Evaluation of methylene blue restaining versus conventional hydrogen peroxide decolorization in immunohistochemical diagnosis of melanoma

Abstract This study seeks to address the challenge of melanoma identification in immunohistochemical (IHC) diagnosis, which is complicated by the similar coloration of melanin and DAB (Diaminobenzidine) staining, by introducing methylene blue counterstaining as an innovative solution. We compared th...

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Bibliographic Details
Main Authors: Weisong Wan, Le Wang, Yuye Zeng, Yuchang Hu, Yufei Liu
Format: Article
Language:English
Published: Nature Portfolio 2025-02-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-89186-8
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