A human oral commensal-mediated protection against Sjögren’s syndrome with maintenance of T cell immune homeostasis and improved oral microbiota
Abstract Sjögren’s syndrome (SS) is a prevalent systemic autoimmune disease with substantial impacts on women’s health worldwide. Although oral Haemophilus parainfluenzae is reduced in SS, its significance remains unclear. This study aimed to elucidate the pathophysiological role of H. parainfluenza...
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Nature Portfolio
2025-01-01
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| Series: | npj Biofilms and Microbiomes |
| Online Access: | https://doi.org/10.1038/s41522-025-00654-5 |
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| author | Yu-Chao Tseng Kai-Sheng Liao Wei-ting Lin Chin Li Chia-Bin Chang Jie-Wei Hsu Chin-Pui Chan Chun-Ming Chen Hon-Pin Wang Hsiu-Chuan Chien Jann-Tay Wang Song-Chou Hsieh Shu-Fen Wu |
| author_facet | Yu-Chao Tseng Kai-Sheng Liao Wei-ting Lin Chin Li Chia-Bin Chang Jie-Wei Hsu Chin-Pui Chan Chun-Ming Chen Hon-Pin Wang Hsiu-Chuan Chien Jann-Tay Wang Song-Chou Hsieh Shu-Fen Wu |
| author_sort | Yu-Chao Tseng |
| collection | DOAJ |
| description | Abstract Sjögren’s syndrome (SS) is a prevalent systemic autoimmune disease with substantial impacts on women’s health worldwide. Although oral Haemophilus parainfluenzae is reduced in SS, its significance remains unclear. This study aimed to elucidate the pathophysiological role of H. parainfluenzae in SS. Reduced salivary H. parainfluenzae levels in SS patients were confirmed through quantitative PCR. Oral H. parainfluenzae inoculation in NOD mice alleviated focal sialadenitis, improved salivary function, and reduced IFN-γ+CD3+ and IFN-γ+CD8+ T cells in salivary gland-draining lymph nodes, maintaining immune homeostasis against a biased type 1 response. Inoculation also enhanced salivary microbiota diversity, balanced the Firmicutes-to-Proteobacteria ratio, and reduced the overwhelming presence of Pseudomonas mendocina. In vitro, H. parainfluenzae-preconditioned A253 cells limited CD8 T cell expansion with reduced IFN-γ production. These findings suggest that H. parainfluenzae improves oral microbial diversity, promotes homeostatic T-cell immunity, and protects against SS, supporting its potential as a next-generation probiotic. |
| format | Article |
| id | doaj-art-3c5ba869c31c4bb9abdac2705e9fda79 |
| institution | Kabale University |
| issn | 2055-5008 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Biofilms and Microbiomes |
| spelling | doaj-art-3c5ba869c31c4bb9abdac2705e9fda792025-01-19T12:12:18ZengNature Portfolionpj Biofilms and Microbiomes2055-50082025-01-0111111210.1038/s41522-025-00654-5A human oral commensal-mediated protection against Sjögren’s syndrome with maintenance of T cell immune homeostasis and improved oral microbiotaYu-Chao Tseng0Kai-Sheng Liao1Wei-ting Lin2Chin Li3Chia-Bin Chang4Jie-Wei Hsu5Chin-Pui Chan6Chun-Ming Chen7Hon-Pin Wang8Hsiu-Chuan Chien9Jann-Tay Wang10Song-Chou Hsieh11Shu-Fen Wu12Division of Allergy, Immunology, and Rheumatology, Department of Internal Medicine, Ditmanson Medical Foundation Chia-Yi Christian HospitalDepartment of Pathology, Ditmanson Medical Foundation Chia-Yi Christian HospitalDepartment Oral and Maxillofacial Surgery, Ditmanson Medical Foundation Chia-Yi Christian HospitalDepartment of Biomedical Sciences and Institute of Molecular Biology, National Chung Cheng UniversityDepartment of Urology, Ditmanson Medical Foundation Chia-Yi Christian HospitalDepartment of Biomedical Sciences and Institute of Molecular Biology, National Chung Cheng UniversityDepartment of Biomedical Sciences and Institute of Molecular Biology, National Chung Cheng UniversityDivision of Allergy, Immunology, and Rheumatology, Department of Internal Medicine, Ditmanson Medical Foundation Chia-Yi Christian HospitalDivision of Allergy, Immunology, and Rheumatology, Department of Internal Medicine, Ditmanson Medical Foundation Chia-Yi Christian HospitalDepartment of Laboratory Medicine, Ditmanson Medical Foundation Chia-Yi Christian HospitalDepartment of Internal Medicine, National Taiwan University HospitalDepartment of Internal Medicine, National Taiwan University HospitalDepartment of Biomedical Sciences and Institute of Molecular Biology, National Chung Cheng UniversityAbstract Sjögren’s syndrome (SS) is a prevalent systemic autoimmune disease with substantial impacts on women’s health worldwide. Although oral Haemophilus parainfluenzae is reduced in SS, its significance remains unclear. This study aimed to elucidate the pathophysiological role of H. parainfluenzae in SS. Reduced salivary H. parainfluenzae levels in SS patients were confirmed through quantitative PCR. Oral H. parainfluenzae inoculation in NOD mice alleviated focal sialadenitis, improved salivary function, and reduced IFN-γ+CD3+ and IFN-γ+CD8+ T cells in salivary gland-draining lymph nodes, maintaining immune homeostasis against a biased type 1 response. Inoculation also enhanced salivary microbiota diversity, balanced the Firmicutes-to-Proteobacteria ratio, and reduced the overwhelming presence of Pseudomonas mendocina. In vitro, H. parainfluenzae-preconditioned A253 cells limited CD8 T cell expansion with reduced IFN-γ production. These findings suggest that H. parainfluenzae improves oral microbial diversity, promotes homeostatic T-cell immunity, and protects against SS, supporting its potential as a next-generation probiotic.https://doi.org/10.1038/s41522-025-00654-5 |
| spellingShingle | Yu-Chao Tseng Kai-Sheng Liao Wei-ting Lin Chin Li Chia-Bin Chang Jie-Wei Hsu Chin-Pui Chan Chun-Ming Chen Hon-Pin Wang Hsiu-Chuan Chien Jann-Tay Wang Song-Chou Hsieh Shu-Fen Wu A human oral commensal-mediated protection against Sjögren’s syndrome with maintenance of T cell immune homeostasis and improved oral microbiota npj Biofilms and Microbiomes |
| title | A human oral commensal-mediated protection against Sjögren’s syndrome with maintenance of T cell immune homeostasis and improved oral microbiota |
| title_full | A human oral commensal-mediated protection against Sjögren’s syndrome with maintenance of T cell immune homeostasis and improved oral microbiota |
| title_fullStr | A human oral commensal-mediated protection against Sjögren’s syndrome with maintenance of T cell immune homeostasis and improved oral microbiota |
| title_full_unstemmed | A human oral commensal-mediated protection against Sjögren’s syndrome with maintenance of T cell immune homeostasis and improved oral microbiota |
| title_short | A human oral commensal-mediated protection against Sjögren’s syndrome with maintenance of T cell immune homeostasis and improved oral microbiota |
| title_sort | human oral commensal mediated protection against sjogren s syndrome with maintenance of t cell immune homeostasis and improved oral microbiota |
| url | https://doi.org/10.1038/s41522-025-00654-5 |
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