Single-atom Zr doped heterojunction enhanced piezocatalysis for implant infection therapy through synergistic metal immunotherapy with sonodynamic and physical puncture

Abstract Recent common clinical treatments for implant bacterial infections involve replacing inert implants and using antibiotics. However, these methods remain limited in their effectiveness for pathogen clearance, immune regulation, and osteogenesis. In this study, we developed a Zr-doped heteroi...

Full description

Saved in:
Bibliographic Details
Main Authors: Xing Zhou, Jingbo Xie, Xingchen Zhou, Tianyou Ma, Yichen Lu, Yiwen Yang, Zhefei Xie, Houfu Ling, Rui Xu, Mo Wu, Jinglei Wang, Weixiang Wang, Derong Kong, Pengchao Xu, Xuan Wan, Hongbo Wu, Peijian Tong, Hanting Xia
Format: Article
Language:English
Published: BMC 2025-03-01
Series:Journal of Nanobiotechnology
Subjects:
Online Access:https://doi.org/10.1186/s12951-025-03309-x
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849392393584902144
author Xing Zhou
Jingbo Xie
Xingchen Zhou
Tianyou Ma
Yichen Lu
Yiwen Yang
Zhefei Xie
Houfu Ling
Rui Xu
Mo Wu
Jinglei Wang
Weixiang Wang
Derong Kong
Pengchao Xu
Xuan Wan
Hongbo Wu
Peijian Tong
Hanting Xia
author_facet Xing Zhou
Jingbo Xie
Xingchen Zhou
Tianyou Ma
Yichen Lu
Yiwen Yang
Zhefei Xie
Houfu Ling
Rui Xu
Mo Wu
Jinglei Wang
Weixiang Wang
Derong Kong
Pengchao Xu
Xuan Wan
Hongbo Wu
Peijian Tong
Hanting Xia
author_sort Xing Zhou
collection DOAJ
description Abstract Recent common clinical treatments for implant bacterial infections involve replacing inert implants and using antibiotics. However, these methods remain limited in their effectiveness for pathogen clearance, immune regulation, and osteogenesis. In this study, we developed a Zr-doped heterointerface of SrTiO3 and Hap (SrTiZrO3/Hap) heterojunction coating with single-atom Zr doping and heterogeneous interfaces designed for ultrasound-responsive antimicrobial activity and bone formation. Under ultrasound, the mechanical force exerted by SrTiZrO3/Hap enhances its physical puncture and sonodynamic activity, synergizing with the metalloimmunotherapy effect of Zr4+ for efficient antimicrobial activity. The primary mechanism enhancing sonodynamic activity involves local interfacial polarization from single-atom Zr doping, achieving piezoelectric catalysis in conjunction with electronic polarization from the built-in electric field. SrTiZrO3/Hap achieved a 99.3% antibacterial rate against S. aureus and 99.7% against E. coli under ultrasound. Additionally, SrTiZrO3/Hap promoted osteogenic differentiation after ultrasound irradiation by activating the PI3K/Akt pathway via its piezoelectric, needle-like topological surface and the release of functional ions, thus accelerating bone repair.
