Expression of recombinant bovine prethrombin-2 in E. coli and optimized conditions for its production in liquid culture
Vector with gene of recombinant bovine prethrombin-2 (pThr-2) was successfully cloned into E. coli BL21 (DE3) cells. After the recombinant bacterium cells were induced by IPTG and further culture, pThr-2 inclusion bodies were obtained. The fermentation conditions for the pThr-2 production were optim...
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2005-01-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2005.01.0032 |
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| author | CHEN Jian-liang GUAN Yi-xin CUI Zhi-fang YAO Shan-jing |
| author_facet | CHEN Jian-liang GUAN Yi-xin CUI Zhi-fang YAO Shan-jing |
| author_sort | CHEN Jian-liang |
| collection | DOAJ |
| description | Vector with gene of recombinant bovine prethrombin-2 (pThr-2) was successfully cloned into E. coli BL21 (DE3) cells. After the recombinant bacterium cells were induced by IPTG and further culture, pThr-2 inclusion bodies were obtained. The fermentation conditions for the pThr-2 production were optimized. The inclusion bodies of the pThr-2 produced in the E. coli culture amounted to 110 mg/L, accounting for 21.7% of the total cell proteins. |
| format | Article |
| id | doaj-art-32f88f963ee54500a5ca0e198860e045 |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2005-01-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-32f88f963ee54500a5ca0e198860e0452025-08-20T03:34:18ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552005-01-0131323610.3785/1008-9209.2005.01.003210089209Expression of recombinant bovine prethrombin-2 in E. coli and optimized conditions for its production in liquid cultureCHEN Jian-liangGUAN Yi-xinCUI Zhi-fangYAO Shan-jingVector with gene of recombinant bovine prethrombin-2 (pThr-2) was successfully cloned into E. coli BL21 (DE3) cells. After the recombinant bacterium cells were induced by IPTG and further culture, pThr-2 inclusion bodies were obtained. The fermentation conditions for the pThr-2 production were optimized. The inclusion bodies of the pThr-2 produced in the E. coli culture amounted to 110 mg/L, accounting for 21.7% of the total cell proteins.https://www.academax.com/doi/10.3785/1008-9209.2005.01.0032recombinant bovine prethrombin-2<italic>E. coli</italic>fermentationoptimizationinclusion body |
| spellingShingle | CHEN Jian-liang GUAN Yi-xin CUI Zhi-fang YAO Shan-jing Expression of recombinant bovine prethrombin-2 in E. coli and optimized conditions for its production in liquid culture 浙江大学学报. 农业与生命科学版 recombinant bovine prethrombin-2 <italic>E. coli</italic> fermentation optimization inclusion body |
| title | Expression of recombinant bovine prethrombin-2 in E. coli and optimized conditions for its production in liquid culture |
| title_full | Expression of recombinant bovine prethrombin-2 in E. coli and optimized conditions for its production in liquid culture |
| title_fullStr | Expression of recombinant bovine prethrombin-2 in E. coli and optimized conditions for its production in liquid culture |
| title_full_unstemmed | Expression of recombinant bovine prethrombin-2 in E. coli and optimized conditions for its production in liquid culture |
| title_short | Expression of recombinant bovine prethrombin-2 in E. coli and optimized conditions for its production in liquid culture |
| title_sort | expression of recombinant bovine prethrombin 2 in e coli and optimized conditions for its production in liquid culture |
| topic | recombinant bovine prethrombin-2 <italic>E. coli</italic> fermentation optimization inclusion body |
| url | https://www.academax.com/doi/10.3785/1008-9209.2005.01.0032 |
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