Billion‐Scale Expansion of Functional hiPSC‐Derived Cardiomyocytes in Bioreactors Through Oxygen Control and Continuous Wnt Activation

Billion‐Scale Expansion of Functional hiPSC‐Derived Cardiomyocytes in Bioreactors Through Oxygen Control and Continuous Wnt Activation

Abstract Generation of upscaled quantities of human‐induced pluripotent stem cell‐derived cardiomyocytes (hiPSC‐CM), for therapeutic or testing applications, is both expensive and time‐consuming. Herein, a scalable bioprocess for hiPSC‐CM expansion in stirred‐tank bioreactors (STB) is developed. By...

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Main Authors: Pedro Vicente, Lara R. Inocêncio, Asier Ullate‐Agote, Ana F. Louro, João Jacinto, Beatriz Gamelas, Olalla Iglesias‐García, Patxi San Martin‐Uriz, Paula Aguirre‐Ruiz, Gonzalo R. Ríos‐Muñoz, María Eugenia Fernández‐Santos, Alain vanMil, Joost P. G. Sluijter, Felipe Prósper, Manuel M Mazo Vega, Paula M. Alves, Margarida Serra
Format: Article
Language:English
Published: Wiley 2025-03-01
Series:Advanced Science
Subjects:
3D cell culture
bioprocess scale‐up
hiPSC‐CM expansion
mild hypoxia
stirred‐tank bioreactor
suspension culture
Online Access:https://doi.org/10.1002/advs.202410510
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https://doi.org/10.1002/advs.202410510

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