Application of the Gyrolab microfluidic platform to measure picomolar affinity of a PD-L1-binding Adnectin™ radioligand for positron emission tomography

Application of the Gyrolab microfluidic platform to measure picomolar affinity of a PD-L1-binding Adnectin™ radioligand for positron emission tomography

Advances in in vitro display and protein engineering yield therapeutics with affinities in the picomolar range. The Gyrolab® microfluidics platform uses the kinetic exclusion assay principle to measure subnanomolar solution affinities. This work describes application of the Gyrolab solution affinity...

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Bibliographic Details
Main Authors: Zhou Dai, Juhi Juneja, Lumelle Schneeweis, Daniel Cohen, Frank Marsilio, Paul Morin, Ruchira DasGupta
Format: Article
Language:English
Published: Taylor & Francis Group 2020-09-01
Series:BioTechniques
Subjects:
equilibrium dissociation constant (KD)
kinetic exclusion assay
multi-curve analysis
PD-L1-binding Adnectin
signal/background ratio
solution affinity
Online Access:https://www.future-science.com/doi/10.2144/btn-2020-0080
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https://www.future-science.com/doi/10.2144/btn-2020-0080

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