HPLC Quantification of Cytotoxic Compounds from Aspergillus niger
A high-performance liquid chromatography method was developed and validated for the quantification of the cytotoxic compounds produced by a marine strain of Aspergillus niger. The fungus was grown in malt peptone dextrose (MPD), potato dextrose yeast (PDY), and mannitol peptone yeast (MnPY) media du...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2017/6969358 |
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| author | Paula Karina S. Uchoa Leandro Bezerra de Lima Antonia T. A. Pimenta Maria da Conceição F. de Oliveira Jair Mafezoli Mary Anne S. Lima |
| author_facet | Paula Karina S. Uchoa Leandro Bezerra de Lima Antonia T. A. Pimenta Maria da Conceição F. de Oliveira Jair Mafezoli Mary Anne S. Lima |
| author_sort | Paula Karina S. Uchoa |
| collection | DOAJ |
| description | A high-performance liquid chromatography method was developed and validated for the quantification of the cytotoxic compounds produced by a marine strain of Aspergillus niger. The fungus was grown in malt peptone dextrose (MPD), potato dextrose yeast (PDY), and mannitol peptone yeast (MnPY) media during 7, 14, 21, and 28 days, and the natural products were identified by standard compounds. The validation parameters obtained were selectivity, linearity (coefficient of correlation > 0.99), precision (relative standard deviation below 5%), and accuracy (recovery > 96). |
| format | Article |
| id | doaj-art-3b1a070de29e4e9b9bd0d89dd166b1c7 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-3b1a070de29e4e9b9bd0d89dd166b1c72025-02-03T05:45:42ZengWileyJournal of Chemistry2090-90632090-90712017-01-01201710.1155/2017/69693586969358HPLC Quantification of Cytotoxic Compounds from Aspergillus nigerPaula Karina S. Uchoa0Leandro Bezerra de Lima1Antonia T. A. Pimenta2Maria da Conceição F. de Oliveira3Jair Mafezoli4Mary Anne S. Lima5Departamento de Química Orgânica e Inorgânica, Centro de Ciências, Universidade Federal do Ceará, Fortaleza, CE, BrazilDepartamento de Química Orgânica e Inorgânica, Centro de Ciências, Universidade Federal do Ceará, Fortaleza, CE, BrazilDepartamento de Química Orgânica e Inorgânica, Centro de Ciências, Universidade Federal do Ceará, Fortaleza, CE, BrazilDepartamento de Química Orgânica e Inorgânica, Centro de Ciências, Universidade Federal do Ceará, Fortaleza, CE, BrazilDepartamento de Química Orgânica e Inorgânica, Centro de Ciências, Universidade Federal do Ceará, Fortaleza, CE, BrazilDepartamento de Química Orgânica e Inorgânica, Centro de Ciências, Universidade Federal do Ceará, Fortaleza, CE, BrazilA high-performance liquid chromatography method was developed and validated for the quantification of the cytotoxic compounds produced by a marine strain of Aspergillus niger. The fungus was grown in malt peptone dextrose (MPD), potato dextrose yeast (PDY), and mannitol peptone yeast (MnPY) media during 7, 14, 21, and 28 days, and the natural products were identified by standard compounds. The validation parameters obtained were selectivity, linearity (coefficient of correlation > 0.99), precision (relative standard deviation below 5%), and accuracy (recovery > 96).http://dx.doi.org/10.1155/2017/6969358 |
| spellingShingle | Paula Karina S. Uchoa Leandro Bezerra de Lima Antonia T. A. Pimenta Maria da Conceição F. de Oliveira Jair Mafezoli Mary Anne S. Lima HPLC Quantification of Cytotoxic Compounds from Aspergillus niger Journal of Chemistry |
| title | HPLC Quantification of Cytotoxic Compounds from Aspergillus niger |
| title_full | HPLC Quantification of Cytotoxic Compounds from Aspergillus niger |
| title_fullStr | HPLC Quantification of Cytotoxic Compounds from Aspergillus niger |
| title_full_unstemmed | HPLC Quantification of Cytotoxic Compounds from Aspergillus niger |
| title_short | HPLC Quantification of Cytotoxic Compounds from Aspergillus niger |
| title_sort | hplc quantification of cytotoxic compounds from aspergillus niger |
| url | http://dx.doi.org/10.1155/2017/6969358 |
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