Overexpression of PLCG2 and TMEM38A inhibit tumor progression in clear cell renal cell carcinoma
Abstract Clear cell renal cell carcinoma is a prevalent urological malignancy, imposing substantial burdens on both patients and society. In our study, we used bioinformatics methods to select four putative target genes associated with EMT and prognosis and developed a nomogram model which could acc...
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-86644-1 |
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| author | Yiqiao Zhao Liang Yang Xiaojie Bai Lu Du Huan Lai Yiyang Liu Ping Chen Michael E. DiSanto Xinhua Zhang |
| author_facet | Yiqiao Zhao Liang Yang Xiaojie Bai Lu Du Huan Lai Yiyang Liu Ping Chen Michael E. DiSanto Xinhua Zhang |
| author_sort | Yiqiao Zhao |
| collection | DOAJ |
| description | Abstract Clear cell renal cell carcinoma is a prevalent urological malignancy, imposing substantial burdens on both patients and society. In our study, we used bioinformatics methods to select four putative target genes associated with EMT and prognosis and developed a nomogram model which could accurately predicting 5-year patient survival rates. We further analyzed proteome and single-cell data and selected PLCG2 and TMEM38A for the following experiments. Overexpression models of PLCG2 and TMEM38A were generated in Caki-1 and 786-O cell lines using plasmids. The in vitro experiments demonstrated that both of them exerted pro-apoptotic effects on Caki-1 and 786-O cells, inducing G2/M phase arrest, inhibiting proliferation, and suppressing EMT. In summary, we identified potential tumor suppressor factors and stratified ccRCC patients into high-risk and low-risk groups based on these factors. Furthermore, we elucidated the impact of PLCG2 and TMEM38A in Caki-1 and 786-O cell lines, offering novel avenues for therapeutic target exploration. |
| format | Article |
| id | doaj-art-3adbe844536645b89dbae5fa18750abf |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-3adbe844536645b89dbae5fa18750abf2025-01-26T12:24:49ZengNature PortfolioScientific Reports2045-23222025-01-0115111410.1038/s41598-025-86644-1Overexpression of PLCG2 and TMEM38A inhibit tumor progression in clear cell renal cell carcinomaYiqiao Zhao0Liang Yang1Xiaojie Bai2Lu Du3Huan Lai4Yiyang Liu5Ping Chen6Michael E. DiSanto7Xinhua Zhang8Department of Urology, Zhongnan Hospital of Wuhan UniversityDepartment of Urology, Zhongnan Hospital of Wuhan UniversityDepartment of Urology, Zhongnan Hospital of Wuhan UniversityDepartment of Urology, Zhongnan Hospital of Wuhan UniversityDepartment of Urology, Zhongnan Hospital of Wuhan UniversityDepartment of Urology, Zhongnan Hospital of Wuhan UniversityDepartment of Urology, Zhongnan Hospital of Wuhan UniversityDepartment of Surgery and Biomedical Sciences, Cooper Medical School of Rowan UniversityDepartment of Urology, Zhongnan Hospital of Wuhan UniversityAbstract Clear cell renal cell carcinoma is a prevalent urological malignancy, imposing substantial burdens on both patients and society. In our study, we used bioinformatics methods to select four putative target genes associated with EMT and prognosis and developed a nomogram model which could accurately predicting 5-year patient survival rates. We further analyzed proteome and single-cell data and selected PLCG2 and TMEM38A for the following experiments. Overexpression models of PLCG2 and TMEM38A were generated in Caki-1 and 786-O cell lines using plasmids. The in vitro experiments demonstrated that both of them exerted pro-apoptotic effects on Caki-1 and 786-O cells, inducing G2/M phase arrest, inhibiting proliferation, and suppressing EMT. In summary, we identified potential tumor suppressor factors and stratified ccRCC patients into high-risk and low-risk groups based on these factors. Furthermore, we elucidated the impact of PLCG2 and TMEM38A in Caki-1 and 786-O cell lines, offering novel avenues for therapeutic target exploration.https://doi.org/10.1038/s41598-025-86644-1ccRCCPLCG2TMEM38ATCGAPDC |
| spellingShingle | Yiqiao Zhao Liang Yang Xiaojie Bai Lu Du Huan Lai Yiyang Liu Ping Chen Michael E. DiSanto Xinhua Zhang Overexpression of PLCG2 and TMEM38A inhibit tumor progression in clear cell renal cell carcinoma Scientific Reports ccRCC PLCG2 TMEM38A TCGA PDC |
| title | Overexpression of PLCG2 and TMEM38A inhibit tumor progression in clear cell renal cell carcinoma |
| title_full | Overexpression of PLCG2 and TMEM38A inhibit tumor progression in clear cell renal cell carcinoma |
| title_fullStr | Overexpression of PLCG2 and TMEM38A inhibit tumor progression in clear cell renal cell carcinoma |
| title_full_unstemmed | Overexpression of PLCG2 and TMEM38A inhibit tumor progression in clear cell renal cell carcinoma |
| title_short | Overexpression of PLCG2 and TMEM38A inhibit tumor progression in clear cell renal cell carcinoma |
| title_sort | overexpression of plcg2 and tmem38a inhibit tumor progression in clear cell renal cell carcinoma |
| topic | ccRCC PLCG2 TMEM38A TCGA PDC |
| url | https://doi.org/10.1038/s41598-025-86644-1 |
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