Diallyl Disulfide Suppresses Inflammatory and Oxidative Machineries following Carrageenan Injection-Induced Paw Edema in Mice
Diallyl disulfide (DADS) is the major organosulfur constituent in garlic, with a variety of pharmacological activities including antioxidant and anti-inflammatory. Here, we examined the potential antiedematous impact of DADS- versus carrageenan-mediated paw edema in mice. Carrageenan injection poten...
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| Format: | Article |
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Wiley
2020-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2020/8508906 |
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| author | Hongxing Zhang Chi Shang Zhao Tian Hatem K. Amin Rami B. Kassab Ahmed E. Abdel Moneim Yingang Zhang |
| author_facet | Hongxing Zhang Chi Shang Zhao Tian Hatem K. Amin Rami B. Kassab Ahmed E. Abdel Moneim Yingang Zhang |
| author_sort | Hongxing Zhang |
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| description | Diallyl disulfide (DADS) is the major organosulfur constituent in garlic, with a variety of pharmacological activities including antioxidant and anti-inflammatory. Here, we examined the potential antiedematous impact of DADS- versus carrageenan-mediated paw edema in mice. Carrageenan injection potentiated an inflammatory reaction as presented by the elevated serological C-reactive protein (CRP) levels and transcription of tumor necrosis factor-alpha (TNF-α, Tnfα), interleukin-1 beta (IL-1β, Il1b), interleukin-2 (IL-2, Il2), inducible nitric oxide synthase (iNOS), nitric oxide (NO), cyclooxygenase-2 (COX-2, Ptgs2), prostaglandin E2 (PGE2), monocyte chemoattractant protein-1 (MCP-1, Ccl1), nuclear factor kappa B (NF-κB), and myeloperoxidase (MPO) activity, while interleukin-10 (IL-10) was declined in the injured paw tissue. Additionally, carrageenan elevated lipid peroxidation in terms of malondialdehyde (MDA) and decreased glutathione content (GSH). Remarkably, DADS was found to inhibit the inflammatory signaling, suppressed the developed oxidative damage, and protected the histopathological alterations in the inflamed paw tissue in response to carrageenan injection. Our findings suggest that DADS could be used as an alternative therapy used to alleviate the pathophysiological changes associated with the genesis of paw edema through its potent anti-inflammatory and antioxidant impacts. |
| format | Article |
| id | doaj-art-3a3eb79b2d0f4487926faf09d9265de9 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
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| series | Mediators of Inflammation |
| spelling | doaj-art-3a3eb79b2d0f4487926faf09d9265de92025-02-03T06:05:18ZengWileyMediators of Inflammation0962-93511466-18612020-01-01202010.1155/2020/85089068508906Diallyl Disulfide Suppresses Inflammatory and Oxidative Machineries following Carrageenan Injection-Induced Paw Edema in MiceHongxing Zhang0Chi Shang1Zhao Tian2Hatem K. Amin3Rami B. Kassab4Ahmed E. Abdel Moneim5Yingang Zhang6Department of Orthopaedic Surgery, The First Affiliated Hospital of Xi’an Jiaotong University, No. 277 West Yanta Road, Yanta District, Xi’an, Shaanxi 710061, ChinaDepartment of Hand Surgery, Xi’an Honghui Hospital, No. 555 Youyi East Road, Beilin District, Xi’an, Shaanxi 710054, ChinaDepartment of Hand Surgery, Xi’an Honghui Hospital, No. 555 Youyi East Road, Beilin District, Xi’an, Shaanxi 710054, ChinaDepartment of Biochemistry and Molecular Biology, Faculty of Pharmacy, Helwan University, Cairo 11795, EgyptZoology and Entomology Department, Faculty of Science, Helwan University, Cairo 11795, EgyptZoology and Entomology Department, Faculty of Science, Helwan University, Cairo 11795, EgyptDepartment of Orthopaedic Surgery, The First Affiliated Hospital of Xi’an Jiaotong University, No. 277 West Yanta Road, Yanta District, Xi’an, Shaanxi 710061, ChinaDiallyl disulfide (DADS) is the major organosulfur constituent in garlic, with a variety of pharmacological activities including antioxidant and anti-inflammatory. Here, we examined the potential antiedematous impact of DADS- versus carrageenan-mediated paw edema in mice. Carrageenan injection potentiated an inflammatory reaction as presented by the elevated serological C-reactive protein (CRP) levels and transcription of tumor necrosis factor-alpha (TNF-α, Tnfα), interleukin-1 beta (IL-1β, Il1b), interleukin-2 (IL-2, Il2), inducible nitric oxide synthase (iNOS), nitric oxide (NO), cyclooxygenase-2 (COX-2, Ptgs2), prostaglandin E2 (PGE2), monocyte chemoattractant protein-1 (MCP-1, Ccl1), nuclear factor kappa B (NF-κB), and myeloperoxidase (MPO) activity, while interleukin-10 (IL-10) was declined in the injured paw tissue. Additionally, carrageenan elevated lipid peroxidation in terms of malondialdehyde (MDA) and decreased glutathione content (GSH). Remarkably, DADS was found to inhibit the inflammatory signaling, suppressed the developed oxidative damage, and protected the histopathological alterations in the inflamed paw tissue in response to carrageenan injection. Our findings suggest that DADS could be used as an alternative therapy used to alleviate the pathophysiological changes associated with the genesis of paw edema through its potent anti-inflammatory and antioxidant impacts.http://dx.doi.org/10.1155/2020/8508906 |
| spellingShingle | Hongxing Zhang Chi Shang Zhao Tian Hatem K. Amin Rami B. Kassab Ahmed E. Abdel Moneim Yingang Zhang Diallyl Disulfide Suppresses Inflammatory and Oxidative Machineries following Carrageenan Injection-Induced Paw Edema in Mice Mediators of Inflammation |
| title | Diallyl Disulfide Suppresses Inflammatory and Oxidative Machineries following Carrageenan Injection-Induced Paw Edema in Mice |
| title_full | Diallyl Disulfide Suppresses Inflammatory and Oxidative Machineries following Carrageenan Injection-Induced Paw Edema in Mice |
| title_fullStr | Diallyl Disulfide Suppresses Inflammatory and Oxidative Machineries following Carrageenan Injection-Induced Paw Edema in Mice |
| title_full_unstemmed | Diallyl Disulfide Suppresses Inflammatory and Oxidative Machineries following Carrageenan Injection-Induced Paw Edema in Mice |
| title_short | Diallyl Disulfide Suppresses Inflammatory and Oxidative Machineries following Carrageenan Injection-Induced Paw Edema in Mice |
| title_sort | diallyl disulfide suppresses inflammatory and oxidative machineries following carrageenan injection induced paw edema in mice |
| url | http://dx.doi.org/10.1155/2020/8508906 |
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