Beneficial Effects of Long-Term Administration of an Oral Formulation of Angiotensin-(1–7) in Infarcted Rats
In this study was evaluated the chronic cardiac effects of a formulation developed by including angiotensin(Ang)-(1–7) in hydroxypropyl β-cyclodextrin (HPβCD), in infarcted rats. Myocardial infarction (MI) was induced by left coronary artery occlusion. HPβCD/Ang-(1–7) was administered for 60 days (7...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | International Journal of Hypertension |
| Online Access: | http://dx.doi.org/10.1155/2012/795452 |
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| author | Fúlvia D. Marques Marcos B. Melo Leandro E. Souza Maria Claúdia C. Irigoyen Rúben D. Sinisterra Frederico B. de Sousa Sílvia Q. Savergnini Vinícius B. A. Braga Anderson J. Ferreira Robson A. S. Santos |
| author_facet | Fúlvia D. Marques Marcos B. Melo Leandro E. Souza Maria Claúdia C. Irigoyen Rúben D. Sinisterra Frederico B. de Sousa Sílvia Q. Savergnini Vinícius B. A. Braga Anderson J. Ferreira Robson A. S. Santos |
| author_sort | Fúlvia D. Marques |
| collection | DOAJ |
| description | In this study was evaluated the chronic cardiac effects of a formulation developed by including angiotensin(Ang)-(1–7) in hydroxypropyl β-cyclodextrin (HPβCD), in infarcted rats. Myocardial infarction (MI) was induced by left coronary artery occlusion. HPβCD/Ang-(1–7) was administered for 60 days (76 μg/Kg/once a day/gavage) starting immediately before infarction. Echocardiography was utilized to evaluate usual cardiac parameters, and radial strain method was used to analyze the velocity and displacement of myocardial fibers at initial time and 15, 30, and 50 days after surgery. Real-time PCR was utilized to evaluate the fibrotic signaling involved in the remodeling process. Once-a-day oral HPβCD/Ang-(1–7) administration improved the cardiac function and reduced the deleterious effects induced by MI on TGF-β and collagen type I expression, as well as on the velocity and displacement of myocardial fibers. These findings confirm cardioprotective effects of Ang-(1–7) and indicate HPβCD/Ang-(1–7) as a feasible formulation for long-term oral administration of this heptapeptide. |
| format | Article |
| id | doaj-art-3ccaabce7df040ccbcf7fcb7c6ced107 |
| institution | Kabale University |
| issn | 2090-0384 2090-0392 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Hypertension |
| spelling | doaj-art-3ccaabce7df040ccbcf7fcb7c6ced1072025-02-03T01:20:13ZengWileyInternational Journal of Hypertension2090-03842090-03922012-01-01201210.1155/2012/795452795452Beneficial Effects of Long-Term Administration of an Oral Formulation of Angiotensin-(1–7) in Infarcted RatsFúlvia D. Marques0Marcos B. Melo1Leandro E. Souza2Maria Claúdia C. Irigoyen3Rúben D. Sinisterra4Frederico B. de Sousa5Sílvia Q. Savergnini6Vinícius B. A. Braga7Anderson J. Ferreira8Robson A. S. Santos9National Institute of Science and Technology in Nanobiopharmaceutics, Federal University of Minas Gerais, 31.270-901 Belo Horizonte, MG, BrazilNational Institute of Science and Technology in Nanobiopharmaceutics, Federal University of Minas Gerais, 31.270-901 Belo Horizonte, MG, BrazilNational Institute of Science and Technology in Nanobiopharmaceutics, Federal University of Minas Gerais, 31.270-901 Belo Horizonte, MG, BrazilNational Institute of Science and Technology in Nanobiopharmaceutics, Federal University of Minas Gerais, 31.270-901 Belo Horizonte, MG, BrazilNational Institute of Science and Technology in Nanobiopharmaceutics, Federal University of Minas Gerais, 31.270-901 Belo Horizonte, MG, BrazilNational Institute of Science and Technology in Nanobiopharmaceutics, Federal University of Minas Gerais, 31.270-901 Belo Horizonte, MG, BrazilNational Institute of Science and Technology in Nanobiopharmaceutics, Federal University of Minas Gerais, 31.270-901 Belo Horizonte, MG, BrazilDepartment of Morphology, Federal University of Minas Gerais, 31.270-901 Belo Horizonte, MG, BrazilNational Institute of Science and Technology in Nanobiopharmaceutics, Federal University of Minas Gerais, 31.270-901 Belo Horizonte, MG, BrazilNational Institute of Science and Technology in Nanobiopharmaceutics, Federal University of Minas Gerais, 31.270-901 Belo Horizonte, MG, BrazilIn this study was evaluated the chronic cardiac effects of a formulation developed by including angiotensin(Ang)-(1–7) in hydroxypropyl β-cyclodextrin (HPβCD), in infarcted rats. Myocardial infarction (MI) was induced by left coronary artery occlusion. HPβCD/Ang-(1–7) was administered for 60 days (76 μg/Kg/once a day/gavage) starting immediately before infarction. Echocardiography was utilized to evaluate usual cardiac parameters, and radial strain method was used to analyze the velocity and displacement of myocardial fibers at initial time and 15, 30, and 50 days after surgery. Real-time PCR was utilized to evaluate the fibrotic signaling involved in the remodeling process. Once-a-day oral HPβCD/Ang-(1–7) administration improved the cardiac function and reduced the deleterious effects induced by MI on TGF-β and collagen type I expression, as well as on the velocity and displacement of myocardial fibers. These findings confirm cardioprotective effects of Ang-(1–7) and indicate HPβCD/Ang-(1–7) as a feasible formulation for long-term oral administration of this heptapeptide.http://dx.doi.org/10.1155/2012/795452 |
| spellingShingle | Fúlvia D. Marques Marcos B. Melo Leandro E. Souza Maria Claúdia C. Irigoyen Rúben D. Sinisterra Frederico B. de Sousa Sílvia Q. Savergnini Vinícius B. A. Braga Anderson J. Ferreira Robson A. S. Santos Beneficial Effects of Long-Term Administration of an Oral Formulation of Angiotensin-(1–7) in Infarcted Rats International Journal of Hypertension |
| title | Beneficial Effects of Long-Term Administration of an Oral Formulation of Angiotensin-(1–7) in Infarcted Rats |
| title_full | Beneficial Effects of Long-Term Administration of an Oral Formulation of Angiotensin-(1–7) in Infarcted Rats |
| title_fullStr | Beneficial Effects of Long-Term Administration of an Oral Formulation of Angiotensin-(1–7) in Infarcted Rats |
| title_full_unstemmed | Beneficial Effects of Long-Term Administration of an Oral Formulation of Angiotensin-(1–7) in Infarcted Rats |
| title_short | Beneficial Effects of Long-Term Administration of an Oral Formulation of Angiotensin-(1–7) in Infarcted Rats |
| title_sort | beneficial effects of long term administration of an oral formulation of angiotensin 1 7 in infarcted rats |
| url | http://dx.doi.org/10.1155/2012/795452 |
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