An anatomical aspect on masseteric muscles in cattle rabies by real-time pcr
<b>Aim:</b> In this case, the anatomical pathways on masticatory muscles of cattle infected rabies virüs have been determined by Real Time PCR (RT-PCR).<p> <b>Materials and Methods:</b> A 1.5-year old male Brown Swiss cattle which was bitten in face by dog was used. The...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1016 |
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| Summary: | <b>Aim:</b> In this case, the anatomical pathways on masticatory
muscles of cattle infected rabies virüs have been determined
by Real Time PCR (RT-PCR).<p>
<b>Materials and Methods:</b> A 1.5-year old male Brown Swiss
cattle which was bitten in face by dog was used. The cattle
was died 15 days post exposure to the dog rabies suspected.
The areas where the tissue samples to taken. The origin,
course and branches of the trigeminal nerve were exposed
by standart dissection method. Rabies virus was investigated
on 32 different parts of the cattle's head by means of TaqMan
Probe-Based Real-Time PCR and FAT.<p>
<b>Results:</b> The rabies virus specific nucleic acid was detected
in masseter muscles motor nerves; N. mandibularis branch
of N. trigeminus, N. masticatorius, motor leaf of N. mandibularis,
N. massetericus and Nn. temporalis profunda which are
two extra branches of N. masticatorius, unilaterally ganglion
trigeminale, and pons by Real Time-PCR. However, no rabies
virus has been detected in the samples obtained from other
different parts of the brain.<p>
<b>Conclusion:</b> Contrary to immunohistochemical methods, it
is not possible to trace neuronal connections across synapses
within the brain. RT-PCR method could be helpful to detect
pathways of the neurotropic agents suspected cattle. |
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| ISSN: | 1309-6958 2146-1953 |