白蛋白对近端肾小管上皮细胞增殖与凋亡的影响

【目的】探讨白蛋白对近端肾小管上皮细胞(PTCs)增殖与凋亡的影响。【方法】用不同剂量(0.1,0.5,1,5,10,30mg/mL)去脂牛血清白蛋白(dBSA)超载体外培养的NRK52E,dBSA 0mg/mL作为对照。细胞增殖活性用MTS比色法检测和显微镜观察。细胞凋亡检测:荧光染料DAPI染核,DNA梯带实验,原位末端标记(TUNEL)实验和苔盼蓝染色计数。【结果】高剂量dBSA超载未完全融合NRK52E细胞144h后细胞变得更密集。MTS比色结果发现,高剂量dBSA(5、10、30mg/mL)超载可明显诱导NRK52E细胞增殖,增殖活性分别为(0.700±0.045)A、(1.021±...

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Bibliographic Details
Main Authors: 孙良忠, 岳智慧, 陈述枚
Format: Article
Language:zho
Published: Editorial Office of Journal of Sun Yat-sen University 2007-01-01
Series:Zhongshan Daxue xuebao. Yixue kexue ban
Subjects:
Online Access:http://xuebaoyx.sysu.edu.cn/zh/article/43581129/
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