Expression of recombinant bovine prethrombin-2 in E. coli and optimized conditions for its production in liquid culture

Vector with gene of recombinant bovine prethrombin-2 (pThr-2) was successfully cloned into E. coli BL21 (DE3) cells. After the recombinant bacterium cells were induced by IPTG and further culture, pThr-2 inclusion bodies were obtained. The fermentation conditions for the pThr-2 production were optim...

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Bibliographic Details
Main Authors: CHEN Jian-liang, GUAN Yi-xin, CUI Zhi-fang, YAO Shan-jing
Format: Article
Language:English
Published: Zhejiang University Press 2005-01-01
Series:浙江大学学报. 农业与生命科学版
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Online Access:https://www.academax.com/doi/10.3785/1008-9209.2005.01.0032
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