Green Spectrophotometric Determination of Organophosphate in Selected Fruits and Vegetables
A simple, sensitive, precise, and environmentally safe spectrophotometry method was developed and validated for the determination of organophosphate in various fruits and vegetables using a UV-Visible spectrophotometer using a magnesia mixture. The volume of reagent used for analysis and the stabili...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-01-01
|
| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2023/6691659 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832547138293202944 |
|---|---|
| author | Hemraj Sharma Hari Prasad Sapkota Kanchan Paudel Anusha Raila Sujata Kandel Pramod Chaudhary Kritika Bhattarai |
| author_facet | Hemraj Sharma Hari Prasad Sapkota Kanchan Paudel Anusha Raila Sujata Kandel Pramod Chaudhary Kritika Bhattarai |
| author_sort | Hemraj Sharma |
| collection | DOAJ |
| description | A simple, sensitive, precise, and environmentally safe spectrophotometry method was developed and validated for the determination of organophosphate in various fruits and vegetables using a UV-Visible spectrophotometer using a magnesia mixture. The volume of reagent used for analysis and the stability of the color complex were also optimized. The drug showed a stable white color complex at 420 nm. The greenness of the methods was estimated using an ecoscale (84), the Green Analytical Procedure Index, and AGREE (0.89), which were found to be excellent green method based on spectrophotometric determination. The method was validated using ICH guidelines and has acceptable values for linearity (0.5–2.5 mg/ml), accuracy (98.5–102.5%), precision, robustness, limit of detection (0.16 mg), and limit of quantification (0.486 mg). The concentration of the organophosphate in the analyzed sample was in the range of 0.003 to 2.45 mg. Altogether, the proposed green analytical method was found to be a simple, selective, sensitive, accurate, and ecofriendly method for the analysis of organophosphate in various fruits and vegetables. |
| format | Article |
| id | doaj-art-361f64ee355245b8a20e28d75759d9e3 |
| institution | Kabale University |
| issn | 1687-8779 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-361f64ee355245b8a20e28d75759d9e32025-02-03T06:45:41ZengWileyInternational Journal of Analytical Chemistry1687-87792023-01-01202310.1155/2023/6691659Green Spectrophotometric Determination of Organophosphate in Selected Fruits and VegetablesHemraj Sharma0Hari Prasad Sapkota1Kanchan Paudel2Anusha Raila3Sujata Kandel4Pramod Chaudhary5Kritika Bhattarai6Department of PharmacyDepartment of PharmacyDepartment of PharmacyDepartment of PharmacyDepartment of PharmacyDepartment of PharmacyNepal Medical College Teaching HospitalA simple, sensitive, precise, and environmentally safe spectrophotometry method was developed and validated for the determination of organophosphate in various fruits and vegetables using a UV-Visible spectrophotometer using a magnesia mixture. The volume of reagent used for analysis and the stability of the color complex were also optimized. The drug showed a stable white color complex at 420 nm. The greenness of the methods was estimated using an ecoscale (84), the Green Analytical Procedure Index, and AGREE (0.89), which were found to be excellent green method based on spectrophotometric determination. The method was validated using ICH guidelines and has acceptable values for linearity (0.5–2.5 mg/ml), accuracy (98.5–102.5%), precision, robustness, limit of detection (0.16 mg), and limit of quantification (0.486 mg). The concentration of the organophosphate in the analyzed sample was in the range of 0.003 to 2.45 mg. Altogether, the proposed green analytical method was found to be a simple, selective, sensitive, accurate, and ecofriendly method for the analysis of organophosphate in various fruits and vegetables.http://dx.doi.org/10.1155/2023/6691659 |
| spellingShingle | Hemraj Sharma Hari Prasad Sapkota Kanchan Paudel Anusha Raila Sujata Kandel Pramod Chaudhary Kritika Bhattarai Green Spectrophotometric Determination of Organophosphate in Selected Fruits and Vegetables International Journal of Analytical Chemistry |
| title | Green Spectrophotometric Determination of Organophosphate in Selected Fruits and Vegetables |
| title_full | Green Spectrophotometric Determination of Organophosphate in Selected Fruits and Vegetables |
| title_fullStr | Green Spectrophotometric Determination of Organophosphate in Selected Fruits and Vegetables |
| title_full_unstemmed | Green Spectrophotometric Determination of Organophosphate in Selected Fruits and Vegetables |
| title_short | Green Spectrophotometric Determination of Organophosphate in Selected Fruits and Vegetables |
| title_sort | green spectrophotometric determination of organophosphate in selected fruits and vegetables |
| url | http://dx.doi.org/10.1155/2023/6691659 |
| work_keys_str_mv | AT hemrajsharma greenspectrophotometricdeterminationoforganophosphateinselectedfruitsandvegetables AT hariprasadsapkota greenspectrophotometricdeterminationoforganophosphateinselectedfruitsandvegetables AT kanchanpaudel greenspectrophotometricdeterminationoforganophosphateinselectedfruitsandvegetables AT anusharaila greenspectrophotometricdeterminationoforganophosphateinselectedfruitsandvegetables AT sujatakandel greenspectrophotometricdeterminationoforganophosphateinselectedfruitsandvegetables AT pramodchaudhary greenspectrophotometricdeterminationoforganophosphateinselectedfruitsandvegetables AT kritikabhattarai greenspectrophotometricdeterminationoforganophosphateinselectedfruitsandvegetables |