Clostridium tetani bacteraemia in the plague area in France: Two cases
Clostridium tetani (C. tetani) bacteraemia is a rare situation, with only four case reports in the literature. Fourteen teeth from the 1590 plague site in Fédons, France, were surface decontaminated before the pulp was cultured under strict anaerobiosis with negative controls. Colonies were identifi...
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Elsevier
2025-01-01
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| Series: | Current Research in Microbial Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S266651742500001X |
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| author | M.A. Boualam A. Bouri M. Signoli M. Drancourt A. Caputo E. Terrer G. Aboudharam |
| author_facet | M.A. Boualam A. Bouri M. Signoli M. Drancourt A. Caputo E. Terrer G. Aboudharam |
| author_sort | M.A. Boualam |
| collection | DOAJ |
| description | Clostridium tetani (C. tetani) bacteraemia is a rare situation, with only four case reports in the literature. Fourteen teeth from the 1590 plague site in Fédons, France, were surface decontaminated before the pulp was cultured under strict anaerobiosis with negative controls. Colonies were identified by mass spectrometry and whole genome sequencing, and C. tetani-specific PCR was performed using DNA extracted from dental pulps, calculus and sediments. C. tetani cultured in two dental pulp specimens from two individuals was firmly identified by MALDI-TOF mass spectrometry, and whole genome sequencing confirmed toxigenic C. tetani. In the remaining twelve individuals, no such C. tetani was recovered and further detection by PCR and palaeoculturomics of dental calculus and sediments surrounding the teeth in these two individuals remained negative. Toxigenic C. tetani which did not result from mere environmental contamination, caused bacteraemia in two individuals from a modern time plague site in France. |
| format | Article |
| id | doaj-art-3e0b1c01d8684f7c8fdf024b33c45e6d |
| institution | Kabale University |
| issn | 2666-5174 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Microbial Sciences |
| spelling | doaj-art-3e0b1c01d8684f7c8fdf024b33c45e6d2025-01-19T06:26:43ZengElsevierCurrent Research in Microbial Sciences2666-51742025-01-018100339Clostridium tetani bacteraemia in the plague area in France: Two casesM.A. Boualam0A. Bouri1M. Signoli2M. Drancourt3A. Caputo4E. Terrer5G. Aboudharam6Aix-Marseille University, MEPHI, AP-HM, IHU Méditerranée Infection, Marseille, France; IHU Méditerranée Infection, Marseille, FranceAix-Marseille University, MEPHI, AP-HM, IHU Méditerranée Infection, Marseille, France; IHU Méditerranée Infection, Marseille, FranceAix-Marseille University, CNRS, EFS, ADES, UMR, 7268 Marseille, FranceAix-Marseille University, MEPHI, AP-HM, IHU Méditerranée Infection, Marseille, France; IHU Méditerranée Infection, Marseille, FranceAix-Marseille University, MEPHI, AP-HM, IHU Méditerranée Infection, Marseille, FranceAix-Marseille University, MEPHI, AP-HM, IHU Méditerranée Infection, Marseille, France; IHU Méditerranée Infection, Marseille, France; Aix-Marseille University, École de Médecine Dentaire Marseille, FranceAix-Marseille University, MEPHI, AP-HM, IHU Méditerranée Infection, Marseille, France; IHU Méditerranée Infection, Marseille, France; Aix-Marseille University, École de Médecine Dentaire Marseille, France; Corresponding author at: Aix-Marseille University, École de Médecine Dentaire Marseille, France. IRD, MEPHI, AP-HM, IHU Méditerranée Infection, Marseille, France. IHU Méditerranée Infection 19–21 Boulevard Jean Moulin 13005, Marseille, France.Clostridium tetani (C. tetani) bacteraemia is a rare situation, with only four case reports in the literature. Fourteen teeth from the 1590 plague site in Fédons, France, were surface decontaminated before the pulp was cultured under strict anaerobiosis with negative controls. Colonies were identified by mass spectrometry and whole genome sequencing, and C. tetani-specific PCR was performed using DNA extracted from dental pulps, calculus and sediments. C. tetani cultured in two dental pulp specimens from two individuals was firmly identified by MALDI-TOF mass spectrometry, and whole genome sequencing confirmed toxigenic C. tetani. In the remaining twelve individuals, no such C. tetani was recovered and further detection by PCR and palaeoculturomics of dental calculus and sediments surrounding the teeth in these two individuals remained negative. Toxigenic C. tetani which did not result from mere environmental contamination, caused bacteraemia in two individuals from a modern time plague site in France.http://www.sciencedirect.com/science/article/pii/S266651742500001XClostridiumClostridium tetaniTetanusPalaeomicrobiologyPalaeoculturomics |
| spellingShingle | M.A. Boualam A. Bouri M. Signoli M. Drancourt A. Caputo E. Terrer G. Aboudharam Clostridium tetani bacteraemia in the plague area in France: Two cases Current Research in Microbial Sciences Clostridium Clostridium tetani Tetanus Palaeomicrobiology Palaeoculturomics |
| title | Clostridium tetani bacteraemia in the plague area in France: Two cases |
| title_full | Clostridium tetani bacteraemia in the plague area in France: Two cases |
| title_fullStr | Clostridium tetani bacteraemia in the plague area in France: Two cases |
| title_full_unstemmed | Clostridium tetani bacteraemia in the plague area in France: Two cases |
| title_short | Clostridium tetani bacteraemia in the plague area in France: Two cases |
| title_sort | clostridium tetani bacteraemia in the plague area in france two cases |
| topic | Clostridium Clostridium tetani Tetanus Palaeomicrobiology Palaeoculturomics |
| url | http://www.sciencedirect.com/science/article/pii/S266651742500001X |
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