Challenges in observing transcription–translation for bottom-up synthetic biology
Synthetic biology aims to create a viable synthetic cell. However, to achieve this goal, it is essential first to gain a profound understanding of the cellular systems used to build that cell, how to reconstitute those systems in the compartments, and how to track their function. Transcription and t...
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Cambridge University Press
2025-01-01
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| Series: | QRB Discovery |
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| Online Access: | https://www.cambridge.org/core/product/identifier/S2633289224000279/type/journal_article |
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| author | Vadim G. Bogatyr Gijs J. L. Wuite |
| author_facet | Vadim G. Bogatyr Gijs J. L. Wuite |
| author_sort | Vadim G. Bogatyr |
| collection | DOAJ |
| description | Synthetic biology aims to create a viable synthetic cell. However, to achieve this goal, it is essential first to gain a profound understanding of the cellular systems used to build that cell, how to reconstitute those systems in the compartments, and how to track their function. Transcription and translation are two vital cellular systems responsible for the production of RNA and, consequently, proteins, without which the cell would not be able to maintain itself or fulfill its functions. This review discusses in detail how the Protein synthesis Using Recombinant Element (PURE) system and cell lysate are used to reconstitute transcription–translation in vitro. Furthermore, it examines how these systems can be encapsulated in GUVs using the existing methods. It also assesses approaches available to image transcription and translation with a diverse arsenal of fluorescence microscopy techniques and a broad collection of probes developed in recent decades. Finally, it highlights solutions for the challenge ahead, namely the decoupling of the two systems in PURE, and discusses the prospects of synthetic biology in the modern world. |
| format | Article |
| id | doaj-art-32367031bcb74e9aa64b95d0c2473d37 |
| institution | Kabale University |
| issn | 2633-2892 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Cambridge University Press |
| record_format | Article |
| series | QRB Discovery |
| spelling | doaj-art-32367031bcb74e9aa64b95d0c2473d372025-01-28T08:01:13ZengCambridge University PressQRB Discovery2633-28922025-01-01610.1017/qrd.2024.27Challenges in observing transcription–translation for bottom-up synthetic biologyVadim G. Bogatyr0https://orcid.org/0000-0002-2829-3197Gijs J. L. Wuite1https://orcid.org/0000-0002-5706-043XDepartment of Physics and Astronomy and LaserLab, Faculty of Science, Vrije Universiteit, Amsterdam, NetherlandsDepartment of Physics and Astronomy and LaserLab, Faculty of Science, Vrije Universiteit, Amsterdam, NetherlandsSynthetic biology aims to create a viable synthetic cell. However, to achieve this goal, it is essential first to gain a profound understanding of the cellular systems used to build that cell, how to reconstitute those systems in the compartments, and how to track their function. Transcription and translation are two vital cellular systems responsible for the production of RNA and, consequently, proteins, without which the cell would not be able to maintain itself or fulfill its functions. This review discusses in detail how the Protein synthesis Using Recombinant Element (PURE) system and cell lysate are used to reconstitute transcription–translation in vitro. Furthermore, it examines how these systems can be encapsulated in GUVs using the existing methods. It also assesses approaches available to image transcription and translation with a diverse arsenal of fluorescence microscopy techniques and a broad collection of probes developed in recent decades. Finally, it highlights solutions for the challenge ahead, namely the decoupling of the two systems in PURE, and discusses the prospects of synthetic biology in the modern world.https://www.cambridge.org/core/product/identifier/S2633289224000279/type/journal_articleDNAtranscritpionfluorescenceRNAprotein–nucleic acid interactionstranslationsynthetic biology |
| spellingShingle | Vadim G. Bogatyr Gijs J. L. Wuite Challenges in observing transcription–translation for bottom-up synthetic biology QRB Discovery DNA transcritpion fluorescence RNA protein–nucleic acid interactions translation synthetic biology |
| title | Challenges in observing transcription–translation for bottom-up synthetic biology |
| title_full | Challenges in observing transcription–translation for bottom-up synthetic biology |
| title_fullStr | Challenges in observing transcription–translation for bottom-up synthetic biology |
| title_full_unstemmed | Challenges in observing transcription–translation for bottom-up synthetic biology |
| title_short | Challenges in observing transcription–translation for bottom-up synthetic biology |
| title_sort | challenges in observing transcription translation for bottom up synthetic biology |
| topic | DNA transcritpion fluorescence RNA protein–nucleic acid interactions translation synthetic biology |
| url | https://www.cambridge.org/core/product/identifier/S2633289224000279/type/journal_article |
| work_keys_str_mv | AT vadimgbogatyr challengesinobservingtranscriptiontranslationforbottomupsyntheticbiology AT gijsjlwuite challengesinobservingtranscriptiontranslationforbottomupsyntheticbiology |