The Protective Effect of Anthocyanins Extracted from Aronia Melanocarpa Berry in Renal Ischemia-Reperfusion Injury in Mice
Background. Our previous research showed the antioxidant activity of anthocyanins extracted from Aronia melanocarpa of black chokeberry in vitro. Ischemia acute kidney injury is a significant risk in developing progressive and deterioration of renal function leading to clinic chronic kidney disease....
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2021/7372893 |
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| author | Li Li Jun Li Hui Xu Fengmei Zhu Zhijun Li Hongzhi Lu Jinrong Zhang Zhengsheng Yang Yongsheng Liu |
| author_facet | Li Li Jun Li Hui Xu Fengmei Zhu Zhijun Li Hongzhi Lu Jinrong Zhang Zhengsheng Yang Yongsheng Liu |
| author_sort | Li Li |
| collection | DOAJ |
| description | Background. Our previous research showed the antioxidant activity of anthocyanins extracted from Aronia melanocarpa of black chokeberry in vitro. Ischemia acute kidney injury is a significant risk in developing progressive and deterioration of renal function leading to clinic chronic kidney disease. There were many attempts to protect the kidney against this progression of renal damage. Current study was designed to examine the effect of pretreatment with three anthocyanins named cyanidin-3-arabinoside, cyanidin-3-glucodise, and cyaniding-3-galactoside against acute ischemia-reperfusion injury in mouse kidney. Methods. Acute renal injury model was initiated by 30 min clamping bilateral renal pedicle and followed by 24-hour reperfusion in C57Bl/6J mice. Four groups of mice were orally pretreated in 50 mg/g/12 h for two weeks with cyanidin-3-arabinoside, cyanidin-3-glucodise, and cyaniding-3-galactoside and anthocyanins (three-cyanidin mixture), respectively, sham-control group and the renal injury-untreated groups only with saline. Results. The model resulted in renal dysfunction with high serum creatinine, blood urea nitrogen, and changes in proinflammatory cytokines (TNF-ɑ, IL-1β, IL-6, and MCP-1), renal oxidative stress (SOD, GSH, and CAT), lipid peroxidation (TBARS and MDA), and apoptosis (caspase-9). Pretreatment of two weeks resulted in different extent amelioration of renal dysfunction and tubular damage and suppression of proinflammatory cytokines, oxidative stress, lipid peroxidation, and apoptosis, thus suggesting that cyanidins are potentially effective in acute renal ischemia by the decrease of inflammation, oxidative stress, and lipid peroxidation, as well as apoptosis. Conclusion. the current study provided the first attempt to investigate the role of anthocyanins purified from Aronia melanocarpa berry in amelioration of acute renal failure via antioxidant and cytoprotective effects. |
| format | Article |
| id | doaj-art-34a23b6bfbda4f52be440dc6410a4b2d |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
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| series | Mediators of Inflammation |
| spelling | doaj-art-34a23b6bfbda4f52be440dc6410a4b2d2025-02-03T01:00:41ZengWileyMediators of Inflammation0962-93511466-18612021-01-01202110.1155/2021/73728937372893The Protective Effect of Anthocyanins Extracted from Aronia Melanocarpa Berry in Renal Ischemia-Reperfusion Injury in MiceLi Li0Jun Li1Hui Xu2Fengmei Zhu3Zhijun Li4Hongzhi Lu5Jinrong Zhang6Zhengsheng Yang7Yongsheng Liu8Department of Internal Medicine, The First Hospital of Qinhuangdao, No. 258 Wenhua Road, Haigang, Qinhuangdao, Hebei, 066000, ChinaHebei Normal University of Science and Technology, No. 360, Hebeidajie, Haigang, Qinhuangdao, Hebei, 066000, ChinaDepartment of Internal Medicine, The First Hospital of Qinhuangdao, No. 258 Wenhua Road, Haigang, Qinhuangdao, Hebei, 066000, ChinaHebei Normal University of Science and Technology, No. 360, Hebeidajie, Haigang, Qinhuangdao, Hebei, 