Leptin Regulates Proliferation and Apoptosis in Human Prostate
This paper aimed to evaluate the leptin role on the cellular proliferation and the expression of fibroblast growth factor 2, aromatase enzyme, and apoptotic genes in the human prostate tissue. Methods. Fifteen samples of hyperplasic prostate tissue were divided in four symmetric parts maintained in...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/842301 |
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| author | Eduardo Leze Jorge Luiz Alves-Pereira Sicilia Colli Fernanda Silveira Cavalcante Francisco José Sampaio Cristiane da Fonte Ramos |
| author_facet | Eduardo Leze Jorge Luiz Alves-Pereira Sicilia Colli Fernanda Silveira Cavalcante Francisco José Sampaio Cristiane da Fonte Ramos |
| author_sort | Eduardo Leze |
| collection | DOAJ |
| description | This paper aimed to evaluate the leptin role on the cellular proliferation and the expression of fibroblast growth factor 2, aromatase enzyme, and apoptotic genes in the human prostate tissue. Methods. Fifteen samples of hyperplasic prostate tissue were divided in four symmetric parts maintained in RPMI medium supplemented with 10% fetal bovine serum, 1 ng/mL of gentamicin, and added with 50 ng/mL leptin (L) or not (C). After 3 hours of incubation, gene expression was evaluated by real time RT-PCR. Cellular proliferation was evaluated by immunohistochemistry for PCNA. Results. The leptin treatment led to an increase cellular proliferation (C=21.8±0.5; L=64.8±0.9; P<0.0001) and in the expression of Bax (C=0.4±0.1; L=0.9±0.2; P<0.05) while Bcl-2 (C=19.9±5.6; L=5.6±1.8; P<0.05), Bcl-x (C=0.2±0.06; L=0.07±0.02; P<0.05), and aromatase expressions (C=1.9±0.6; L=0.4±0.1; P<0.04) were significantly reduced. Conclusion. Leptin has an important role in maintaining the physiological growth of the prostate since it stimulates both cellular proliferation and apoptosis, with the decrement in the aromatase gene expression. |
| format | Article |
| id | doaj-art-3b82dd3e4e114d88a7b0b0068e0aab04 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-3b82dd3e4e114d88a7b0b0068e0aab042025-02-03T06:04:56ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/842301842301Leptin Regulates Proliferation and Apoptosis in Human ProstateEduardo Leze0Jorge Luiz Alves-Pereira1Sicilia Colli2Fernanda Silveira Cavalcante3Francisco José Sampaio4Cristiane da Fonte Ramos5Urogenital Research Unit, State University of Rio de Janeiro, 20551-030 Rio de Janeiro, RJ, BrazilUrogenital Research Unit, State University of Rio de Janeiro, 20551-030 Rio de Janeiro, RJ, BrazilUrogenital Research Unit, State University of Rio de Janeiro, 20551-030 Rio de Janeiro, RJ, BrazilUrogenital Research Unit, State University of Rio de Janeiro, 20551-030 Rio de Janeiro, RJ, BrazilUrogenital Research Unit, State University of Rio de Janeiro, 20551-030 Rio de Janeiro, RJ, BrazilUrogenital Research Unit, State University of Rio de Janeiro, 20551-030 Rio de Janeiro, RJ, BrazilThis paper aimed to evaluate the leptin role on the cellular proliferation and the expression of fibroblast growth factor 2, aromatase enzyme, and apoptotic genes in the human prostate tissue. Methods. Fifteen samples of hyperplasic prostate tissue were divided in four symmetric parts maintained in RPMI medium supplemented with 10% fetal bovine serum, 1 ng/mL of gentamicin, and added with 50 ng/mL leptin (L) or not (C). After 3 hours of incubation, gene expression was evaluated by real time RT-PCR. Cellular proliferation was evaluated by immunohistochemistry for PCNA. Results. The leptin treatment led to an increase cellular proliferation (C=21.8±0.5; L=64.8±0.9; P<0.0001) and in the expression of Bax (C=0.4±0.1; L=0.9±0.2; P<0.05) while Bcl-2 (C=19.9±5.6; L=5.6±1.8; P<0.05), Bcl-x (C=0.2±0.06; L=0.07±0.02; P<0.05), and aromatase expressions (C=1.9±0.6; L=0.4±0.1; P<0.04) were significantly reduced. Conclusion. Leptin has an important role in maintaining the physiological growth of the prostate since it stimulates both cellular proliferation and apoptosis, with the decrement in the aromatase gene expression.http://dx.doi.org/10.1100/2012/842301 |
| spellingShingle | Eduardo Leze Jorge Luiz Alves-Pereira Sicilia Colli Fernanda Silveira Cavalcante Francisco José Sampaio Cristiane da Fonte Ramos Leptin Regulates Proliferation and Apoptosis in Human Prostate The Scientific World Journal |
| title | Leptin Regulates Proliferation and Apoptosis in Human Prostate |
| title_full | Leptin Regulates Proliferation and Apoptosis in Human Prostate |
| title_fullStr | Leptin Regulates Proliferation and Apoptosis in Human Prostate |
| title_full_unstemmed | Leptin Regulates Proliferation and Apoptosis in Human Prostate |
| title_short | Leptin Regulates Proliferation and Apoptosis in Human Prostate |
| title_sort | leptin regulates proliferation and apoptosis in human prostate |
| url | http://dx.doi.org/10.1100/2012/842301 |
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