Phytochemical Characterization, In Vitro Antioxidant Activity, and Quantitative Analysis by Micellar Electrokinetic Chromatography of Hawthorn (Crataegus pubescens) Fruit
Due to their antioxidant properties, polyphenolic compounds are considered beneficial for human health. In this work, we investigated the polyphenol profile and antioxidant activity of edible tejocote (Crataegus pubescens) fruit extracts by micellar electrokinetic chromatography (MEKC) and HPLC/UV....
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Food Quality |
| Online Access: | http://dx.doi.org/10.1155/2018/2154893 |
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| author | Francisco Erik González-Jiménez Juan Alfredo Salazar-Montoya Graciano Calva-Calva Emma Gloria Ramos-Ramírez |
| author_facet | Francisco Erik González-Jiménez Juan Alfredo Salazar-Montoya Graciano Calva-Calva Emma Gloria Ramos-Ramírez |
| author_sort | Francisco Erik González-Jiménez |
| collection | DOAJ |
| description | Due to their antioxidant properties, polyphenolic compounds are considered beneficial for human health. In this work, we investigated the polyphenol profile and antioxidant activity of edible tejocote (Crataegus pubescens) fruit extracts by micellar electrokinetic chromatography (MEKC) and HPLC/UV. The major phenolic compounds in the pulp extracts were (+)-catechin (9.17 ± 0.20 mg/100 mg dry fruit), (−)-epicatechin (4.32 ± 0.11 mg/100 mg dry fruit), and chlorogenic acid (5.60 ± 0.24 mg/100 mg dry fruit). The total phenolic content was 168.6 ± 0.9 mg gallic acid equivalent/g dry fruit; the total proanthocyanidin content was 84.6 ± 1.4 mg cyanidin/100 g dry fruit; and the total flavonoid content was 55.89 ± 1.43 mg quercetin/g dry fruit. Interestingly, procyanidins (dimers, trimers, and tetramers of (−)-epicatechin and (+)-catechin) were detected in the extract. This is the first study reporting the presence of polymeric polyphenols in Crataegus pubescens fruit. Accordingly, these fruits demonstrate great potential as a natural source of antioxidant phenolic compounds and could therefore be used as a nutraceutical and functional food. |
| format | Article |
| id | doaj-art-36eba7e01a9f467ab5fbd4b553c07a9d |
| institution | Kabale University |
| issn | 0146-9428 1745-4557 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Food Quality |
| spelling | doaj-art-36eba7e01a9f467ab5fbd4b553c07a9d2025-02-03T01:21:50ZengWileyJournal of Food Quality0146-94281745-45572018-01-01201810.1155/2018/21548932154893Phytochemical Characterization, In Vitro Antioxidant Activity, and Quantitative Analysis by Micellar Electrokinetic Chromatography of Hawthorn (Crataegus pubescens) FruitFrancisco Erik González-Jiménez0Juan Alfredo Salazar-Montoya1Graciano Calva-Calva2Emma Gloria Ramos-Ramírez3Department of Biotechnology and Bioengineering, Center for Research and Advanced Studies, National Polytechnic Institute, CINVESTAV-IPN, Av IPN 2508, Col. San Pedro Zacatenco, 07360 Mexico City, MexicoDepartment of Biotechnology and Bioengineering, Center for Research and Advanced Studies, National Polytechnic Institute, CINVESTAV-IPN, Av IPN 2508, Col. San Pedro Zacatenco, 07360 Mexico City, MexicoDepartment of Biotechnology and Bioengineering, Center for Research and Advanced Studies, National Polytechnic Institute, CINVESTAV-IPN, Av IPN 2508, Col. San Pedro Zacatenco, 07360 Mexico City, MexicoDepartment of Biotechnology and Bioengineering, Center for Research and Advanced Studies, National Polytechnic Institute, CINVESTAV-IPN, Av IPN 2508, Col. San Pedro Zacatenco, 07360 Mexico City, MexicoDue to their antioxidant properties, polyphenolic compounds are considered beneficial for human health. In this work, we investigated the polyphenol profile and antioxidant activity of edible tejocote (Crataegus pubescens) fruit extracts by micellar electrokinetic chromatography (MEKC) and HPLC/UV. The major phenolic compounds in the pulp extracts were (+)-catechin (9.17 ± 0.20 mg/100 mg dry fruit), (−)-epicatechin (4.32 ± 0.11 mg/100 mg dry fruit), and chlorogenic acid (5.60 ± 0.24 mg/100 mg dry fruit). The total phenolic content was 168.6 ± 0.9 mg gallic acid equivalent/g dry fruit; the total proanthocyanidin content was 84.6 ± 1.4 mg cyanidin/100 g dry fruit; and the total flavonoid content was 55.89 ± 1.43 mg quercetin/g dry fruit. Interestingly, procyanidins (dimers, trimers, and tetramers of (−)-epicatechin and (+)-catechin) were detected in the extract. This is the first study reporting the presence of polymeric polyphenols in Crataegus pubescens fruit. Accordingly, these fruits demonstrate great potential as a natural source of antioxidant phenolic compounds and could therefore be used as a nutraceutical and functional food.http://dx.doi.org/10.1155/2018/2154893 |
| spellingShingle | Francisco Erik González-Jiménez Juan Alfredo Salazar-Montoya Graciano Calva-Calva Emma Gloria Ramos-Ramírez Phytochemical Characterization, In Vitro Antioxidant Activity, and Quantitative Analysis by Micellar Electrokinetic Chromatography of Hawthorn (Crataegus pubescens) Fruit Journal of Food Quality |
| title | Phytochemical Characterization, In Vitro Antioxidant Activity, and Quantitative Analysis by Micellar Electrokinetic Chromatography of Hawthorn (Crataegus pubescens) Fruit |
| title_full | Phytochemical Characterization, In Vitro Antioxidant Activity, and Quantitative Analysis by Micellar Electrokinetic Chromatography of Hawthorn (Crataegus pubescens) Fruit |
| title_fullStr | Phytochemical Characterization, In Vitro Antioxidant Activity, and Quantitative Analysis by Micellar Electrokinetic Chromatography of Hawthorn (Crataegus pubescens) Fruit |
| title_full_unstemmed | Phytochemical Characterization, In Vitro Antioxidant Activity, and Quantitative Analysis by Micellar Electrokinetic Chromatography of Hawthorn (Crataegus pubescens) Fruit |
| title_short | Phytochemical Characterization, In Vitro Antioxidant Activity, and Quantitative Analysis by Micellar Electrokinetic Chromatography of Hawthorn (Crataegus pubescens) Fruit |
| title_sort | phytochemical characterization in vitro antioxidant activity and quantitative analysis by micellar electrokinetic chromatography of hawthorn crataegus pubescens fruit |
| url | http://dx.doi.org/10.1155/2018/2154893 |
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