A SH3b domain-containing peptidoglycan recognition protein from Magallana gigas with broad recognition spectrum
Peptidoglycan recognition protein (PGRP) is a kind of conserved pattern recognition receptors (PRRs) and plays important roles in innate immune response in both invertebrates and vertebrates. In the present study, nine MgPGRP genes were identified in the updated genome of Magallana gigas, and the cD...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-06-01
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| Series: | Comparative Immunology Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2950311625000084 |
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| Summary: | Peptidoglycan recognition protein (PGRP) is a kind of conserved pattern recognition receptors (PRRs) and plays important roles in innate immune response in both invertebrates and vertebrates. In the present study, nine MgPGRP genes were identified in the updated genome of Magallana gigas, and the cDNA of MgPGRP5 which was consisted of a SH3b and a PGRP domain was cloned. The mRNA transcripts of MgPGRP5 were constitutively expressed in haemocytes and all the examined tissues, with the highest expression level in hepatopancreas. Both PGN and LPS stimulation could significantly up-regulated the expression level of MgPGRP5 mRNA in haemocytes (p < 0.05). Two amino acids in MgPGRP5, Asp230 and Glu106, were identified as potential binding sites for PGN and LPS. The MgPGRP5 protein was mainly located in nucleus of haemocytes, and showed widespread expression in hepatopancreas. The recombinant MgPGRP5 protein (rMgPGRP5) displayed strong binding and agglutinated activities to Gram-negative bacteria, Gram-positive bacteria and fungi. These results indicated that MgPGRP5 could function as PRR with a broad range of recognition spectrum in the innate immune response of oyster. |
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| ISSN: | 2950-3116 |