Phenotyping determination of cyp1a2 enzyme activity using caffeine in sheep
<b>Aim:</b> The aims of this study were to determine the validity of the plasma metabolic ratios (MR) to investigate the CYP1A2 activity in practice and to compare in vivo CYP1A2 enzyme activity using caffeine (CF) as a probe in Morkaraman (MK), Akkaraman (AK) and Anatolia Merino (AM) sh...
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| Format: | Article |
| Language: | English |
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Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
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| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=543 |
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| author | Kamil Uney Bünyamin Tras |
| author_facet | Kamil Uney Bünyamin Tras |
| author_sort | Kamil Uney |
| collection | DOAJ |
| description | <b>Aim:</b> The aims of this study were to determine the validity of
the plasma metabolic ratios (MR) to investigate the CYP1A2
activity in practice and to compare in vivo CYP1A2 enzyme
activity using caffeine (CF) as a probe in Morkaraman (MK),
Akkaraman (AK) and Anatolia Merino (AM) sheep breeds.<p>
<b>Materials and Methods:</b> Caffeine was administered as a
single dose of 5 mg/kg b.w. by the intravenous in MK, AK
and AM sheep breeds. The plasma levels of CF and paraxanthine
(PX) were measured using high-performance liquid
chromatography. CYP1A2 phenotyping was measured using
the ratio [(PX/CF)AUC] between areas under the plasma
concentration-time curve (AUCs) of PX and CF and the ratios
[(PX/CF)MR→3-16 h] between plasma concentrations of
PX and CF at 3 to 16 h after CF administration. Correlations
between the plasma (PX/CF)MR→3-16 h and (PX/CF)AUC ratios
were determined to investigate of the CYP1A2 phenotyping
by single blood sampling in practice.<p>
<b>Results:</b> It was determined that the more reliable sampling
time within the plasma (PX/CF)MR→3-16 h ratios in the determination
of the CYP1A2 phenotyping was 10 h after CF
administration. (PX/CF)MR→10 h and (PX/CF)AUC ratios were
similar (p>0.05) among sheep breeds.<p>
<b>Conclusion:</b> The plasma (PX/CF)MR→10 h ratio might be used
as a rapid and simple screening test for CYP1A2 phenotyping
in sheep. CYP1A2 enzyme may not be clinically important
in the observed differences to the effects of drugs and
environmental chemicals with its substrates among MK, AK
and AM sheep breeds. |
| format | Article |
| id | doaj-art-401ad5d71efb48e0b6fcca547ab1d355 |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-401ad5d71efb48e0b6fcca547ab1d3552025-02-03T11:25:41ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19532712732543Phenotyping determination of cyp1a2 enzyme activity using caffeine in sheepKamil UneyBünyamin Tras<b>Aim:</b> The aims of this study were to determine the validity of the plasma metabolic ratios (MR) to investigate the CYP1A2 activity in practice and to compare in vivo CYP1A2 enzyme activity using caffeine (CF) as a probe in Morkaraman (MK), Akkaraman (AK) and Anatolia Merino (AM) sheep breeds.<p> <b>Materials and Methods:</b> Caffeine was administered as a single dose of 5 mg/kg b.w. by the intravenous in MK, AK and AM sheep breeds. The plasma levels of CF and paraxanthine (PX) were measured using high-performance liquid chromatography. CYP1A2 phenotyping was measured using the ratio [(PX/CF)AUC] between areas under the plasma concentration-time curve (AUCs) of PX and CF and the ratios [(PX/CF)MR→3-16 h] between plasma concentrations of PX and CF at 3 to 16 h after CF administration. Correlations between the plasma (PX/CF)MR→3-16 h and (PX/CF)AUC ratios were determined to investigate of the CYP1A2 phenotyping by single blood sampling in practice.<p> <b>Results:</b> It was determined that the more reliable sampling time within the plasma (PX/CF)MR→3-16 h ratios in the determination of the CYP1A2 phenotyping was 10 h after CF administration. (PX/CF)MR→10 h and (PX/CF)AUC ratios were similar (p>0.05) among sheep breeds.<p> <b>Conclusion:</b> The plasma (PX/CF)MR→10 h ratio might be used as a rapid and simple screening test for CYP1A2 phenotyping in sheep. CYP1A2 enzyme may not be clinically important in the observed differences to the effects of drugs and environmental chemicals with its substrates among MK, AK and AM sheep breeds.http://eurasianjvetsci.org/pdf.php3?id=543caffeinecyp1a2 activitysheepbreed |
| spellingShingle | Kamil Uney Bünyamin Tras Phenotyping determination of cyp1a2 enzyme activity using caffeine in sheep Eurasian Journal of Veterinary Sciences caffeine cyp1a2 activity sheep breed |
| title | Phenotyping determination of cyp1a2 enzyme activity using caffeine in sheep |
| title_full | Phenotyping determination of cyp1a2 enzyme activity using caffeine in sheep |
| title_fullStr | Phenotyping determination of cyp1a2 enzyme activity using caffeine in sheep |
| title_full_unstemmed | Phenotyping determination of cyp1a2 enzyme activity using caffeine in sheep |
| title_short | Phenotyping determination of cyp1a2 enzyme activity using caffeine in sheep |
| title_sort | phenotyping determination of cyp1a2 enzyme activity using caffeine in sheep |
| topic | caffeine cyp1a2 activity sheep breed |
| url | http://eurasianjvetsci.org/pdf.php3?id=543 |
| work_keys_str_mv | AT kamiluney phenotypingdeterminationofcyp1a2enzymeactivityusingcaffeineinsheep AT bunyamintras phenotypingdeterminationofcyp1a2enzymeactivityusingcaffeineinsheep |