Oligonucleotide and Parylene Surface Coating of Polystyrene and ePTFE for Improved Endothelial Cell Attachment and Hemocompatibility
In vivo self-endothelialization by endothelial cell adhesion on cardiovascular implants is highly desirable. DNA-oligonucleotides are an intriguing coating material with nonimmunogenic characteristics and the feasibility of easy and rapid chemical fabrication. The objective of this study was the cre...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | International Journal of Biomaterials |
| Online Access: | http://dx.doi.org/10.1155/2012/397813 |
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| author | Martina Schleicher Jan Hansmann Bentsian Elkin Petra J. Kluger Simone Liebscher Agnes J. T. Huber Olaf Fritze Christine Schille Michaela Müller Katja Schenke-Layland Martina Seifert Heike Walles Hans-Peter Wendel Ulrich A. Stock |
| author_facet | Martina Schleicher Jan Hansmann Bentsian Elkin Petra J. Kluger Simone Liebscher Agnes J. T. Huber Olaf Fritze Christine Schille Michaela Müller Katja Schenke-Layland Martina Seifert Heike Walles Hans-Peter Wendel Ulrich A. Stock |
| author_sort | Martina Schleicher |
| collection | DOAJ |
| description | In vivo self-endothelialization by endothelial cell adhesion on cardiovascular implants is highly desirable. DNA-oligonucleotides are an intriguing coating material with nonimmunogenic characteristics and the feasibility of easy and rapid chemical fabrication. The objective of this study was the creation of cell adhesive DNA-oligonucleotide coatings on vascular implant surfaces. DNA-oligonucleotides immobilized by adsorption on parylene (poly(monoaminomethyl-para-xylene)) coated polystyrene and ePTFE were resistant to high shear stress (9.5 N/m2) and human blood serum for up to 96 h. Adhesion of murine endothelial progenitor cells, HUVECs and endothelial cells from human adult saphenous veins as well as viability over a period of 14 days of HUVECs on oligonucleotide coated samples under dynamic culture conditions was significantly enhanced (P<0.05). Oligonucleotide-coated surfaces revealed low thrombogenicity and excellent hemocompatibility after incubation with human blood. These properties suggest the suitability of immobilization of DNA-oligonucleotides for biofunctionalization of blood vessel substitutes for improved in vivo endothelialization. |
| format | Article |
| id | doaj-art-33c113f44ec1473e89c85e9c9ce3d878 |
| institution | Kabale University |
| issn | 1687-8787 1687-8795 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Biomaterials |
| spelling | doaj-art-33c113f44ec1473e89c85e9c9ce3d8782025-02-03T06:01:21ZengWileyInternational Journal of Biomaterials1687-87871687-87952012-01-01201210.1155/2012/397813397813Oligonucleotide and Parylene Surface Coating of Polystyrene and ePTFE for Improved Endothelial Cell Attachment and HemocompatibilityMartina Schleicher0Jan Hansmann1Bentsian Elkin2Petra J. Kluger3Simone Liebscher4Agnes J. T. Huber5Olaf Fritze6Christine Schille7Michaela Müller8Katja Schenke-Layland9Martina Seifert10Heike Walles11Hans-Peter Wendel12Ulrich A. Stock13Department of Thoracic, Cardiac and Vascular Surgery, University Hospital, Hoppe-Seyler-Strasse 3, 72076 Tuebingen, GermanyFraunhofer Institute for Interfacial Engineering and Biotechnology, Nobelstrasse 12, 70569 Stuttgart, GermanyFraunhofer Institute for Interfacial Engineering and Biotechnology, Nobelstrasse 12, 70569 Stuttgart, GermanyFraunhofer Institute for Interfacial Engineering and Biotechnology, Nobelstrasse 12, 70569 Stuttgart, GermanyDepartment of Thoracic, Cardiac and Vascular Surgery, University Hospital, Hoppe-Seyler-Strasse 3, 72076 Tuebingen, GermanyDepartment