Mycobacterium avium subsp. paratuberculosis in an Italian Cohort of Type 1 Diabetes Pediatric Patients
Mycobacterium avium subsp. paratuberculosis (MAP) is the etiological agent of Johne’s disease in ruminants. Recent studies have linked MAP to type 1 diabetes (T1D) in the Sardinian population. The aim of this study was to investigate the prevalence of MAP infection in a T1D cohort from continental I...
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Wiley
2012-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2012/785262 |
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| author | Maria Luisa Manca Bitti Speranza Masala Francesca Capasso Novella Rapini Simona Piccinini Federica Angelini Andrea Pierantozzi Roberta Lidano Silvia Pietrosanti Daniela Paccagnini Leonardo A. Sechi |
| author_facet | Maria Luisa Manca Bitti Speranza Masala Francesca Capasso Novella Rapini Simona Piccinini Federica Angelini Andrea Pierantozzi Roberta Lidano Silvia Pietrosanti Daniela Paccagnini Leonardo A. Sechi |
| author_sort | Maria Luisa Manca Bitti |
| collection | DOAJ |
| description | Mycobacterium avium subsp. paratuberculosis (MAP) is the etiological agent of Johne’s disease in ruminants. Recent studies have linked MAP to type 1 diabetes (T1D) in the Sardinian population. The aim of this study was to investigate the prevalence of MAP infection in a T1D cohort from continental Italy compared with healthy control subjects. 247 T1D subjects and 110 healthy controls were tested for the presence of MAP. MAP DNA was detected using IS900-specific polymerase chain reaction (PCR). The presence of antibodies towards a MAP antigen, heparin binding hemoagglutinin (HBHA), was detected by ELISA. We demonstrated a higher MAP DNA prevalence in plasma samples from T1D patients and a stronger immune response towards MAP HBHA, compared with healthy control subjects. Moreover, in the recent onset patients, we observed an association between anti-MAP antibodies and HLA DQ2 (DQA1 0201/DQB1 0202).
These findings taken together support the hypothesis of MAP as an environmental risk factor for the development of T1D in genetically predisposed subjects, probably involving a mechanism of molecular mimicry between MAP antigens and pancreatic islet β-cells. |
| format | Article |
| id | doaj-art-36e66d6b3a604f4da898fc6a39e21994 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-36e66d6b3a604f4da898fc6a39e219942025-02-03T05:44:59ZengWileyClinical and Developmental Immunology1740-25221740-25302012-01-01201210.1155/2012/785262785262Mycobacterium avium subsp. paratuberculosis in an Italian Cohort of Type 1 Diabetes Pediatric PatientsMaria Luisa Manca Bitti0Speranza Masala1Francesca Capasso2Novella Rapini3Simona Piccinini4Federica Angelini5Andrea Pierantozzi6Roberta Lidano7Silvia Pietrosanti8Daniela Paccagnini9Leonardo A. Sechi10Pediatric Diabetology Unit, Policlinico di Tor Vergata, University of Rome Tor Vergata, 00133 Rome, ItalyDipartimento di Scienze Biomediche, Università di Sassari, 07100 Sassari, ItalyDepartment of Pediatrics, University of Rome Tor Vergata, Children's Hospital Bambino Gesù, Piazza Sant’Onofrio 4, Rome, ItalyPediatric Diabetology Unit, Policlinico di Tor Vergata, University of Rome Tor Vergata, 00133 Rome, ItalyPediatric Diabetology Unit, Policlinico di Tor Vergata, University of Rome Tor Vergata, 00133 Rome, ItalyDivision of Pediatrics, Department of Public Health and Cell Biology, University of Rome Tor Vergata, 00165 Rome, ItalyDepartment of Internal Medicine, University of Rome Tor Vergata, 00133 Rome, ItalyPediatric Diabetology Unit, Policlinico di Tor Vergata, University of Rome Tor Vergata, 00133 Rome, ItalyPediatric Diabetology Unit, Policlinico di Tor Vergata, University of Rome Tor Vergata, 00133 Rome, ItalyDipartimento di Scienze Biomediche, Università di Sassari, 07100 Sassari, ItalyDipartimento di Scienze Biomediche, Università di Sassari, 07100 Sassari, ItalyMycobacterium avium subsp. paratuberculosis (MAP) is the etiological agent of Johne’s disease in ruminants. Recent studies have linked MAP to type 1 diabetes (T1D) in the Sardinian population. The aim of this study was to investigate the prevalence of MAP infection in a T1D cohort from continental Italy compared with healthy control subjects. 247 T1D subjects and 110 healthy controls were tested for the presence of MAP. MAP DNA was detected using IS900-specific polymerase chain reaction (PCR). The presence of antibodies towards a MAP antigen, heparin binding hemoagglutinin (HBHA), was detected by ELISA. We demonstrated a higher MAP DNA prevalence in plasma samples from T1D patients and a stronger immune response towards MAP HBHA, compared with healthy control subjects. Moreover, in the recent onset patients, we observed an association between anti-MAP antibodies and HLA DQ2 (DQA1 0201/DQB1 0202). These findings taken together support the hypothesis of MAP as an environmental risk factor for the development of T1D in genetically predisposed subjects, probably involving a mechanism of molecular mimicry between MAP antigens and pancreatic islet β-cells.http://dx.doi.org/10.1155/2012/785262 |
| spellingShingle | Maria Luisa Manca Bitti Speranza Masala Francesca Capasso Novella Rapini Simona Piccinini Federica Angelini Andrea Pierantozzi Roberta Lidano Silvia Pietrosanti Daniela Paccagnini Leonardo A. Sechi Mycobacterium avium subsp. paratuberculosis in an Italian Cohort of Type 1 Diabetes Pediatric Patients Clinical and Developmental Immunology |
| title | Mycobacterium avium subsp. paratuberculosis in an Italian Cohort of Type 1 Diabetes Pediatric Patients |
| title_full | Mycobacterium avium subsp. paratuberculosis in an Italian Cohort of Type 1 Diabetes Pediatric Patients |
| title_fullStr | Mycobacterium avium subsp. paratuberculosis in an Italian Cohort of Type 1 Diabetes Pediatric Patients |
| title_full_unstemmed | Mycobacterium avium subsp. paratuberculosis in an Italian Cohort of Type 1 Diabetes Pediatric Patients |
| title_short | Mycobacterium avium subsp. paratuberculosis in an Italian Cohort of Type 1 Diabetes Pediatric Patients |
| title_sort | mycobacterium avium subsp paratuberculosis in an italian cohort of type 1 diabetes pediatric patients |
| url | http://dx.doi.org/10.1155/2012/785262 |
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