format Article
id doaj-art-407f950d54b1423ab1e8f05eb201bbfe
institution Kabale University
issn 1477-3155
language English
publishDate 2025-03-01
publisher BMC
record_format Article
series Journal of Nanobiotechnology
spelling doaj-art-407f950d54b1423ab1e8f05eb201bbfe2025-08-20T03:40:47ZengBMCJournal of Nanobiotechnology1477-31552025-03-0123111910.1186/s12951-025-03309-xSingle-atom Zr doped heterojunction enhanced piezocatalysis for implant infection therapy through synergistic metal immunotherapy with sonodynamic and physical punctureXing Zhou0Jingbo Xie1Xingchen Zhou2Tianyou Ma3Yichen Lu4Yiwen Yang5Zhefei Xie6Houfu Ling7Rui Xu8Mo Wu9Jinglei Wang10Weixiang Wang11Derong Kong12Pengchao Xu13Xuan Wan14Hongbo Wu15Peijian Tong16Hanting Xia17The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), Zhejiang Chinese Medical UniversityDepartment of Orthopaedics, The People’s Hospital of Fengcheng CityThe First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), Zhejiang Chinese Medical UniversityThe First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), Zhejiang Chinese Medical UniversityThe First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), Zhejiang Chinese Medical UniversityThe First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), Zhejiang Chinese Medical UniversityThe First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), Zhejiang Chinese Medical UniversityThe First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), Zhejiang Chinese Medical UniversityDepartment of Orthopaedics, Affiliated Hospital of Jiangxi University of Chinese Medicine, Jiangxi University of Chinese MedicineThe First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), Zhejiang Chinese Medical UniversityThe First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), Zhejiang Chinese Medical UniversityThe First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), Zhejiang Chinese Medical UniversityState Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan UniversityThe First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), Zhejiang Chinese Medical UniversityDepartment of Orthopaedics, Affiliated Hospital of Jiangxi University of Chinese Medicine, Jiangxi University of Chinese MedicineDepartment of Rehabilitation Medicine, Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, The Fifth Affiliated Hospital, Guangzhou Medical UniversityThe First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), Zhejiang Chinese Medical UniversityThe First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), Zhejiang Chinese Medical UniversityAbstract Recent common clinical treatments for implant bacterial infections involve replacing inert implants and using antibiotics. However, these methods remain limited in their effectiveness for pathogen clearance, immune regulation, and osteogenesis. In this study, we developed a Zr-doped heterointerface of SrTiO3 and Hap (SrTiZrO3/Hap) heterojunction coating with single-atom Zr doping and heterogeneous interfaces designed for ultrasound-responsive antimicrobial activity and bone formation. Under ultrasound, the mechanical force exerted by SrTiZrO3/Hap enhances its physical puncture and sonodynamic activity, synergizing with the metalloimmunotherapy effect of Zr4+ for efficient antimicrobial activity. The primary mechanism enhancing sonodynamic activity involves local interfacial polarization from single-atom Zr doping, achieving piezoelectric catalysis in conjunction with electronic polarization from the built-in electric field. SrTiZrO3/Hap achieved a 99.3% antibacterial rate against S. aureus and 99.7% against E. coli under ultrasound. Additionally, SrTiZrO3/Hap promoted osteogenic differentiation after ultrasound irradiation by activating the PI3K/Akt pathway via its piezoelectric, needle-like topological surface and the release of functional ions, thus accelerating bone repair.https://doi.org/10.1186/s12951-025-03309-xPiezocatalysisSonodynamic therapyMetalloimmunotherapyAntibacterialBone formation
spellingShingle Xing Zhou
Jingbo Xie
Xingchen Zhou
Tianyou Ma
Yichen Lu
Yiwen Yang
Zhefei Xie
Houfu Ling
Rui Xu
Mo Wu
Jinglei Wang
Weixiang Wang
Derong Kong
Pengchao Xu
Xuan Wan
Hongbo Wu
Peijian Tong
Hanting Xia
Single-atom Zr doped heterojunction enhanced piezocatalysis for implant infection therapy through synergistic metal immunotherapy with sonodynamic and physical puncture
