Carbon Dioxide Micro-Nano Bubbles Aeration Improves Carbon Fixation Efficiency for Succinic Acid Synthesis by <i>Escherichia coli</i>
The low solubility of CO<sub>2</sub> in water leads to massive CO<sub>2</sub> emission and extremely low CO<sub>2</sub> utilization in succinic acid (SA) biosynthesis. To enhance microbial CO<sub>2</sub> utilization, micro-nano bubbles (MNBs) were indu...
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2025-01-01
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| Series: | Fermentation |
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| author | Ying Chen Hao Wu Qianqian Huang Jingwen Liao Liuqing Wang Yue Pan Anming Xu Wenming Zhang Min Jiang |
| author_facet | Ying Chen Hao Wu Qianqian Huang Jingwen Liao Liuqing Wang Yue Pan Anming Xu Wenming Zhang Min Jiang |
| author_sort | Ying Chen |
| collection | DOAJ |
| description | The low solubility of CO<sub>2</sub> in water leads to massive CO<sub>2</sub> emission and extremely low CO<sub>2</sub> utilization in succinic acid (SA) biosynthesis. To enhance microbial CO<sub>2</sub> utilization, micro-nano bubbles (MNBs) were induced in SA biosynthesis by <i>E. coli</i> Suc260 in this study. The results showed that MNB aeration decreased CO<sub>2</sub> emissions and increased CO<sub>2</sub> solubility in the medium significantly. The CO<sub>2</sub> utilization of MNB aeration was 129.69% higher than that of bubble aeration in atmospheric fermentation. However, MNBs showed a significant inhibitory effect on bacterial growth in the pressurized environment, although a two-stage aerobic–anaerobic fermentation strategy weakened the inhibition. The biofilm-enhanced strain <i>E. coli</i> Suc260-CsgA showed a strong tolerance to MNBs. In pressurized fermentation with MNB aeration, the actual CO<sub>2</sub> utilization of <i>E. coli</i> Suc260-CsgA was 30.63% at 0.18 MPa, which was a 6.49-times improvement. The CO<sub>2</sub> requirement for SA synthesis decreased by 83.4%, and the fugitive emission of CO<sub>2</sub> was successfully controlled. The activities of key enzymes within the SA synthesis pathway were also maintained or enhanced in the fermentation process with MNB aeration. These results indicated that the biofilm-enhanced strain and CO<sub>2</sub>-MNBs could improve carbon fixation efficiency in microbial carbon sequestration. |
| format | Article |
| id | doaj-art-3a1bcf61dda44cd7a82dfca8c7c58cba |
| institution | Kabale University |
| issn | 2311-5637 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj-art-3a1bcf61dda44cd7a82dfca8c7c58cba2025-01-24T13:32:07ZengMDPI AGFermentation2311-56372025-01-011113110.3390/fermentation11010031Carbon Dioxide Micro-Nano Bubbles Aeration Improves Carbon Fixation Efficiency for Succinic Acid Synthesis by <i>Escherichia coli</i>Ying Chen0Hao Wu1Qianqian Huang2Jingwen Liao3Liuqing Wang4Yue Pan5Anming Xu6Wenming Zhang7Min Jiang8College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, ChinaCollege of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, ChinaCollege of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, ChinaCollege of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, ChinaCollege of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, ChinaCollege of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, ChinaCollege of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, ChinaCollege of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, ChinaCollege of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, ChinaThe low solubility of CO<sub>2</sub> in water leads to massive CO<sub>2</sub> emission and extremely low CO<sub>2</sub> utilization in succinic acid (SA) biosynthesis. To enhance microbial CO<sub>2</sub> utilization, micro-nano bubbles (MNBs) were induced in SA biosynthesis by <i>E. coli</i> Suc260 in this study. The results showed that MNB aeration decreased CO<sub>2</sub> emissions and increased CO<sub>2</sub> solubility in the medium significantly. The CO<sub>2</sub> utilization of MNB aeration was 129.69% higher than that of bubble aeration in atmospheric fermentation. However, MNBs showed a significant inhibitory effect on bacterial growth in the pressurized environment, although a two-stage aerobic–anaerobic fermentation strategy weakened the inhibition. The biofilm-enhanced strain <i>E. coli</i> Suc260-CsgA showed a strong tolerance to MNBs. In pressurized fermentation with MNB aeration, the actual CO<sub>2</sub> utilization of <i>E. coli</i> Suc260-CsgA was 30.63% at 0.18 MPa, which was a 6.49-times improvement. The CO<sub>2</sub> requirement for SA synthesis decreased by 83.4%, and the fugitive emission of CO<sub>2</sub> was successfully controlled. The activities of key enzymes within the SA synthesis pathway were also maintained or enhanced in the fermentation process with MNB aeration. These results indicated that the biofilm-enhanced strain and CO<sub>2</sub>-MNBs could improve carbon fixation efficiency in microbial carbon sequestration.https://www.mdpi.com/2311-5637/11/1/31succinic acidmicro-nano bubblesCO<sub>2</sub> utilization<i>Escherichia coli</i>biofilm |
| spellingShingle | Ying Chen Hao Wu Qianqian Huang Jingwen Liao Liuqing Wang Yue Pan Anming Xu Wenming Zhang Min Jiang Carbon Dioxide Micro-Nano Bubbles Aeration Improves Carbon Fixation Efficiency for Succinic Acid Synthesis by <i>Escherichia coli</i> Fermentation succinic acid micro-nano bubbles CO<sub>2</sub> utilization <i>Escherichia coli</i> biofilm |
| title | Carbon Dioxide Micro-Nano Bubbles Aeration Improves Carbon Fixation Efficiency for Succinic Acid Synthesis by <i>Escherichia coli</i> |
| title_full | Carbon Dioxide Micro-Nano Bubbles Aeration Improves Carbon Fixation Efficiency for Succinic Acid Synthesis by <i>Escherichia coli</i> |
| title_fullStr | Carbon Dioxide Micro-Nano Bubbles Aeration Improves Carbon Fixation Efficiency for Succinic Acid Synthesis by <i>Escherichia coli</i> |
| title_full_unstemmed | Carbon Dioxide Micro-Nano Bubbles Aeration Improves Carbon Fixation Efficiency for Succinic Acid Synthesis by <i>Escherichia coli</i> |
| title_short | Carbon Dioxide Micro-Nano Bubbles Aeration Improves Carbon Fixation Efficiency for Succinic Acid Synthesis by <i>Escherichia coli</i> |
| title_sort | carbon dioxide micro nano bubbles aeration improves carbon fixation efficiency for succinic acid synthesis by i escherichia coli i |
| topic | succinic acid micro-nano bubbles CO<sub>2</sub> utilization <i>Escherichia coli</i> biofilm |
| url | https://www.mdpi.com/2311-5637/11/1/31 |
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