Senyawa Fucodiphlorethol Dan Phloroglucinol Alga Coklat Sebagai Inhibitor Lipase Secara In Silico
Brown algae is a marine functional food that high fibers, vitamins, and bioactive compounds. Brown algae improved blood glucose and lipid profile. This study investigated the potential activity of two bioactive compounds from brown algae, as anti-obesity through pancreatic lipase inhibition. Molecul...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | Indonesian |
| Published: |
Universitas PGRI Madiun
2022-05-01
|
| Series: | Florea: Jurnal Biologi dan Pembelajarannya |
| Subjects: | |
| Online Access: | https://e-journal.unipma.ac.id/index.php/JF/article/view/11743 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832594063097856000 |
|---|---|
| author | Yohanes Bare Dewi Ratih Tirto Sari Marsiana Coo Mogi Maria Marcelina Dua Nurak |
| author_facet | Yohanes Bare Dewi Ratih Tirto Sari Marsiana Coo Mogi Maria Marcelina Dua Nurak |
| author_sort | Yohanes Bare |
| collection | DOAJ |
| description | Brown algae is a marine functional food that high fibers, vitamins, and bioactive compounds. Brown algae improved blood glucose and lipid profile. This study investigated the potential activity of two bioactive compounds from brown algae, as anti-obesity through pancreatic lipase inhibition. Molecular docking was conducted for investigating the lipase inhibition of bioactive compounds. Lipase structure (PDB ID 1ETH) was obtained from protein data bank and docked with fucodiphlorethol and phloroglucinol in specific position. Then, the complex was analyzed by PyMol following by Discovery Studio version 21.1.1. Phloroglucinol - pancreatic lipase showed five amino acid residues by hydrogen bonds, hydrophobic and electrostatic. Â Fucodiphlorethol - pancreatic lipase generated binding energy -392 kJ/mol and consisted of eight hydrogen bonds, three electrostatic, and 4 hydrophobic interactions. The active sites of fucodiphlorethol and phloroglucinol with lipase were not considered as active sites or catalytic sites. Therefore, we predicted that both of fucodiphlorethol and phloroglucinol inhibit lipase allosterically by altering the protein structure conformations. molecular docking analysis suggested that fucodiphlorethol and phloroglucinol have potential antiobesity effect by inhibiting lipase. Molecular dynamic are needed for further investigation. |
| format | Article |
| id | doaj-art-3a89bc3f2e10465482852a28323a06b0 |
| institution | Kabale University |
| issn | 2355-6102 2502-0404 |
| language | Indonesian |
| publishDate | 2022-05-01 |
| publisher | Universitas PGRI Madiun |
| record_format | Article |
| series | Florea: Jurnal Biologi dan Pembelajarannya |
| spelling | doaj-art-3a89bc3f2e10465482852a28323a06b02025-01-20T06:29:35ZindUniversitas PGRI MadiunFlorea: Jurnal Biologi dan Pembelajarannya2355-61022502-04042022-05-0191535910.25273/florea.v9i1.117438339Senyawa Fucodiphlorethol Dan Phloroglucinol Alga Coklat Sebagai Inhibitor Lipase Secara In SilicoYohanes Bare0Dewi Ratih Tirto Sari1Marsiana Coo Mogi2Maria Marcelina Dua Nurak3Universitas Nusa Nipa IndonesiaUniversitas IbrahimyUniversitas Nusa Nipa IndonesiaUniversitas Nusa Nipa IndonesiaBrown algae is a marine functional food that high fibers, vitamins, and bioactive compounds. Brown algae improved blood glucose and lipid profile. This study investigated the potential activity of two bioactive compounds from brown algae, as anti-obesity through pancreatic lipase inhibition. Molecular docking was conducted for investigating the lipase inhibition of bioactive compounds. Lipase structure (PDB ID 1ETH) was obtained from protein data bank and docked with fucodiphlorethol and phloroglucinol in specific position. Then, the complex was analyzed by PyMol following by Discovery Studio version 21.1.1. Phloroglucinol - pancreatic lipase showed five amino acid residues by hydrogen bonds, hydrophobic and electrostatic. Â Fucodiphlorethol - pancreatic lipase generated binding energy -392 kJ/mol and consisted of eight hydrogen bonds, three electrostatic, and 4 hydrophobic interactions. The active sites of fucodiphlorethol and phloroglucinol with lipase were not considered as active sites or catalytic sites. Therefore, we predicted that both of fucodiphlorethol and phloroglucinol inhibit lipase allosterically by altering the protein structure conformations. molecular docking analysis suggested that fucodiphlorethol and phloroglucinol have potential antiobesity effect by inhibiting lipase. Molecular dynamic are needed for further investigation.https://e-journal.unipma.ac.id/index.php/JF/article/view/11743antiobesityfucodiphloretholin silicolipasephloroglucinol |
| spellingShingle | Yohanes Bare Dewi Ratih Tirto Sari Marsiana Coo Mogi Maria Marcelina Dua Nurak Senyawa Fucodiphlorethol Dan Phloroglucinol Alga Coklat Sebagai Inhibitor Lipase Secara In Silico Florea: Jurnal Biologi dan Pembelajarannya antiobesity fucodiphlorethol in silico lipase phloroglucinol |
| title | Senyawa Fucodiphlorethol Dan Phloroglucinol Alga Coklat Sebagai Inhibitor Lipase Secara In Silico |
| title_full | Senyawa Fucodiphlorethol Dan Phloroglucinol Alga Coklat Sebagai Inhibitor Lipase Secara In Silico |
| title_fullStr | Senyawa Fucodiphlorethol Dan Phloroglucinol Alga Coklat Sebagai Inhibitor Lipase Secara In Silico |
| title_full_unstemmed | Senyawa Fucodiphlorethol Dan Phloroglucinol Alga Coklat Sebagai Inhibitor Lipase Secara In Silico |
| title_short | Senyawa Fucodiphlorethol Dan Phloroglucinol Alga Coklat Sebagai Inhibitor Lipase Secara In Silico |
| title_sort | senyawa fucodiphlorethol dan phloroglucinol alga coklat sebagai inhibitor lipase secara in silico |
| topic | antiobesity fucodiphlorethol in silico lipase phloroglucinol |
| url | https://e-journal.unipma.ac.id/index.php/JF/article/view/11743 |
| work_keys_str_mv | AT yohanesbare senyawafucodiphloretholdanphloroglucinolalgacoklatsebagaiinhibitorlipasesecarainsilico AT dewiratihtirtosari senyawafucodiphloretholdanphloroglucinolalgacoklatsebagaiinhibitorlipasesecarainsilico AT marsianacoomogi senyawafucodiphloretholdanphloroglucinolalgacoklatsebagaiinhibitorlipasesecarainsilico AT mariamarcelinaduanurak senyawafucodiphloretholdanphloroglucinolalgacoklatsebagaiinhibitorlipasesecarainsilico |