New Spectrophotometric Determination of Tinofovir in Bulk and Pharmaceutical Dosage Form
Two new, selective and sensitive visible spectrophotometric methods (method A and B) have been developed for the estimation of tinofovir in bulk and in pharmaceutical preparations. Tinofovir was subjected to acid hydrolysis and this acid hydrolyzed drug was used for the estimation. Method A is based...
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| Format: | Article |
| Language: | English |
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Wiley
2009-01-01
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| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2009/796402 |
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| author | Manish Majumder B. Gopinath Girish Koni Sanjeev Kumar Singh |
| author_facet | Manish Majumder B. Gopinath Girish Koni Sanjeev Kumar Singh |
| author_sort | Manish Majumder |
| collection | DOAJ |
| description | Two new, selective and sensitive visible spectrophotometric methods (method A and B) have been developed for the estimation of tinofovir in bulk and in pharmaceutical preparations. Tinofovir was subjected to acid hydrolysis and this acid hydrolyzed drug was used for the estimation. Method A is based on the reaction with 3-methyl-2-benzothiazolinone hydrazone in the presence of ferric chloride, to form a colored species with a λmax at 628.5 nm. Method B is based on the reaction with Folin-ciocalteu phenol’s reagent under alkaline condition with a λmax at 768 nm. Beer’s law is obeyed in the concentration range of 5-40 µg/mL for method A and 2-30 µg/mL for method B, respectively. The methods were extended to pharmaceutical formulations and there was no interference from any common pharmaceutical excepients and diluents. The result of analysis has been validated statistically and by recovery studies. |
| format | Article |
| id | doaj-art-34e95fbd854541e296295d44c019b11a |
| institution | Kabale University |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-34e95fbd854541e296295d44c019b11a2025-02-03T01:12:26ZengWileyE-Journal of Chemistry0973-49452090-98102009-01-016253754010.1155/2009/796402New Spectrophotometric Determination of Tinofovir in Bulk and Pharmaceutical Dosage FormManish Majumder0B. Gopinath1Girish Koni2Sanjeev Kumar Singh3Department of Pharmaceutical Analysis, National College of Pharmacy, Shimoga, Karnataka, IndiaDepartment of Pharmaceutical Analysis, National College of Pharmacy, Shimoga, Karnataka, IndiaDepartment of Pharmaceutical Analysis, National College of Pharmacy, Shimoga, Karnataka, IndiaDepartment of Pharmaceutical Analysis, National College of Pharmacy, Shimoga, Karnataka, IndiaTwo new, selective and sensitive visible spectrophotometric methods (method A and B) have been developed for the estimation of tinofovir in bulk and in pharmaceutical preparations. Tinofovir was subjected to acid hydrolysis and this acid hydrolyzed drug was used for the estimation. Method A is based on the reaction with 3-methyl-2-benzothiazolinone hydrazone in the presence of ferric chloride, to form a colored species with a λmax at 628.5 nm. Method B is based on the reaction with Folin-ciocalteu phenol’s reagent under alkaline condition with a λmax at 768 nm. Beer’s law is obeyed in the concentration range of 5-40 µg/mL for method A and 2-30 µg/mL for method B, respectively. The methods were extended to pharmaceutical formulations and there was no interference from any common pharmaceutical excepients and diluents. The result of analysis has been validated statistically and by recovery studies.http://dx.doi.org/10.1155/2009/796402 |
| spellingShingle | Manish Majumder B. Gopinath Girish Koni Sanjeev Kumar Singh New Spectrophotometric Determination of Tinofovir in Bulk and Pharmaceutical Dosage Form E-Journal of Chemistry |
| title | New Spectrophotometric Determination of Tinofovir in Bulk and Pharmaceutical Dosage Form |
| title_full | New Spectrophotometric Determination of Tinofovir in Bulk and Pharmaceutical Dosage Form |
| title_fullStr | New Spectrophotometric Determination of Tinofovir in Bulk and Pharmaceutical Dosage Form |
| title_full_unstemmed | New Spectrophotometric Determination of Tinofovir in Bulk and Pharmaceutical Dosage Form |
| title_short | New Spectrophotometric Determination of Tinofovir in Bulk and Pharmaceutical Dosage Form |
| title_sort | new spectrophotometric determination of tinofovir in bulk and pharmaceutical dosage form |
| url | http://dx.doi.org/10.1155/2009/796402 |
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