Proteomic analysis of whole blood to investigate the therapeutic effects of nervonic acid on cerebral ischemia-reperfusion injury in rats
IntroductionBlood proteomics offers a powerful approach for identifying disease-specific biomarkers. However, no reliable blood markers are currently available for the diagnosis stroke. Nervonic acid (NA), a vital long-chain monounsaturated fatty acid found in mammalian nervous tissue, shows promisi...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-01-01
|
| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2025.1546073/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832583772472606720 |
|---|---|
| author | Qingqing Li Fengrong Zhang Xianyu Li Qing Wang |
| author_facet | Qingqing Li Fengrong Zhang Xianyu Li Qing Wang |
| author_sort | Qingqing Li |
| collection | DOAJ |
| description | IntroductionBlood proteomics offers a powerful approach for identifying disease-specific biomarkers. However, no reliable blood markers are currently available for the diagnosis stroke. Nervonic acid (NA), a vital long-chain monounsaturated fatty acid found in mammalian nervous tissue, shows promising therapeutic potential in neurological disorders. This study aimed to develop a reliable methodology for whole blood proteomics to identify early warning biomarkers and evaluate drug treatment efficacy.MethodsAfter modeling via the classic thread embolization method, whole blood samples were collected from the rats. Morphological assessments of brain tissue indicated that NA significantly mitigated brain and neuronal damage in rats. The differential protein expression profile was analyzed using Liquid Chromatography—Tandem Mass Spectrometry (LC-MS/MS) whole blood proteomics.ResultsZZZGene Ontology (GO) analysis revealed that, compared to ginkgo biloba extract (EGb), the proteins differentially expressed under NA intervention were predominantly involved in oxidative stress response and calcium-dependent adhesion processes. Key targets of NA in the treatment of middle cerebral artery occlusion (MCAO) models included ENO1, STAT3, NME2, VCL, and CCT3.DiscussionThis whole blood proteomic approach provides a comprehensive understanding of protein profiles associated with disease states, offering valuable insights into potential therapeutic targets and enabling the evaluation of NA and EGb intervention efficacy. Our findings underscore the protective effects of NA against cerebral ischemia-reperfusion injury and highlight its potential as a treatment for stroke. |
| format | Article |
| id | doaj-art-3d2faed7aa1b4984bc0ac55267448dd3 |
| institution | Kabale University |
| issn | 2296-634X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj-art-3d2faed7aa1b4984bc0ac55267448dd32025-01-28T06:41:15ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2025-01-011310.3389/fcell.2025.15460731546073Proteomic analysis of whole blood to investigate the therapeutic effects of nervonic acid on cerebral ischemia-reperfusion injury in ratsQingqing Li0Fengrong Zhang1Xianyu Li2Qing Wang3School of Life Sciences, Beijing University of Chinese Medicine, Beijing, ChinaBeijing Key Laboratory of Traditional Chinese Medicine Basic Research on Prevention and Treatment for Major Diseases, Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing, ChinaBeijing Key Laboratory of Traditional Chinese Medicine Basic Research on Prevention and Treatment for Major Diseases, Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing, ChinaSchool of Life Sciences, Beijing University of Chinese Medicine, Beijing, ChinaIntroductionBlood proteomics offers a powerful approach for identifying disease-specific biomarkers. However, no reliable blood markers are currently available for the diagnosis stroke. Nervonic acid (NA), a vital long-chain monounsaturated fatty acid found in mammalian nervous tissue, shows promising therapeutic potential in neurological disorders. This study aimed to develop a reliable methodology for whole blood proteomics to identify early warning biomarkers and evaluate drug treatment efficacy.MethodsAfter modeling via the classic thread embolization method, whole blood samples were collected from the rats. Morphological assessments of brain tissue indicated that NA significantly mitigated brain and neuronal damage in rats. The differential protein expression profile was analyzed using Liquid Chromatography—Tandem Mass Spectrometry (LC-MS/MS) whole blood proteomics.ResultsZZZGene Ontology (GO) analysis revealed that, compared to ginkgo biloba extract (EGb), the proteins differentially expressed under NA intervention were predominantly involved in oxidative stress response and calcium-dependent adhesion processes. Key targets of NA in the treatment of middle cerebral artery occlusion (MCAO) models included ENO1, STAT3, NME2, VCL, and CCT3.DiscussionThis whole blood proteomic approach provides a comprehensive understanding of protein profiles associated with disease states, offering valuable insights into potential therapeutic targets and enabling the evaluation of NA and EGb intervention efficacy. Our findings underscore the protective effects of NA against cerebral ischemia-reperfusion injury and highlight its potential as a treatment for stroke.https://www.frontiersin.org/articles/10.3389/fcell.2025.1546073/fullwhole blood proteomecerebral ischemia-reperfusion injurynervonic acidginkgo biloba extractmechanism |
| spellingShingle | Qingqing Li Fengrong Zhang Xianyu Li Qing Wang Proteomic analysis of whole blood to investigate the therapeutic effects of nervonic acid on cerebral ischemia-reperfusion injury in rats Frontiers in Cell and Developmental Biology whole blood proteome cerebral ischemia-reperfusion injury nervonic acid ginkgo biloba extract mechanism |
| title | Proteomic analysis of whole blood to investigate the therapeutic effects of nervonic acid on cerebral ischemia-reperfusion injury in rats |
| title_full | Proteomic analysis of whole blood to investigate the therapeutic effects of nervonic acid on cerebral ischemia-reperfusion injury in rats |
| title_fullStr | Proteomic analysis of whole blood to investigate the therapeutic effects of nervonic acid on cerebral ischemia-reperfusion injury in rats |
| title_full_unstemmed | Proteomic analysis of whole blood to investigate the therapeutic effects of nervonic acid on cerebral ischemia-reperfusion injury in rats |
| title_short | Proteomic analysis of whole blood to investigate the therapeutic effects of nervonic acid on cerebral ischemia-reperfusion injury in rats |
| title_sort | proteomic analysis of whole blood to investigate the therapeutic effects of nervonic acid on cerebral ischemia reperfusion injury in rats |
| topic | whole blood proteome cerebral ischemia-reperfusion injury nervonic acid ginkgo biloba extract mechanism |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2025.1546073/full |
| work_keys_str_mv | AT qingqingli proteomicanalysisofwholebloodtoinvestigatethetherapeuticeffectsofnervonicacidoncerebralischemiareperfusioninjuryinrats AT fengrongzhang proteomicanalysisofwholebloodtoinvestigatethetherapeuticeffectsofnervonicacidoncerebralischemiareperfusioninjuryinrats AT xianyuli proteomicanalysisofwholebloodtoinvestigatethetherapeuticeffectsofnervonicacidoncerebralischemiareperfusioninjuryinrats AT qingwang proteomicanalysisofwholebloodtoinvestigatethetherapeuticeffectsofnervonicacidoncerebralischemiareperfusioninjuryinrats |