Development and application of sugarcane yellow leaf virus vectors in sugarcane
Abstract The aphid-transmitted polerovirus, sugarcane yellow leaf virus (SCYLV), is one of the most widespread sugarcane viruses and causes huge economic losses to the global sugarcane production. Molecular studies and disease resistance breeding against SCYLV are impeded both by the lack of infecti...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-01-01
|
| Series: | Phytopathology Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s42483-024-00295-0 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Abstract The aphid-transmitted polerovirus, sugarcane yellow leaf virus (SCYLV), is one of the most widespread sugarcane viruses and causes huge economic losses to the global sugarcane production. Molecular studies and disease resistance breeding against SCYLV are impeded both by the lack of infectious clones and comprehensive knowledge of this virus. In this study, we present the development of the first infectious cDNA clone of SCYLV, which could efficiently infect Nicotiana benthamiana, Arabidopsis thaliana and sugarcane plants. SCYLV-based recombinant heterologous EGFP protein vector was also developed. Using this system, we identified the green peach aphid, Myzus persicae, as a new compatible transmission vector and recognized LC05-136 and GT10-2118 as more resistant cultivars of SCYLV than Badila. This system provides an indispensable resource for both SCYLV biological research and sugarcane breeding efforts. |
|---|---|
| ISSN: | 2524-4167 |