Detection of the Hematopoietic Stem and Progenitor Cell Marker CD133 during Angiogenesis in Three-Dimensional Collagen Gel Culture
We detected the hematopoietic stem and progenitor cell marker CD133 using immunogold labeling during angiogenesis in a three-dimensional collagen gel culture. CD133-positive cells were present in capillary tubes newly formed from aortic explants in vitro. The CD133-positive cell population had the c...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2013/927403 |
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| _version_ | 1832566605283852288 |
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| author | Masumi Akita Kayoko Tanaka Sachiko Matsumoto Kumiko Komatsu Keiko Fujita |
| author_facet | Masumi Akita Kayoko Tanaka Sachiko Matsumoto Kumiko Komatsu Keiko Fujita |
| author_sort | Masumi Akita |
| collection | DOAJ |
| description | We detected the hematopoietic stem and progenitor cell marker CD133 using immunogold labeling during angiogenesis in a three-dimensional collagen gel culture. CD133-positive cells were present in capillary tubes newly formed from aortic explants in vitro. The CD133-positive cell population had the capacity to form capillary tubes. Lovastatin strongly inhibited cell migration from aortic explants and caused the degradation of the capillary tubes. The present study provides insight into the function of CD133 during angiogenesis as well as an explanation for the antiangiogenic effect of statins. |
| format | Article |
| id | doaj-art-3e52ead9fd2940d5a91b736f576bcd9d |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-3e52ead9fd2940d5a91b736f576bcd9d2025-02-03T01:03:35ZengWileyStem Cells International1687-966X1687-96782013-01-01201310.1155/2013/927403927403Detection of the Hematopoietic Stem and Progenitor Cell Marker CD133 during Angiogenesis in Three-Dimensional Collagen Gel CultureMasumi Akita0Kayoko Tanaka1Sachiko Matsumoto2Kumiko Komatsu3Keiko Fujita4Division of Morphological Science, Biomedical Research Center, Saitama Medical University, 38 Moroyama, Iruma-gun, Saitama 350-0495, JapanDivision of Morphological Science, Biomedical Research Center, Saitama Medical University, 38 Moroyama, Iruma-gun, Saitama 350-0495, JapanDivision of Morphological Science, Biomedical Research Center, Saitama Medical University, 38 Moroyama, Iruma-gun, Saitama 350-0495, JapanDivision of Morphological Science, Biomedical Research Center, Saitama Medical University, 38 Moroyama, Iruma-gun, Saitama 350-0495, JapanDepartment of Anatomy, Saitama Medical University, 38 Moroyama, Iruma-gun, Saitama 350-0495, JapanWe detected the hematopoietic stem and progenitor cell marker CD133 using immunogold labeling during angiogenesis in a three-dimensional collagen gel culture. CD133-positive cells were present in capillary tubes newly formed from aortic explants in vitro. The CD133-positive cell population had the capacity to form capillary tubes. Lovastatin strongly inhibited cell migration from aortic explants and caused the degradation of the capillary tubes. The present study provides insight into the function of CD133 during angiogenesis as well as an explanation for the antiangiogenic effect of statins.http://dx.doi.org/10.1155/2013/927403 |
| spellingShingle | Masumi Akita Kayoko Tanaka Sachiko Matsumoto Kumiko Komatsu Keiko Fujita Detection of the Hematopoietic Stem and Progenitor Cell Marker CD133 during Angiogenesis in Three-Dimensional Collagen Gel Culture Stem Cells International |
| title | Detection of the Hematopoietic Stem and Progenitor Cell Marker CD133 during Angiogenesis in Three-Dimensional Collagen Gel Culture |
| title_full | Detection of the Hematopoietic Stem and Progenitor Cell Marker CD133 during Angiogenesis in Three-Dimensional Collagen Gel Culture |
| title_fullStr | Detection of the Hematopoietic Stem and Progenitor Cell Marker CD133 during Angiogenesis in Three-Dimensional Collagen Gel Culture |
| title_full_unstemmed | Detection of the Hematopoietic Stem and Progenitor Cell Marker CD133 during Angiogenesis in Three-Dimensional Collagen Gel Culture |
| title_short | Detection of the Hematopoietic Stem and Progenitor Cell Marker CD133 during Angiogenesis in Three-Dimensional Collagen Gel Culture |
| title_sort | detection of the hematopoietic stem and progenitor cell marker cd133 during angiogenesis in three dimensional collagen gel culture |
| url | http://dx.doi.org/10.1155/2013/927403 |
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