Molecular cloning of EZH2 oncogene in chronic myeloid leukemia and therapeutic prospects
Chronic myeloid leukemia (CML) is a myeloproliferative neoplasia, caused in 95 % of the cases by a cytogenetic abnormality characterized by the reciprocal translocation between chromosomes 9 and 22 t(9; 22) (q34; q11), resulting in Philadelphia chromosome (Ph).Considering that CML cure is only pos...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Centro Universitário São Camilo
2015-07-01
|
| Series: | O Mundo da Saúde |
| Subjects: | |
| Online Access: | https://revistamundodasaude.emnuvens.com.br/mundodasaude/article/view/314 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832577657985826816 |
|---|---|
| author | Juliana Costa Gaspar Lucas Santos de Santana Camila Menezes Freire de Souza Marcos Montani Caseiro Rosane Rezende de Souza Giuliani Cleide Barbieri de Souza |
| author_facet | Juliana Costa Gaspar Lucas Santos de Santana Camila Menezes Freire de Souza Marcos Montani Caseiro Rosane Rezende de Souza Giuliani Cleide Barbieri de Souza |
| author_sort | Juliana Costa Gaspar |
| collection | DOAJ |
| description | Chronic myeloid leukemia (CML) is a myeloproliferative neoplasia, caused in 95 % of the cases by a cytogenetic abnormality
characterized by the reciprocal translocation between chromosomes 9 and 22 t(9; 22) (q34; q11), resulting in Philadelphia
chromosome (Ph).Considering that CML cure is only possible with a successful bone marrow transplantation and that there
are resistance cases to the tyrosine kinase inhibitor Imatinib Mesylate, prescribed in first line drug treatment; it is important
to know in detail the genes and proteins that are possibly altered in CML, favoring effective therapeutic strategies, optimized
diagnosis and minimal residual disease detection. Among the new therapeutic approaches to CML is gene therapy, which,
depending on the target gene, can be efficient to other neoplasms treatment. In this context, the aims of this work were:
to proceed the molecular cloning of a DNA fragment from EZH2 gene, potential target for gene therapy, that promotes
epigenetic alteration in CML and in many neoplasms; as well as to contribute to studies related to interspecies gene transfer
that have high contribution to health. For this reason, the EZH2 gene was isolated from peripheral blood genomic DNA
from CML patients and cloned in prokaryotic systems. Therefore, in this study, we proceed, defined and proved the interspe-
cies gene transfer procedures and discussed the future of biotechnology in researches and treatments, especially in CML. |
| format | Article |
| id | doaj-art-32a10eafe3414a0fbf7821d4fd41c29e |
| institution | Kabale University |
| issn | 0104-7809 1980-3990 |
| language | English |
| publishDate | 2015-07-01 |
| publisher | Centro Universitário São Camilo |
| record_format | Article |
| series | O Mundo da Saúde |
| spelling | doaj-art-32a10eafe3414a0fbf7821d4fd41c29e2025-01-30T19:43:45ZengCentro Universitário São CamiloO Mundo da Saúde0104-78091980-39902015-07-01393307315258Molecular cloning of EZH2 oncogene in chronic myeloid leukemia and therapeutic prospectsJuliana Costa Gaspar0Lucas Santos de Santana1Camila Menezes Freire de Souza2Marcos Montani Caseiro3Rosane Rezende de Souza Giuliani4Cleide Barbieri de Souza5Centro Universitário Lusíada (UNILUS) – Laboratório de Biologia Molecular – UNILUS, Campus II – Santos, SP – Brasil.Unidade de Endocrinologia Genética – Faculdade de Medicina da Universidade de São Paulo (USP) – São Paulo – SP – Brasil. Instituto Butantan – São Paulo, SP – Brasil Laboratório de Biologia Molecular – UNILUS, Campus II – Santos, SP – Brasil.Hospital Guilherme Álvaro – Santos, SP – Brasil. Laboratório de Biologia Molecular – UNILUS, Campus II – Santos, SP – Brasil.Chronic myeloid leukemia (CML) is a myeloproliferative neoplasia, caused in 95 % of the cases by a cytogenetic abnormality characterized by the reciprocal translocation between chromosomes 9 and 22 t(9; 22) (q34; q11), resulting in Philadelphia chromosome (Ph).Considering that CML cure is only possible with a successful bone marrow transplantation and that there are resistance cases to the tyrosine kinase inhibitor Imatinib Mesylate, prescribed in first line drug treatment; it is important to know in detail the genes and proteins that are possibly altered in CML, favoring effective therapeutic strategies, optimized diagnosis and minimal residual disease detection. Among the new therapeutic approaches to CML is gene therapy, which, depending on the target gene, can be efficient to other neoplasms treatment. In this context, the aims of this work were: to proceed the molecular cloning of a DNA fragment from EZH2 gene, potential target for gene therapy, that promotes epigenetic alteration in CML and in many neoplasms; as well as to contribute to studies related to interspecies gene transfer that have high contribution to health. For this reason, the EZH2 gene was isolated from peripheral blood genomic DNA from CML patients and cloned in prokaryotic systems. Therefore, in this study, we proceed, defined and proved the interspe- cies gene transfer procedures and discussed the future of biotechnology in researches and treatments, especially in CML.https://revistamundodasaude.emnuvens.com.br/mundodasaude/article/view/314biotechnology. chronic myelogenous leukemia. molecular cloning. gene therapy. |
| spellingShingle | Juliana Costa Gaspar Lucas Santos de Santana Camila Menezes Freire de Souza Marcos Montani Caseiro Rosane Rezende de Souza Giuliani Cleide Barbieri de Souza Molecular cloning of EZH2 oncogene in chronic myeloid leukemia and therapeutic prospects O Mundo da Saúde biotechnology. chronic myelogenous leukemia. molecular cloning. gene therapy. |
| title | Molecular cloning of EZH2 oncogene in chronic myeloid leukemia and therapeutic prospects |
| title_full | Molecular cloning of EZH2 oncogene in chronic myeloid leukemia and therapeutic prospects |
| title_fullStr | Molecular cloning of EZH2 oncogene in chronic myeloid leukemia and therapeutic prospects |
| title_full_unstemmed | Molecular cloning of EZH2 oncogene in chronic myeloid leukemia and therapeutic prospects |
| title_short | Molecular cloning of EZH2 oncogene in chronic myeloid leukemia and therapeutic prospects |
| title_sort | molecular cloning of ezh2 oncogene in chronic myeloid leukemia and therapeutic prospects |
| topic | biotechnology. chronic myelogenous leukemia. molecular cloning. gene therapy. |
| url | https://revistamundodasaude.emnuvens.com.br/mundodasaude/article/view/314 |
| work_keys_str_mv | AT julianacostagaspar molecularcloningofezh2oncogeneinchronicmyeloidleukemiaandtherapeuticprospects AT lucassantosdesantana molecularcloningofezh2oncogeneinchronicmyeloidleukemiaandtherapeuticprospects AT camilamenezesfreiredesouza molecularcloningofezh2oncogeneinchronicmyeloidleukemiaandtherapeuticprospects AT marcosmontanicaseiro molecularcloningofezh2oncogeneinchronicmyeloidleukemiaandtherapeuticprospects AT rosanerezendedesouzagiuliani molecularcloningofezh2oncogeneinchronicmyeloidleukemiaandtherapeuticprospects AT cleidebarbieridesouza molecularcloningofezh2oncogeneinchronicmyeloidleukemiaandtherapeuticprospects |