A Refined Nomenclature System to Better Discriminate Endo- and Exo-Type Fructanases and Glucanases
Distinguishing between endo- and exo-type enzymes within the glycoside hydrolase (GH) classification presents significant challenges. Traditional methods, often based on endpoint activity measurements, do not capture the full range of products generated, leading to inconsistencies in classification....
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
|
| Series: | Biomolecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-273X/15/1/11 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832588963821387776 |
|---|---|
| author | Laura Leaerts Jaime Ricardo Porras-Domínguez Maxime Versluys Wim Van den Ende |
| author_facet | Laura Leaerts Jaime Ricardo Porras-Domínguez Maxime Versluys Wim Van den Ende |
| author_sort | Laura Leaerts |
| collection | DOAJ |
| description | Distinguishing between endo- and exo-type enzymes within the glycoside hydrolase (GH) classification presents significant challenges. Traditional methods, often based on endpoint activity measurements, do not capture the full range of products generated, leading to inconsistencies in classification. Not all exo-acting fructanases and glucanases produce monosaccharides (like fructose or glucose), while endo-acting enzymes do not solely produce higher-degree polymerization oligosaccharides. In practice, both enzyme types can yield a variety of products throughout the reaction, complicating classification efforts. To address these challenges, we propose a refined nomenclature system for GH enzymes, including fructanases and glucanases, based on good practices and initial product formation. This system classifies enzymes into four categories for each type: Fr, Fn, Fn,n+1 and F1 for fructanases, and Gr, Gn, Gn,n+1 and G1 for glucanases, based on their mode of action (endo- or exo-based) and initial product profiles. Our refined nomenclature system will advance enzyme structure–function research and support the production and application of fructan and glucan oligosaccharides as prebiotics, priming agents, and potential signaling molecules in microbe–microbe and plant–microbe interactions. Ultimately, this system could benefit agronomy and the food industry, contributing to health improvements. |
| format | Article |
| id | doaj-art-3ad5e52443c1430bb5b31c3debd7c059 |
| institution | Kabale University |
| issn | 2218-273X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj-art-3ad5e52443c1430bb5b31c3debd7c0592025-01-24T13:24:51ZengMDPI AGBiomolecules2218-273X2024-12-011511110.3390/biom15010011A Refined Nomenclature System to Better Discriminate Endo- and Exo-Type Fructanases and GlucanasesLaura Leaerts0Jaime Ricardo Porras-Domínguez1Maxime Versluys2Wim Van den Ende3Laboratory of Molecular Plant Biology and KU Leuven Plant Institute, Kasteelpark Arenberg 31, 3001 Leuven, BelgiumLaboratory of Molecular Plant Biology and KU Leuven Plant Institute, Kasteelpark Arenberg 31, 3001 Leuven, BelgiumLaboratory of Molecular Plant Biology and KU Leuven Plant Institute, Kasteelpark Arenberg 31, 3001 Leuven, BelgiumLaboratory of Molecular Plant Biology and KU Leuven Plant Institute, Kasteelpark Arenberg 31, 3001 Leuven, BelgiumDistinguishing between endo- and exo-type enzymes within the glycoside hydrolase (GH) classification presents significant challenges. Traditional methods, often based on endpoint activity measurements, do not capture the full range of products generated, leading to inconsistencies in classification. Not all exo-acting fructanases and glucanases produce monosaccharides (like fructose or glucose), while endo-acting enzymes do not solely produce higher-degree polymerization oligosaccharides. In practice, both enzyme types can yield a variety of products throughout the reaction, complicating classification efforts. To address these challenges, we propose a refined nomenclature system for GH enzymes, including fructanases and glucanases, based on good practices and initial product formation. This system classifies enzymes into four categories for each type: Fr, Fn, Fn,n+1 and F1 for fructanases, and Gr, Gn, Gn,n+1 and G1 for glucanases, based on their mode of action (endo- or exo-based) and initial product profiles. Our refined nomenclature system will advance enzyme structure–function research and support the production and application of fructan and glucan oligosaccharides as prebiotics, priming agents, and potential signaling molecules in microbe–microbe and plant–microbe interactions. Ultimately, this system could benefit agronomy and the food industry, contributing to health improvements.https://www.mdpi.com/2218-273X/15/1/11endo/exo mechanismfructanasesglucanasesGH32 familyinulinlevan |
| spellingShingle | Laura Leaerts Jaime Ricardo Porras-Domínguez Maxime Versluys Wim Van den Ende A Refined Nomenclature System to Better Discriminate Endo- and Exo-Type Fructanases and Glucanases Biomolecules endo/exo mechanism fructanases glucanases GH32 family inulin levan |
| title | A Refined Nomenclature System to Better Discriminate Endo- and Exo-Type Fructanases and Glucanases |
| title_full | A Refined Nomenclature System to Better Discriminate Endo- and Exo-Type Fructanases and Glucanases |
| title_fullStr | A Refined Nomenclature System to Better Discriminate Endo- and Exo-Type Fructanases and Glucanases |
| title_full_unstemmed | A Refined Nomenclature System to Better Discriminate Endo- and Exo-Type Fructanases and Glucanases |
| title_short | A Refined Nomenclature System to Better Discriminate Endo- and Exo-Type Fructanases and Glucanases |
| title_sort | refined nomenclature system to better discriminate endo and exo type fructanases and glucanases |
| topic | endo/exo mechanism fructanases glucanases GH32 family inulin levan |
| url | https://www.mdpi.com/2218-273X/15/1/11 |
| work_keys_str_mv | AT lauraleaerts arefinednomenclaturesystemtobetterdiscriminateendoandexotypefructanasesandglucanases AT jaimericardoporrasdominguez arefinednomenclaturesystemtobetterdiscriminateendoandexotypefructanasesandglucanases AT maximeversluys arefinednomenclaturesystemtobetterdiscriminateendoandexotypefructanasesandglucanases AT wimvandenende arefinednomenclaturesystemtobetterdiscriminateendoandexotypefructanasesandglucanases AT lauraleaerts refinednomenclaturesystemtobetterdiscriminateendoandexotypefructanasesandglucanases AT jaimericardoporrasdominguez refinednomenclaturesystemtobetterdiscriminateendoandexotypefructanasesandglucanases AT maximeversluys refinednomenclaturesystemtobetterdiscriminateendoandexotypefructanasesandglucanases AT wimvandenende refinednomenclaturesystemtobetterdiscriminateendoandexotypefructanasesandglucanases |