Epidemiological Analysis of a <i>K. pneumoniae</i> NDM Outbreak in a Temporary Ward for Patients with Primary COVID-19 Infection
The COVID-19 pandemic made the medical community realize how large a problem it would face. The epidemiological situation forced the opening of additional wards, the so-called “COVID wards”, where an increase in the rate of coexisting bacterial infections was observed. We report a hospital outbreak...
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2025-01-01
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| author | Agata Pruss Klaudia Miładowska Helena Masiuk Paweł Kwiatkowski Joanna Jursa-Kulesza Bartosz Wojciuk Stefania Giedrys-Kalemba Barbara Dołęgowska |
| author_facet | Agata Pruss Klaudia Miładowska Helena Masiuk Paweł Kwiatkowski Joanna Jursa-Kulesza Bartosz Wojciuk Stefania Giedrys-Kalemba Barbara Dołęgowska |
| author_sort | Agata Pruss |
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| description | The COVID-19 pandemic made the medical community realize how large a problem it would face. The epidemiological situation forced the opening of additional wards, the so-called “COVID wards”, where an increase in the rate of coexisting bacterial infections was observed. We report a hospital outbreak due to New Delhi carbapenemases producing <i>K. pneumoniae</i> clones. Twenty-eight <i>K. pneumoniae</i> strains were analyzed from patients with primary COVID-19 infection. The drug susceptibility of the strains was determined by the diffusion–circulation method and E-test. Phenotypic and PCR methods confirmed the production of carbapenemases. The phylogenetic similarity of the obtained strains was examined using pulsed-field electrophoresis. Most strains were isolated from bronchoalveolar lavage. All isolates obtained were resistant to β-lactams and fluoroquinolones. All strains produced New Delhi carbapenemases and were classified into two genetic clusters, A and B. Eight risk factors for secondary bacterial infection were analyzed. Following an intervention involving hand hygiene, strict contact prevention, and cleaning of the hospital environment and medical devices, this outbreak was successfully brought under control. |
| format | Article |
| id | doaj-art-3ae0b4b5f06d48d8a2679ef03c9cca42 |
| institution | Kabale University |
| issn | 2036-7481 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Microbiology Research |
| spelling | doaj-art-3ae0b4b5f06d48d8a2679ef03c9cca422025-01-24T13:41:45ZengMDPI AGMicrobiology Research2036-74812025-01-011611710.3390/microbiolres16010017Epidemiological Analysis of a <i>K. pneumoniae</i> NDM Outbreak in a Temporary Ward for Patients with Primary COVID-19 InfectionAgata Pruss0Klaudia Miładowska1Helena Masiuk2Paweł Kwiatkowski3Joanna Jursa-Kulesza4Bartosz Wojciuk5Stefania Giedrys-Kalemba6Barbara Dołęgowska7Department of Laboratory Medicine, Pomeranian Medical University in Szczecin, Powstancow Wielkopolskich 72, 70-111 Szczecin, PolandDepartment of Laboratory Medicine, Pomeranian Medical University in Szczecin, Powstancow Wielkopolskich 72, 70-111 Szczecin, PolandDepartment of Medical Microbiology, Pomeranian Medical University in Szczecin, Powstancow Wielkopolskich 72, 70-111 Szczecin, PolandDepartment of Diagnostic Immunology, Pomeranian Medical University in Szczecin, Powstancow Wielkopolskich 72, 70-111 Szczecin, PolandDepartment of Medical Microbiology, Pomeranian Medical University in Szczecin, Powstancow Wielkopolskich 72, 70-111 Szczecin, PolandDepartment of Diagnostic Immunology, Pomeranian Medical University in Szczecin, Powstancow Wielkopolskich 72, 70-111 Szczecin, PolandDepartment of Laboratory Medicine, Pomeranian Medical University in Szczecin, Powstancow Wielkopolskich 72, 70-111 Szczecin, PolandDepartment of Laboratory Medicine, Pomeranian Medical University in Szczecin, Powstancow Wielkopolskich 72, 70-111 Szczecin, PolandThe COVID-19 pandemic made the medical community realize how large a problem it would face. The epidemiological situation forced the opening of additional wards, the so-called “COVID wards”, where an increase in the rate of coexisting bacterial infections was observed. We report a hospital outbreak due to New Delhi carbapenemases producing <i>K. pneumoniae</i> clones. Twenty-eight <i>K. pneumoniae</i> strains were analyzed from patients with primary COVID-19 infection. The drug susceptibility of the strains was determined by the diffusion–circulation method and E-test. Phenotypic and PCR methods confirmed the production of carbapenemases. The phylogenetic similarity of the obtained strains was examined using pulsed-field electrophoresis. Most strains were isolated from bronchoalveolar lavage. All isolates obtained were resistant to β-lactams and fluoroquinolones. All strains produced New Delhi carbapenemases and were classified into two genetic clusters, A and B. Eight risk factors for secondary bacterial infection were analyzed. Following an intervention involving hand hygiene, strict contact prevention, and cleaning of the hospital environment and medical devices, this outbreak was successfully brought under control.https://www.mdpi.com/2036-7481/16/1/17<i>Klebsiella pneumoniae</i>SARS-CoV-2New Delhi carbapenemasesoutbreak |
| spellingShingle | Agata Pruss Klaudia Miładowska Helena Masiuk Paweł Kwiatkowski Joanna Jursa-Kulesza Bartosz Wojciuk Stefania Giedrys-Kalemba Barbara Dołęgowska Epidemiological Analysis of a <i>K. pneumoniae</i> NDM Outbreak in a Temporary Ward for Patients with Primary COVID-19 Infection Microbiology Research <i>Klebsiella pneumoniae</i> SARS-CoV-2 New Delhi carbapenemases outbreak |
| title | Epidemiological Analysis of a <i>K. pneumoniae</i> NDM Outbreak in a Temporary Ward for Patients with Primary COVID-19 Infection |
| title_full | Epidemiological Analysis of a <i>K. pneumoniae</i> NDM Outbreak in a Temporary Ward for Patients with Primary COVID-19 Infection |
| title_fullStr | Epidemiological Analysis of a <i>K. pneumoniae</i> NDM Outbreak in a Temporary Ward for Patients with Primary COVID-19 Infection |
| title_full_unstemmed | Epidemiological Analysis of a <i>K. pneumoniae</i> NDM Outbreak in a Temporary Ward for Patients with Primary COVID-19 Infection |
| title_short | Epidemiological Analysis of a <i>K. pneumoniae</i> NDM Outbreak in a Temporary Ward for Patients with Primary COVID-19 Infection |
| title_sort | epidemiological analysis of a i k pneumoniae i ndm outbreak in a temporary ward for patients with primary covid 19 infection |
| topic | <i>Klebsiella pneumoniae</i> SARS-CoV-2 New Delhi carbapenemases outbreak |
| url | https://www.mdpi.com/2036-7481/16/1/17 |
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