Effect of partial oocyte maturation before ovum pick up on developmental competence of buffalo oocytes
Compared with in vivo matured oocytes, in vitro maturation produces half the number of blastocysts in cattle. We hypothesized that partial maturation of oocytes in vivo improves blastocyst formation in buffalo. Therefore, we aimed to investigate the effect of partial in vivo maturation on in vitro...
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Universidad del Zulia
2023-11-01
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| Series: | Revista Científica |
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| author | Amjad Riaz Muhammad K. Ashraf Talha Ashraf Ali Husnain Muhammad Yaseen Muhammad H. Mushtaq Burhan E. Azam |
| author_facet | Amjad Riaz Muhammad K. Ashraf Talha Ashraf Ali Husnain Muhammad Yaseen Muhammad H. Mushtaq Burhan E. Azam |
| author_sort | Amjad Riaz |
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Compared with in vivo matured oocytes, in vitro maturation produces half the number of blastocysts in cattle. We hypothesized that partial maturation of oocytes in vivo improves blastocyst formation in buffalo. Therefore, we aimed to investigate the effect of partial in vivo maturation on in vitro embryo production (IVEP). The estrous cycle of buffalo was synchronized by double shot prostaglandin (PGF2α, Fatro, Ozzano Emilia, Italy) with 11-day intervals. On the last PGF2α, dominant follicle ablation and supplementation of progesterone by inserting intra- vaginal controlled internal drug release was performed to synchronize the follicle wave emergence and considered as Day 0 of the experiment. Starting on Day 2 of the experiment, the ovaries of buffalo were super- stimulated with six equally divided doses of 33.3 mg per dose of porcine follicle stimulating hormone (Vetoquinol, Magny-Vernois, France) i.m., for three days with 12 h interval. The experiment was randomized, with a complete block design. On Day 5 of the experiment, buffaloes were blocked by parity, and within the block, they were assigned randomly to receive 1500 IU of human chorionic gonadotropin i.m., (hCG, LG Chem, Seoul, South Korea; n = 6) or to remain untreated control (CON; n = 4). Oocytes were aspirated six hours after treatment implementations using ultrasound-guided Ovum Pick Up (OPU). The data was analyzed by using mixed effect models in SAS statistical software. Treatment with hCG did not affect the number of small, medium, and large follicles at the time of OPU. However, hCG treatment increased (p< 0.01) cleaved oocytes compared with CON (84.0 vs. 40.5 ± 7.2%). Similarly, oocytes treated with hCG had greater (p = 0.04) development from cultured oocytes to Day 7 embryo (52.5 vs. 18.1 ± 10.3%). Treatment with hCG did not affect the development of cleaved oocytes to Day 7 embryo. In conclusion, our results indicated that partial oocyte maturation improved the in vitro embryo production. However, the impact was pronounced at the cleavage rate rather than in the later stages of embryo development.
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| format | Article |
| id | doaj-art-3c19b5e4da0f4f56ab6134ecc4d3352c |
| institution | Kabale University |
| issn | 0798-2259 2521-9715 |
| language | English |
| publishDate | 2023-11-01 |
| publisher | Universidad del Zulia |
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| series | Revista Científica |
| spelling | doaj-art-3c19b5e4da0f4f56ab6134ecc4d3352c2025-02-06T15:37:10ZengUniversidad del ZuliaRevista Científica0798-22592521-97152023-11-0133Suplemento10.52973/rcfcv-wbc120Effect of partial oocyte maturation before ovum pick up on developmental competence of buffalo oocytesAmjad Riaz0Muhammad K. Ashraf1Talha Ashraf2Ali Husnain3Muhammad Yaseen 4Muhammad H. Mushtaq5Burhan E. Azam6Department of Theriogenology. PakistánDepartment of Theriogenology. ParkistánDepartment of Theriogenology. PakistánDepartment of Theriogenology. PakistánDepartment of Theriogenology. PakistánDepartment of Epidemiology & Public Health, University of Veterinary and Animal Sciences, Lahore, Pakistan.Livestock Experimental Station Bhunikey, Buffalo Research Institute, Pattoki, Pakistan Compared with in vivo matured oocytes, in vitro maturation produces half the number of blastocysts in cattle. We hypothesized that partial maturation of oocytes in vivo improves blastocyst formation in buffalo. Therefore, we aimed to investigate the effect of partial in vivo maturation on in vitro embryo production (IVEP). The estrous cycle of buffalo was synchronized by double shot prostaglandin (PGF2α, Fatro, Ozzano Emilia, Italy) with 11-day intervals. On the last PGF2α, dominant follicle ablation and supplementation of progesterone by inserting intra- vaginal controlled internal drug release was performed to synchronize the follicle wave emergence and considered as Day 0 of the experiment. Starting on Day 2 of the experiment, the ovaries of buffalo were super- stimulated with six equally divided doses of 33.3 mg per dose of porcine follicle stimulating hormone (Vetoquinol, Magny-Vernois, France) i.m., for three days with 12 h interval. The experiment was randomized, with a complete block design. On Day 5 of the experiment, buffaloes were blocked by parity, and within the block, they were assigned randomly to receive 1500 IU of human chorionic gonadotropin i.m., (hCG, LG Chem, Seoul, South Korea; n = 6) or to remain untreated control (CON; n = 4). Oocytes were aspirated six hours after treatment implementations using ultrasound-guided Ovum Pick Up (OPU). The data was analyzed by using mixed effect models in SAS statistical software. Treatment with hCG did not affect the number of small, medium, and large follicles at the time of OPU. However, hCG treatment increased (p< 0.01) cleaved oocytes compared with CON (84.0 vs. 40.5 ± 7.2%). Similarly, oocytes treated with hCG had greater (p = 0.04) development from cultured oocytes to Day 7 embryo (52.5 vs. 18.1 ± 10.3%). Treatment with hCG did not affect the development of cleaved oocytes to Day 7 embryo. In conclusion, our results indicated that partial oocyte maturation improved the in vitro embryo production. However, the impact was pronounced at the cleavage rate rather than in the later stages of embryo development. https://mail.produccioncientificaluz.org/index.php/cientifica/article/view/43472buffaloovum pick upin vivo maturationhCGcleavage |
| spellingShingle | Amjad Riaz Muhammad K. Ashraf Talha Ashraf Ali Husnain Muhammad Yaseen Muhammad H. Mushtaq Burhan E. Azam Effect of partial oocyte maturation before ovum pick up on developmental competence of buffalo oocytes Revista Científica buffalo ovum pick up in vivo maturation hCG cleavage |
| title | Effect of partial oocyte maturation before ovum pick up on developmental competence of buffalo oocytes |
| title_full | Effect of partial oocyte maturation before ovum pick up on developmental competence of buffalo oocytes |
| title_fullStr | Effect of partial oocyte maturation before ovum pick up on developmental competence of buffalo oocytes |
| title_full_unstemmed | Effect of partial oocyte maturation before ovum pick up on developmental competence of buffalo oocytes |
| title_short | Effect of partial oocyte maturation before ovum pick up on developmental competence of buffalo oocytes |
| title_sort | effect of partial oocyte maturation before ovum pick up on developmental competence of buffalo oocytes |
| topic | buffalo ovum pick up in vivo maturation hCG cleavage |
| url | https://mail.produccioncientificaluz.org/index.php/cientifica/article/view/43472 |
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