Effect of a high-fat diet and iron overload on erythropoiesis in mice
Background: Insulin and iron availability stimulate and regulate erythropoiesis, respectively. The effects of hyperinsulinemia and/or iron overload on erythroid differentiation are unclear. Methodology: Male C57Bl/6J wild-type (WT) mice were fed a high-fat diet (HFD) (to produce hyperinsulinemia) or...
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Elsevier
2025-03-01
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| Series: | Biochemistry and Biophysics Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580825000068 |
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| author | Joe Varghese Jithu James Varghese Molly Jacob |
| author_facet | Joe Varghese Jithu James Varghese Molly Jacob |
| author_sort | Joe Varghese |
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| description | Background: Insulin and iron availability stimulate and regulate erythropoiesis, respectively. The effects of hyperinsulinemia and/or iron overload on erythroid differentiation are unclear. Methodology: Male C57Bl/6J wild-type (WT) mice were fed a high-fat diet (HFD) (to produce hyperinsulinemia) or a control diet (CD) for varying periods (4–24 weeks). Hepcidin knock-out (Hamp1−/−) mice (which are iron-overloaded) were fed CD or HFD for 24 weeks. Terminal erythroid differentiation (TED) in the bone marrow (BM) from these mice was analyzed by flow cytometry. Hematological parameters were estimated in peripheral blood. Results: HFD-feeding of WT mice did not significantly affect erythroid precursors in the BM or hematological parameters. However, these mice had a significantly higher reticulocyte population in the BM than those fed CD (at all time points studied). Values of hematological parameters were higher in Hamp1−/− mice than WT mice, at 24 weeks of feeding (irrespective of diet type), indicating increased erythropoiesis. Early erythroid precursors in the BM were higher in HFD-fed Hamp1−/− mice than those fed CD. Conclusions: HFD-feeding in WT mice resulted in increases in the proportion of reticulocytes in the bone marrow; maturation of the early erythroid precursors was not significantly affected. In Hamp1−/− mice, HFD-feeding increased the number of early erythroid precursors. |
| format | Article |
| id | doaj-art-34fc2ea8d3024692bd78444f970aabe6 |
| institution | Kabale University |
| issn | 2405-5808 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
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| series | Biochemistry and Biophysics Reports |
| spelling | doaj-art-34fc2ea8d3024692bd78444f970aabe62025-02-02T05:27:44ZengElsevierBiochemistry and Biophysics Reports2405-58082025-03-0141101919Effect of a high-fat diet and iron overload on erythropoiesis in miceJoe Varghese0Jithu James Varghese1Molly Jacob2Department of Biochemistry, Christian Medical College, Vellore, Tamil Nadu, India1Department of Biochemistry, Christian Medical College, Vellore, Tamil Nadu, India1Corresponding author. Department of Biochemistry, Christian Medical College, Vellore, Tamil Nadu, 632002, India.; Department of Biochemistry, Christian Medical College, Vellore, Tamil Nadu, India1Background: Insulin and iron availability stimulate and regulate erythropoiesis, respectively. The effects of hyperinsulinemia and/or iron overload on erythroid differentiation are unclear. Methodology: Male C57Bl/6J wild-type (WT) mice were fed a high-fat diet (HFD) (to produce hyperinsulinemia) or a control diet (CD) for varying periods (4–24 weeks). Hepcidin knock-out (Hamp1−/−) mice (which are iron-overloaded) were fed CD or HFD for 24 weeks. Terminal erythroid differentiation (TED) in the bone marrow (BM) from these mice was analyzed by flow cytometry. Hematological parameters were estimated in peripheral blood. Results: HFD-feeding of WT mice did not significantly affect erythroid precursors in the BM or hematological parameters. However, these mice had a significantly higher reticulocyte population in the BM than those fed CD (at all time points studied). Values of hematological parameters were higher in Hamp1−/− mice than WT mice, at 24 weeks of feeding (irrespective of diet type), indicating increased erythropoiesis. Early erythroid precursors in the BM were higher in HFD-fed Hamp1−/− mice than those fed CD. Conclusions: HFD-feeding in WT mice resulted in increases in the proportion of reticulocytes in the bone marrow; maturation of the early erythroid precursors was not significantly affected. In Hamp1−/− mice, HFD-feeding increased the number of early erythroid precursors.http://www.sciencedirect.com/science/article/pii/S2405580825000068Terminal erythroid differentiationHyperinsulinemiaHepcidinHigh-fat dietIron overload |
| spellingShingle | Joe Varghese Jithu James Varghese Molly Jacob Effect of a high-fat diet and iron overload on erythropoiesis in mice Biochemistry and Biophysics Reports Terminal erythroid differentiation Hyperinsulinemia Hepcidin High-fat diet Iron overload |
| title | Effect of a high-fat diet and iron overload on erythropoiesis in mice |
| title_full | Effect of a high-fat diet and iron overload on erythropoiesis in mice |
| title_fullStr | Effect of a high-fat diet and iron overload on erythropoiesis in mice |
| title_full_unstemmed | Effect of a high-fat diet and iron overload on erythropoiesis in mice |
| title_short | Effect of a high-fat diet and iron overload on erythropoiesis in mice |
| title_sort | effect of a high fat diet and iron overload on erythropoiesis in mice |
| topic | Terminal erythroid differentiation Hyperinsulinemia Hepcidin High-fat diet Iron overload |
| url | http://www.sciencedirect.com/science/article/pii/S2405580825000068 |
| work_keys_str_mv | AT joevarghese effectofahighfatdietandironoverloadonerythropoiesisinmice AT jithujamesvarghese effectofahighfatdietandironoverloadonerythropoiesisinmice AT mollyjacob effectofahighfatdietandironoverloadonerythropoiesisinmice |