of Thoracic, Cardiac and Vascular Surgery, University Hospital, Hoppe-Seyler-Strasse 3, 72076 Tuebingen, GermanyDepartment of Thoracic, Cardiac and Vascular Surgery, University Hospital, Hoppe-Seyler-Strasse 3, 72076 Tuebingen, GermanyDepartment of Prosthodontics, Section of Medical Materials and Technology, University Hospital, Osianderstrasse 2-8, 72076 Tübingen, GermanyFraunhofer Institute for Interfacial Engineering and Biotechnology, Nobelstrasse 12, 70569 Stuttgart, GermanyFraunhofer Institute for Interfacial Engineering and Biotechnology, Nobelstrasse 12, 70569 Stuttgart, GermanyInstitute of Medical Immunology, Charité Universitätsmedizin Berlin, Föhrer Straße 15, 13353 Berlin, GermanyFraunhofer Institute for Interfacial Engineering and Biotechnology, Nobelstrasse 12, 70569 Stuttgart, GermanyDepartment of Thoracic, Cardiac and Vascular Surgery, University Hospital, Hoppe-Seyler-Strasse 3, 72076 Tuebingen, GermanyDepartment of Thoracic, Cardiac and Vascular Surgery, University Hospital, Hoppe-Seyler-Strasse 3, 72076 Tuebingen, GermanyIn vivo self-endothelialization by endothelial cell adhesion on cardiovascular implants is highly desirable. DNA-oligonucleotides are an intriguing coating material with nonimmunogenic characteristics and the feasibility of easy and rapid chemical fabrication. The objective of this study was the creation of cell adhesive DNA-oligonucleotide coatings on vascular implant surfaces. DNA-oligonucleotides immobilized by adsorption on parylene (poly(monoaminomethyl-para-xylene)) coated polystyrene and ePTFE were resistant to high shear stress (9.5 N/m2) and human blood serum for up to 96 h. Adhesion of murine endothelial progenitor cells, HUVECs and endothelial cells from human adult saphenous veins as well as viability over a period of 14 days of HUVECs on oligonucleotide coated samples under dynamic culture conditions was significantly enhanced (P<0.05). Oligonucleotide-coated surfaces revealed low thrombogenicity and excellent hemocompatibility after incubation with human blood. These properties suggest the suitability of immobilization of DNA-oligonucleotides for biofunctionalization of blood vessel substitutes for improved in vivo endothelialization.http://dx.doi.org/10.1155/2012/397813 |
| spellingShingle | Martina Schleicher Jan Hansmann Bentsian Elkin Petra J. Kluger Simone Liebscher Agnes J. T. Huber Olaf Fritze Christine Schille Michaela Müller Katja Schenke-Layland Martina Seifert Heike Walles Hans-Peter Wendel Ulrich A. Stock Oligonucleotide and Parylene Surface Coating of Polystyrene and ePTFE for Improved Endothelial Cell Attachment and Hemocompatibility International Journal of Biomaterials |
| title | Oligonucleotide and Parylene Surface Coating of Polystyrene and ePTFE for Improved Endothelial Cell Attachment and Hemocompatibility |
| title_full | Oligonucleotide and Parylene Surface Coating of Polystyrene and ePTFE for Improved Endothelial Cell Attachment and Hemocompatibility |
| title_fullStr | Oligonucleotide and Parylene Surface Coating of Polystyrene and ePTFE for Improved Endothelial Cell Attachment and Hemocompatibility |
| title_full_unstemmed | Oligonucleotide and Parylene Surface Coating of Polystyrene and ePTFE for Improved Endothelial Cell Attachment and Hemocompatibility |
| title_short | Oligonucleotide and Parylene Surface Coating of Polystyrene and ePTFE for Improved Endothelial Cell Attachment and Hemocompatibility |
| title_sort | oligonucleotide and parylene surface coating of polystyrene and eptfe for improved endothelial cell attachment and hemocompatibility |
| url | http://dx.doi.org/10.1155/2012/397813 |
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