Protective Effects of Trehalose on the Corneal Epithelial Cells
Purpose. Aim of the present work was to evaluate the effects of the trehalose on the corneal epithelium undergoing alcohol delamination. Methods. Twelve patients undergoing laser subepithelial keratomileusis (LASEK) were consecutively included in the study. The right eyes were pretreated with 3% tr...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/717835 |
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| author | Pasquale Aragona Pietro Colosi Laura Rania Francesca Colosi Antonina Pisani Domenico Puzzolo Antonio Micali |
| author_facet | Pasquale Aragona Pietro Colosi Laura Rania Francesca Colosi Antonina Pisani Domenico Puzzolo Antonio Micali |
| author_sort | Pasquale Aragona |
| collection | DOAJ |
| description | Purpose. Aim of the present work was to evaluate the effects of the trehalose on the corneal epithelium undergoing alcohol delamination.
Methods. Twelve patients undergoing laser subepithelial keratomileusis (LASEK) were consecutively included in the study. The right eyes were pretreated with 3% trehalose eye drops, whilst left eyes were used as control. Epithelial specimens were processed for cells vitality assessment, apoptosis, and light and transmission electron microscopy; a morphometric analysis was performed in both groups. Results. In both trehalose-untreated eyes (TUE) and trehalose-treated eyes (TTE), the percentage of vital cells was similar and no apoptotic cells were observed. In TUE, the corneal epithelium showed superficial cells with reduced microfolds, wing cells with vesicles and dilated intercellular spaces, and dark basal cells with vesicles and wide clefts. In TTE, superficial and wing cells were better preserved, and basal cells were generally clear with intracytoplasmatic vesicles. The morphometric analysis showed statistically significant differences between the two groups: the TTE epithelial height was higher, the basal cells showed larger area and clearer cytoplasm. The distribution of desmosomes and hemidesmosomes was significantly different between the groups.
Conclusions. Trehalose administration better preserved morphological and morphometric features of alcohol-treated corneal epithelium, when compared to controls. |
| format | Article |
| id | doaj-art-3a03bfa3b4fc462487a3ad1e43114e87 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-3a03bfa3b4fc462487a3ad1e43114e872025-02-03T01:22:19ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/717835717835Protective Effects of Trehalose on the Corneal Epithelial CellsPasquale Aragona0Pietro Colosi1Laura Rania2Francesca Colosi3Antonina Pisani4Domenico Puzzolo5Antonio Micali6Department of Experimental Medical-Surgical Sciences, Regional Referral Center for the Ocular Surface Diseases, University of Messina, Via C. Valeria 1, 98125 Messina, ItalyDepartment of Social and Environmental Health, University of Messina, Via C. Valeria 1, 98125 Messina, ItalyDepartment of Experimental Medical-Surgical Sciences, Regional Referral Center for the Ocular Surface Diseases, University of Messina, Via C. Valeria 1, 98125 Messina, ItalyDepartment of Experimental Medical-Surgical Sciences, Regional Referral Center for the Ocular Surface Diseases, University of Messina, Via C. Valeria 1, 98125 Messina, ItalyDepartment of Biomedical Sciences and Morphofunctional Imaging, Section of Histology and Embryology, University of Messina, Via C. Valeria 1, 98125 Messina, ItalyDepartment of Biomedical Sciences and Morphofunctional Imaging, Section of Histology and Embryology, University of Messina, Via C. Valeria 1, 98125 Messina, ItalyDepartment of Biomedical Sciences and Morphofunctional Imaging, Section of Histology and Embryology, University of Messina, Via C. Valeria 1, 98125 Messina, ItalyPurpose. Aim of the present work was to evaluate the effects of the trehalose on the corneal epithelium undergoing alcohol delamination. Methods. Twelve patients undergoing laser subepithelial keratomileusis (LASEK) were consecutively included in the study. The right eyes were pretreated with 3% trehalose eye drops, whilst left eyes were used as control. Epithelial specimens were processed for cells vitality assessment, apoptosis, and light and transmission electron microscopy; a morphometric analysis was performed in both groups. Results. In both trehalose-untreated eyes (TUE) and trehalose-treated eyes (TTE), the percentage of vital cells was similar and no apoptotic cells were observed. In TUE, the corneal epithelium showed superficial cells with reduced microfolds, wing cells with vesicles and dilated intercellular spaces, and dark basal cells with vesicles and wide clefts. In TTE, superficial and wing cells were better preserved, and basal cells were generally clear with intracytoplasmatic vesicles. The morphometric analysis showed statistically significant differences between the two groups: the TTE epithelial height was higher, the basal cells showed larger area and clearer cytoplasm. The distribution of desmosomes and hemidesmosomes was significantly different between the groups. Conclusions. Trehalose administration better preserved morphological and morphometric features of alcohol-treated corneal epithelium, when compared to controls.http://dx.doi.org/10.1155/2014/717835 |
| spellingShingle | Pasquale Aragona Pietro Colosi Laura Rania Francesca Colosi Antonina Pisani Domenico Puzzolo Antonio Micali Protective Effects of Trehalose on the Corneal Epithelial Cells The Scientific World Journal |
| title | Protective Effects of Trehalose on the Corneal Epithelial Cells |
| title_full | Protective Effects of Trehalose on the Corneal Epithelial Cells |
| title_fullStr | Protective Effects of Trehalose on the Corneal Epithelial Cells |
| title_full_unstemmed | Protective Effects of Trehalose on the Corneal Epithelial Cells |
| title_short | Protective Effects of Trehalose on the Corneal Epithelial Cells |
| title_sort | protective effects of trehalose on the corneal epithelial cells |
| url | http://dx.doi.org/10.1155/2014/717835 |
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