Phenotypic and Functional Changes in Peripheral Blood Natural Killer Cells in Crohn Disease Patients
We investigated activation status, cytotoxic potential, and gut homing ability of the peripheral blood Natural Killer (NK) cells in Crohn disease (CD) patients. For this purpose, we compared the expression of different activating and inhibitory receptors (KIR and non-KIR) and integrins on NK cells a...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2020/6401969 |
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| author | Suzanne Samarani Patrick Sagala Prevost Jantchou Guy Grimard Christophe Faure Colette Deslandres Devendra K. Amre Ali Ahmad |
| author_facet | Suzanne Samarani Patrick Sagala Prevost Jantchou Guy Grimard Christophe Faure Colette Deslandres Devendra K. Amre Ali Ahmad |
| author_sort | Suzanne Samarani |
| collection | DOAJ |
| description | We investigated activation status, cytotoxic potential, and gut homing ability of the peripheral blood Natural Killer (NK) cells in Crohn disease (CD) patients. For this purpose, we compared the expression of different activating and inhibitory receptors (KIR and non-KIR) and integrins on NK cells as well as their recent degranulation history between the patients and age-matched healthy controls. The study was conducted using freshly obtained peripheral blood samples from the study participants. Multiple color flow cytometry was used for these determinations. Our results show that NK cells from treatment-naïve CD patients expressed higher levels of activating KIR as well as other non-KIR activating receptors vis-à-vis healthy controls. They also showed increased frequencies of the cells expressing these receptors. The expression of several KIR and non-KIR inhibitory receptors tended to decrease compared with the cells from healthy donors. NK cells from the patients also expressed increased levels of different gut-homing integrin molecules and showed a history of increased recent degranulation events both constitutively and in response to their in vitro stimulation. Furthermore, treatment of the patients tended to reverse these NK cell changes. Our results demonstrate unequivocally, for the first time, that peripheral blood NK cells in treatment-naïve CD patients are more activated and are more poised to migrate to the gut compared to their counterpart cells from healthy individuals. Moreover, they show that treatment of the patients tends to normalize their NK cells. The results suggest that NK cells are very likely to play a role in the immunopathogenesis of Crohn disease. |
| format | Article |
| id | doaj-art-38c72d5213224ea9807a2f3489f7fbc4 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-38c72d5213224ea9807a2f3489f7fbc42025-02-03T05:44:13ZengWileyMediators of Inflammation0962-93511466-18612020-01-01202010.1155/2020/64019696401969Phenotypic and Functional Changes in Peripheral Blood Natural Killer Cells in Crohn Disease PatientsSuzanne Samarani0Patrick Sagala1Prevost Jantchou2Guy Grimard3Christophe Faure4Colette Deslandres5Devendra K. Amre6Ali Ahmad7Laboratory of Innate Immunity, University of Montreal, Montreal, Quebec, CanadaLaboratory of Innate Immunity, University of Montreal, Montreal, Quebec, CanadaCHU Sainte-Justine Research Center, University of Montreal, Montreal, Quebec, CanadaCHU Sainte-Justine Research Center, University of Montreal, Montreal, Quebec, CanadaCHU Sainte-Justine Research Center, University of Montreal, Montreal, Quebec, CanadaCHU Sainte-Justine Research Center, University of Montreal, Montreal, Quebec, CanadaCHU Sainte-Justine Research Center, University of Montreal, Montreal, Quebec, CanadaLaboratory of Innate Immunity, University of Montreal, Montreal, Quebec, CanadaWe investigated activation status, cytotoxic potential, and gut homing ability of the peripheral blood Natural Killer (NK) cells in Crohn disease (CD) patients. For this purpose, we compared the expression of different activating and inhibitory receptors (KIR and non-KIR) and integrins on NK cells as well as their recent degranulation history between the patients and age-matched healthy controls. The study was conducted using freshly obtained peripheral blood samples from the study participants. Multiple color flow cytometry was used for these determinations. Our results show that NK cells from treatment-naïve CD patients expressed higher levels of activating KIR as well as other non-KIR activating receptors vis-à-vis healthy controls. They also showed increased frequencies of the cells expressing these receptors. The expression of several KIR and non-KIR inhibitory receptors tended to decrease compared with the cells from healthy donors. NK cells from the patients also expressed increased levels of different gut-homing integrin molecules and showed a history of increased recent degranulation events both constitutively and in response to their in vitro stimulation. Furthermore, treatment of the patients tended to reverse these NK cell changes. Our results demonstrate unequivocally, for the first time, that peripheral blood NK cells in treatment-naïve CD patients are more activated and are more poised to migrate to the gut compared to their counterpart cells from healthy individuals. Moreover, they show that treatment of the patients tends to normalize their NK cells. The results suggest that NK cells are very likely to play a role in the immunopathogenesis of Crohn disease.http://dx.doi.org/10.1155/2020/6401969 |
| spellingShingle | Suzanne Samarani Patrick Sagala Prevost Jantchou Guy Grimard Christophe Faure Colette Deslandres Devendra K. Amre Ali Ahmad Phenotypic and Functional Changes in Peripheral Blood Natural Killer Cells in Crohn Disease Patients Mediators of Inflammation |
| title | Phenotypic and Functional Changes in Peripheral Blood Natural Killer Cells in Crohn Disease Patients |
| title_full | Phenotypic and Functional Changes in Peripheral Blood Natural Killer Cells in Crohn Disease Patients |
| title_fullStr | Phenotypic and Functional Changes in Peripheral Blood Natural Killer Cells in Crohn Disease Patients |
| title_full_unstemmed | Phenotypic and Functional Changes in Peripheral Blood Natural Killer Cells in Crohn Disease Patients |
| title_short | Phenotypic and Functional Changes in Peripheral Blood Natural Killer Cells in Crohn Disease Patients |
| title_sort | phenotypic and functional changes in peripheral blood natural killer cells in crohn disease patients |
| url | http://dx.doi.org/10.1155/2020/6401969 |
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