The Effects of MicroRNAs on Cardiomyopathy in a Rat Model of Streptozotocin-induced Diabetes Mellitus
Objective: Diabetic cardiomyopathy (DCM) is characterized by complex pathophysiological events. miRNAs play a role in the DCM. In our study, the potential of miRNAs as a biomarker in the diagnosis and treatment of DCM was evaluated in the diabetes model induced by streptozotocin in rats. Methods: T...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Galenos Publishing House
2025-01-01
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| Series: | Bezmiâlem Science |
| Subjects: | |
| Online Access: | https://www.bezmialemscience.org/articles/the-effects-of-micrornas-on-cardiomyopathy-in-a-rat-model-of-streptozotocin-induced-diabetes-mellitus/doi/bas.galenos.2024.74429 |
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| Summary: | Objective: Diabetic cardiomyopathy (DCM) is characterized by complex pathophysiological events. miRNAs play a role in the DCM. In our study, the potential of miRNAs as a biomarker in the diagnosis and treatment of DCM was evaluated in the diabetes model induced by streptozotocin in rats.
Methods: Therefore, miRNAs obtained from rat heart tissue were analyzed by microarray, and twelve miRNAs (rno-miR-200c-3p, rno-miR-129-5p, rno-miR-150-3p, rno-miR-3584-5p, rno-miR-34c-3p, rno-miR-342-3p, rno-miR-466b-3p, rno-miR-466c-3p, rno-miR-31a-3p, rno-miR-15b-5p, rno-miR-196b-3p, and rno-miR-208a-5p) with changed expression levels were validated by real-time polymerase chain reaction analysis.
Results: As a result of the validation, it was determined that three miRNAs (rno-miR-15b-5p, rno-miR-196b-3p, and rno-miR-208a-5p) were downregulated, one miRNA (rno-miR-200c-3p) was upregulated (p<0.05). MiR-15b-5p, miR-196b-3p, miR-200c-3p, and miR-15b-5p are involved in the regulation of DCM, and the GRAP2 gene is one of the possible targets of these miRNAs.
Conclusion: miR-200c-3p has diagnostic value and may be a biomarker candidate. |
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| ISSN: | 2148-2373 |