Molluscan Shell Matrix Characterization by Preparative SDS-PAGE
The glycoproteinaceous constituents of molluscan shell matrices usually resist chromatographical fractionation. We describe a protocol that overcomes this difficulty and permits collection of a large amount of shell proteins for further in vitro characterization. After dissolution of the mineral pha...
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| Format: | Article |
| Language: | English |
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Wiley
2003-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/tsw.2003.30 |
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| _version_ | 1832554212760748032 |
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| author | Frederic Marin |
| author_facet | Frederic Marin |
| author_sort | Frederic Marin |
| collection | DOAJ |
| description | The glycoproteinaceous constituents of molluscan shell matrices usually resist chromatographical fractionation. We describe a protocol that overcomes this difficulty and permits collection of a large amount of shell proteins for further in vitro characterization. After dissolution of the mineral phase, the glycoproteins are fractionated �blind� on a preparative electrophoresis. They are subsequently detected with a polyclonal antibody raised against the whole matrix. |
| format | Article |
| id | doaj-art-37a541f58bf5460cb9e9a001589b8b4a |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2003-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-37a541f58bf5460cb9e9a001589b8b4a2025-02-03T05:52:04ZengWileyThe Scientific World Journal1537-744X2003-01-01334234710.1100/tsw.2003.30Molluscan Shell Matrix Characterization by Preparative SDS-PAGEFrederic Marin0IsoTis, NV., ProfBronkhorstlaan, 10, Gebouw D, 3723 MB Bilthoven, The NetherlandsThe glycoproteinaceous constituents of molluscan shell matrices usually resist chromatographical fractionation. We describe a protocol that overcomes this difficulty and permits collection of a large amount of shell proteins for further in vitro characterization. After dissolution of the mineral phase, the glycoproteins are fractionated �blind� on a preparative electrophoresis. They are subsequently detected with a polyclonal antibody raised against the whole matrix.http://dx.doi.org/10.1100/tsw.2003.30 |
| spellingShingle | Frederic Marin Molluscan Shell Matrix Characterization by Preparative SDS-PAGE The Scientific World Journal |
| title | Molluscan Shell Matrix Characterization by Preparative SDS-PAGE |
| title_full | Molluscan Shell Matrix Characterization by Preparative SDS-PAGE |
| title_fullStr | Molluscan Shell Matrix Characterization by Preparative SDS-PAGE |
| title_full_unstemmed | Molluscan Shell Matrix Characterization by Preparative SDS-PAGE |
| title_short | Molluscan Shell Matrix Characterization by Preparative SDS-PAGE |
| title_sort | molluscan shell matrix characterization by preparative sds page |
| url | http://dx.doi.org/10.1100/tsw.2003.30 |
| work_keys_str_mv | AT fredericmarin molluscanshellmatrixcharacterizationbypreparativesdspage |