Construction and Investigation of MicroRNA-mRNA Regulatory Network of Gastric Cancer with Helicobacter pylori Infection
Background. Helicobacter pylori (H. pylori) is a common human pathogen, which is closely correlated with gastric cancer (GC). However, the mechanism of H. pylori-related GC has not been elucidated. This study aimed to explore the role of H. pylori infection in GC and find biomarkers for early diagno...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Biochemistry Research International |
| Online Access: | http://dx.doi.org/10.1155/2020/6285987 |
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| author | Ping Yang Junjie Liu Tianci Yang Lei Zhang Peiyou Gong Boqing Li Xiuzhi Zhou |
| author_facet | Ping Yang Junjie Liu Tianci Yang Lei Zhang Peiyou Gong Boqing Li Xiuzhi Zhou |
| author_sort | Ping Yang |
| collection | DOAJ |
| description | Background. Helicobacter pylori (H. pylori) is a common human pathogen, which is closely correlated with gastric cancer (GC). However, the mechanism of H. pylori-related GC has not been elucidated. This study aimed to explore the role of H. pylori infection in GC and find biomarkers for early diagnosis of H. pylori-related GC. Methods. We identified differentially expressed microRNAs (DEMs) and genes (DEGs) from the Gene Expression Omnibus (GEO) dataset, constructed microRNA-(miRNA-)mRNA expression networks, analyzed the function and signal pathway of cross-genes, analyzed the relations between cross-genes and GC prognosis with the Cancer Genome Atlas (TCGA) data, and verified the expression of cross-genes in patients with H. pylori infection. Results. 22 DEMs and 68 DEGs were identified in GSE197694 and GSE27411 dataset. 16 miRNAs and 509 genes were involved in the expression network, while the cross-genes of the network were mainly enriched in MAP kinase (MAPK) signaling pathway and TGF-beta signaling pathway. Patients with higher expression of hsa-miR-196b-3p, CALML4, or SMAD6 or lower expression of PITX2 or TGFB2 had better outcomes than those with lower expression of hsa-miR-196b-3p, CALML4, or SMAD6 or higher expression of PITX2 or TGFB2 (P<0.05). Patients with H. pylori infection had a higher expression of hsa-miR-196b-3p and CALML4 than those without H. pylori infection (P<0.05). Conclusion. The study of miRNA-mRNA expression network would provide molecular support for early diagnosis and treatment of H. pylori-related GC. |
| format | Article |
| id | doaj-art-3ffdd00542df456dbf4bedd4617f2f0f |
| institution | Kabale University |
| issn | 2090-2247 2090-2255 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Biochemistry Research International |
| spelling | doaj-art-3ffdd00542df456dbf4bedd4617f2f0f2025-02-03T06:06:34ZengWileyBiochemistry Research International2090-22472090-22552020-01-01202010.1155/2020/62859876285987Construction and Investigation of MicroRNA-mRNA Regulatory Network of Gastric Cancer with Helicobacter pylori InfectionPing Yang0Junjie Liu1Tianci Yang2Lei Zhang3Peiyou Gong4Boqing Li5Xiuzhi Zhou6Department of Pathology, Yantai Yuhuangding Hospital of Qingdao University, Yantai 264000, Shandong Province, ChinaSchool of Basic Medical Sciences, Binzhou Medical University, Yantai 264003, Shandong Province, ChinaSchool of Clinical Medicine, Binzhou Medical University, Yantai 264003, Shandong Province, ChinaDepartment of Infectious Diseases, Yantai Affiliated Hospital of Binzhou Medical University, Yantai 264100, Shandong Province, ChinaDepartment of Medicine, Yantai Yuhuangding Hospital of Qingdao University, Yantai 264000, Shandong Province, ChinaSchool of Basic Medical Sciences, Binzhou Medical University, Yantai 264003, Shandong Province, ChinaSchool of Basic Medical Sciences, Binzhou Medical University, Yantai 264003, Shandong Province, ChinaBackground. Helicobacter pylori (H. pylori) is a common human pathogen, which is closely correlated with gastric cancer (GC). However, the mechanism of H. pylori-related GC has not been elucidated. This study aimed to explore the role of H. pylori infection in GC and find biomarkers for early diagnosis of H. pylori-related GC. Methods. We identified differentially expressed microRNAs (DEMs) and genes (DEGs) from the Gene Expression Omnibus (GEO) dataset, constructed microRNA-(miRNA-)mRNA expression networks, analyzed the function and signal pathway of cross-genes, analyzed the relations between cross-genes and GC prognosis with the Cancer Genome Atlas (TCGA) data, and verified the expression of cross-genes in patients with H. pylori infection. Results. 22 DEMs and 68 DEGs were identified in GSE197694 and GSE27411 dataset. 16 miRNAs and 509 genes were involved in the expression network, while the cross-genes of the network were mainly enriched in MAP kinase (MAPK) signaling pathway and TGF-beta signaling pathway. Patients with higher expression of hsa-miR-196b-3p, CALML4, or SMAD6 or lower expression of PITX2 or TGFB2 had better outcomes than those with lower expression of hsa-miR-196b-3p, CALML4, or SMAD6 or higher expression of PITX2 or TGFB2 (P<0.05). Patients with H. pylori infection had a higher expression of hsa-miR-196b-3p and CALML4 than those without H. pylori infection (P<0.05). Conclusion. The study of miRNA-mRNA expression network would provide molecular support for early diagnosis and treatment of H. pylori-related GC.http://dx.doi.org/10.1155/2020/6285987 |
| spellingShingle | Ping Yang Junjie Liu Tianci Yang Lei Zhang Peiyou Gong Boqing Li Xiuzhi Zhou Construction and Investigation of MicroRNA-mRNA Regulatory Network of Gastric Cancer with Helicobacter pylori Infection Biochemistry Research International |
| title | Construction and Investigation of MicroRNA-mRNA Regulatory Network of Gastric Cancer with Helicobacter pylori Infection |
| title_full | Construction and Investigation of MicroRNA-mRNA Regulatory Network of Gastric Cancer with Helicobacter pylori Infection |
| title_fullStr | Construction and Investigation of MicroRNA-mRNA Regulatory Network of Gastric Cancer with Helicobacter pylori Infection |
| title_full_unstemmed | Construction and Investigation of MicroRNA-mRNA Regulatory Network of Gastric Cancer with Helicobacter pylori Infection |
| title_short | Construction and Investigation of MicroRNA-mRNA Regulatory Network of Gastric Cancer with Helicobacter pylori Infection |
| title_sort | construction and investigation of microrna mrna regulatory network of gastric cancer with helicobacter pylori infection |
| url | http://dx.doi.org/10.1155/2020/6285987 |
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