. The efflux pump genes (<i>adeF</i>, <i>rsmA</i>, and <i>qacG</i>) were present in the genomes of all the strains used in this study. The amino acid sequences of <i>POM-1</i> in the strains shared a sequence homology with seven other sequences from different countries. <b>Conclusions:</b> This study highlights the emergence of <i>bla</i><sub>POM-1</sub> harboring <i>P. otitidis</i> in Ghana and affirms the conservation of <i>bla</i><sub>POM-1</sub> and <i>adeF</i>, <i>rsmA</i>, and <i>qacG</i> in the species. |
| format | Article |
| id | doaj-art-4128f03649d14f109457876c5a24f9f6 |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-4128f03649d14f109457876c5a24f9f62025-01-24T13:18:44ZengMDPI AGAntibiotics2079-63822025-01-011415010.3390/antibiotics14010050Emergence of Carbapenem-Resistant <i>bla</i><sub>POM-1</sub> Harboring <i>Pseudomonas otitidis</i> Isolated from River Water in GhanaFrederick Ofosu Appiah0Samiratu Mahazu1Isaac Prah2Taira Kawamura3Yusuke Ota4Yohei Nishikawa5Mitsunori Yoshida6Masato Suzuki7Yoshihiko Hoshino8Toshihiko Suzuki9Tomoko Ishino10Anthony Ablordey11Ryoichi Saito12Department of Molecular Microbiology and Immunology, Institute of Science Tokyo, Tokyo 113-8510, JapanDepartment of Molecular Microbiology and Immunology, Institute of Science Tokyo, Tokyo 113-8510, JapanDepartment of Molecular Microbiology and Immunology, Institute of Science Tokyo, Tokyo 113-8510, JapanDepartment of Molecular Microbiology and Immunology, Institute of Science Tokyo, Tokyo 113-8510, JapanDepartment of Molecular Microbiology and Immunology, Institute of Science Tokyo, Tokyo 113-8510, JapanComputational Bio Big-Data Open Innovation Laboratory, AIST-Waseda University, Tokyo 169-0082, JapanDepartment of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases, Tokyo 162-8640, JapanAntimicrobial Resistance Research Center, National Institute of Infectious Diseases, Tokyo 162-8640, JapanDepartment of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases, Tokyo 162-8640, JapanDepartment of Bacterial Pathogenesis, Tokyo Medical and Dental University, Tokyo 113-8510, JapanDepartment of Parasitology and Tropical Medicine, Institute of Science Tokyo, Tokyo 113-8510, JapanDepartment of Bacteriology, Noguchi Memorial Institute for Medical Research, Accra P.O. Box LG43, GhanaDepartment of Molecular Microbiology and Immunology, Institute of Science Tokyo, Tokyo 113-8510, Japan<b>Introduction:</b> <i>Pseudomonas otitidis</i>, known for carrying the <i>bla</i><sub>POM-1</sub> gene and linked to various diseases, is widely distributed. However, its prevalence in Ghana is unknown, mainly due to misidentification or inadequate research. In this study, for the first time, we characterized <i>P. otitidis</i> from Densu river water in Ghana. <b>Methods:</b> The antimicrobial susceptibility and whole genome characteristics of two strains (Tg_9B and BC12) were determined. The resistance and virulence features were determined using ResFinder and the VFDB database, respectively. Maximum-likelihood phylogeny was conducted based on amino acid sequences of <i>bla</i><sub>POM-1</sub> and <i>P. otitidis</i> core genomes. <b>Results:</b> The strains carried <i>bla</i><sub>POM-1</sub> on the chromosome, with only Tg_9B showing intermediate resistance to meropenem. Tg_9B had a unique genetic make-up downstream of <i>bla</i><sub>POM-1</sub>, compared with BC12 and other reference strains. Both strains harbored virulence factors able to induce pathogenicity through immune evasion. The efflux pump genes (<i>adeF</i>, <i>rsmA</i>, and <i>qacG</i>) were present in the genomes of all the strains used in this study. The amino acid sequences of <i>POM-1</i> in the strains shared a sequence homology with seven other sequences from different countries. <b>Conclusions:</b> This study highlights the emergence of <i>bla</i><sub>POM-1</sub> harboring <i>P. otitidis</i> in Ghana and affirms the conservation of <i>bla</i><sub>POM-1</sub> and <i>adeF</i>, <i>rsmA</i>, and <i>qacG</i> in the species.https://www.mdpi.com/2079-6382/14/1/50<i>Pseudomonas otitidis</i><i>Pseudomonas aeruginosa</i>metallo-β-lactamases POM-1carbapenemsvirulence factorsmaximum-likelihood phylogeny |
| spellingShingle | Frederick Ofosu Appiah Samiratu Mahazu Isaac Prah Taira Kawamura Yusuke Ota Yohei Nishikawa Mitsunori Yoshida Masato Suzuki Yoshihiko Hoshino Toshihiko Suzuki Tomoko Ishino Anthony Ablordey Ryoichi Saito Emergence of Carbapenem-Resistant <i>bla</i><sub>POM-1</sub> Harboring <i>Pseudomonas otitidis</i> Isolated from River Water in Ghana Antibiotics <i>Pseudomonas otitidis</i> <i>Pseudomonas aeruginosa</i> metallo-β-lactamases POM-1 carbapenems virulence factors maximum-likelihood phylogeny |
| title | Emergence of Carbapenem-Resistant <i>bla</i><sub>POM-1</sub> Harboring <i>Pseudomonas otitidis</i> Isolated from River Water in Ghana |
| title_full | Emergence of Carbapenem-Resistant <i>bla</i><sub>POM-1</sub> Harboring <i>Pseudomonas otitidis</i> Isolated from River Water in Ghana |
| title_fullStr | Emergence of Carbapenem-Resistant <i>bla</i><sub>POM-1</sub> Harboring <i>Pseudomonas otitidis</i> Isolated from River Water in Ghana |
| title_full_unstemmed | Emergence of Carbapenem-Resistant <i>bla</i><sub>POM-1</sub> Harboring <i>Pseudomonas otitidis</i> Isolated from River Water in Ghana |
| title_short | Emergence of Carbapenem-Resistant <i>bla</i><sub>POM-1</sub> Harboring <i>Pseudomonas otitidis</i> Isolated from River Water in Ghana |
| title_sort | emergence of carbapenem resistant i bla i sub pom 1 sub harboring i pseudomonas otitidis i isolated from river water in ghana |
| topic | <i>Pseudomonas otitidis</i> <i>Pseudomonas aeruginosa</i> metallo-β-lactamases POM-1 carbapenems virulence factors maximum-likelihood phylogeny |
| url | https://www.mdpi.com/2079-6382/14/1/50 |
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