应用非同位素aPCR-SSCP法检测P53基因突变

建立一种非同位素的不对称聚合酶链反应-单链构象多态性技术(aPCR-SSCP):通过不对称PCR(aPCR)获得单链,普通PAGE电泳分离,经银染快速准确检出突变;应用这种方法,研究了4株鼻咽癌细胞株CNE1,CNE2,HK1和SUNE1中肿瘤抑制基因P53基因突变,证实CNE1,CNE2在第8外显子,HK1在第5外显子有突变,并发现新建立的细胞株SUNE1存在第8外显子的突变。...

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Bibliographic Details
Format: Article
Language:zho
Published: Editorial Office of Journal of Sun Yat-sen University 1994-01-01
Series:Zhongshan Daxue xuebao. Yixue kexue ban
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Online Access:http://xuebaoyx.sysu.edu.cn/zh/article/43590876/
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