Journal of Nanobiotechnology
Piezocatalysis
Sonodynamic therapy
Metalloimmunotherapy
Antibacterial
Bone formation
title Single-atom Zr doped heterojunction enhanced piezocatalysis for implant infection therapy through synergistic metal immunotherapy with sonodynamic and physical puncture
title_full Single-atom Zr doped heterojunction enhanced piezocatalysis for implant infection therapy through synergistic metal immunotherapy with sonodynamic and physical puncture
title_fullStr Single-atom Zr doped heterojunction enhanced piezocatalysis for implant infection therapy through synergistic metal immunotherapy with sonodynamic and physical puncture
title_full_unstemmed Single-atom Zr doped heterojunction enhanced piezocatalysis for implant infection therapy through synergistic metal immunotherapy with sonodynamic and physical puncture
title_short Single-atom Zr doped heterojunction enhanced piezocatalysis for implant infection therapy through synergistic metal immunotherapy with sonodynamic and physical puncture
title_sort single atom zr doped heterojunction enhanced piezocatalysis for implant infection therapy through synergistic metal immunotherapy with sonodynamic and physical puncture
topic Piezocatalysis
Sonodynamic therapy
Metalloimmunotherapy
Antibacterial
Bone formation
url https://doi.org/10.1186/s12951-025-03309-x
work_keys_str_mv AT xingzhou singleatomzrdopedheterojunctionenhancedpiezocatalysisforimplantinfectiontherapythroughsynergisticmetalimmunotherapywithsonodynamicandphysicalpuncture
AT jingboxie singleatomzrdopedheterojunctionenhancedpiezocatalysisforimplantinfectiontherapythroughsynergisticmetalimmunotherapywithsonodynamicandphysicalpuncture
AT xingchenzhou singleatomzrdopedheterojunctionenhancedpiezocatalysisforimplantinfectiontherapythroughsynergisticmetalimmunotherapywithsonodynamicandphysicalpuncture
AT tianyouma singleatomzrdopedheterojunctionenhancedpiezocatalysisforimplantinfectiontherapythroughsynergisticmetalimmunotherapywithsonodynamicandphysicalpuncture
AT yichenlu singleatomzrdopedheterojunctionenhancedpiezocatalysisforimplantinfectiontherapythroughsynergisticmetalimmunotherapywithsonodynamicandphysicalpuncture
AT yiwenyang singleatomzrdopedheterojunctionenhancedpiezocatalysisforimplantinfectiontherapythroughsynergisticmetalimmunotherapywithsonodynamicandphysicalpuncture
AT zhefeixie singleatomzrdopedheterojunctionenhancedpiezocatalysisforimplantinfectiontherapythroughsynergisticmetalimmunotherapywithsonodynamicandphysicalpuncture
AT houfuling singleatomzrdopedheterojunctionenhancedpiezocatalysisforimplantinfectiontherapythroughsynergisticmetalimmunotherapywithsonodynamicandphysicalpuncture
AT ruixu singleatomzrdopedheterojunctionenhancedpiezocatalysisforimplantinfectiontherapythroughsynergisticmetalimmunotherapywithsonodynamicandphysicalpuncture
AT mowu singleatomzrdopedheterojunctionenhancedpiezocatalysisforimplantinfectiontherapythroughsynergisticmetalimmunotherapywithsonodynamicandphysicalpuncture
AT jingleiwang singleatomzrdopedheterojunctionenhancedpiezocatalysisforimplantinfectiontherapythroughsynergisticmetalimmunotherapywithsonodynamicandphysicalpuncture
AT weixiangwang singleatomzrdopedheterojunctionenhancedpiezocatalysisforimplantinfectiontherapythroughsynergisticmetalimmunotherapywithsonodynamicandphysicalpuncture
AT derongkong singleatomzrdopedheterojunctionenhancedpiezocatalysisforimplantinfectiontherapythroughsynergisticmetalimmunotherapywithsonodynamicandphysicalpuncture
AT pengchaoxu singleatomzrdopedheterojunctionenhancedpiezocatalysisforimplantinfectiontherapythroughsynergisticmetalimmunotherapywithsonodynamicandphysicalpuncture
AT xuanwan singleatomzrdopedheterojunctionenhancedpiezocatalysisforimplantinfectiontherapythroughsynergisticmetalimmunotherapywithsonodynamicandphysicalpuncture
AT hongbowu singleatomzrdopedheterojunctionenhancedpiezocatalysisforimplantinfectiontherapythroughsynergisticmetalimmunotherapywithsonodynamicandphysicalpuncture
AT peijiantong singleatomzrdopedheterojunctionenhancedpiezocatalysisforimplantinfectiontherapythroughsynergisticmetalimmunotherapywithsonodynamicandphysicalpuncture
AT hantingxia singleatomzrdopedheterojunctionenhancedpiezocatalysisforimplantinfectiontherapythroughsynergisticmetalimmunotherapywithsonodynamicandphysicalpuncture