066000, ChinaThe People Hospital of Shanhaiguan, No. 5, Guancheng Road, Shanhaiguan, Qinhuangdao, Hebei, 066200, ChinaDepartment of Internal Medicine, The First Hospital of Qinhuangdao, No. 258 Wenhua Road, Haigang, Qinhuangdao, Hebei, 066000, ChinaDepartment of Internal Medicine, The First Hospital of Qinhuangdao, No. 258 Wenhua Road, Haigang, Qinhuangdao, Hebei, 066000, ChinaDepartment of Internal Medicine, The First Hospital of Qinhuangdao, No. 258 Wenhua Road, Haigang, Qinhuangdao, Hebei, 066000, ChinaDepartment of Internal Medicine, The First Hospital of Qinhuangdao, No. 258 Wenhua Road, Haigang, Qinhuangdao, Hebei, 066000, ChinaBackground. Our previous research showed the antioxidant activity of anthocyanins extracted from Aronia melanocarpa of black chokeberry in vitro. Ischemia acute kidney injury is a significant risk in developing progressive and deterioration of renal function leading to clinic chronic kidney disease. There were many attempts to protect the kidney against this progression of renal damage. Current study was designed to examine the effect of pretreatment with three anthocyanins named cyanidin-3-arabinoside, cyanidin-3-glucodise, and cyaniding-3-galactoside against acute ischemia-reperfusion injury in mouse kidney. Methods. Acute renal injury model was initiated by 30 min clamping bilateral renal pedicle and followed by 24-hour reperfusion in C57Bl/6J mice. Four groups of mice were orally pretreated in 50 mg/g/12 h for two weeks with cyanidin-3-arabinoside, cyanidin-3-glucodise, and cyaniding-3-galactoside and anthocyanins (three-cyanidin mixture), respectively, sham-control group and the renal injury-untreated groups only with saline. Results. The model resulted in renal dysfunction with high serum creatinine, blood urea nitrogen, and changes in proinflammatory cytokines (TNF-ɑ, IL-1β, IL-6, and MCP-1), renal oxidative stress (SOD, GSH, and CAT), lipid peroxidation (TBARS and MDA), and apoptosis (caspase-9). Pretreatment of two weeks resulted in different extent amelioration of renal dysfunction and tubular damage and suppression of proinflammatory cytokines, oxidative stress, lipid peroxidation, and apoptosis, thus suggesting that cyanidins are potentially effective in acute renal ischemia by the decrease of inflammation, oxidative stress, and lipid peroxidation, as well as apoptosis. Conclusion. the current study provided the first attempt to investigate the role of anthocyanins purified from Aronia melanocarpa berry in amelioration of acute renal failure via antioxidant and cytoprotective effects.http://dx.doi.org/10.1155/2021/7372893 |
| spellingShingle | Li Li Jun Li Hui Xu Fengmei Zhu Zhijun Li Hongzhi Lu Jinrong Zhang Zhengsheng Yang Yongsheng Liu The Protective Effect of Anthocyanins Extracted from Aronia Melanocarpa Berry in Renal Ischemia-Reperfusion Injury in Mice Mediators of Inflammation |
| title | The Protective Effect of Anthocyanins Extracted from Aronia Melanocarpa Berry in Renal Ischemia-Reperfusion Injury in Mice |
| title_full | The Protective Effect of Anthocyanins Extracted from Aronia Melanocarpa Berry in Renal Ischemia-Reperfusion Injury in Mice |
| title_fullStr | The Protective Effect of Anthocyanins Extracted from Aronia Melanocarpa Berry in Renal Ischemia-Reperfusion Injury in Mice |
| title_full_unstemmed | The Protective Effect of Anthocyanins Extracted from Aronia Melanocarpa Berry in Renal Ischemia-Reperfusion Injury in Mice |
| title_short | The Protective Effect of Anthocyanins Extracted from Aronia Melanocarpa Berry in Renal Ischemia-Reperfusion Injury in Mice |
| title_sort | protective effect of anthocyanins extracted from aronia melanocarpa berry in renal ischemia reperfusion injury in mice |
| url | http://dx.doi.org/10.1155/2021/7372893 |
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